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Pig Spermatozoa Defect in Acrosome Formation Caused Poor Motion Parameters and Fertilization Failure through Artificial Insemination and In vitro Fertilization.

Lee WY, Lee R, Kim HC, Lee KH, Cui XS, Kim NH, Kim SH, Lee IJ, Uhm SJ, Yoon MJ, Song H - Asian-australas. J. Anim. Sci. (2014)

Bottom Line: Parameters for the selection of semen mainly include total sperm motility, concentration, and morphology.The present study was designed to compare the motion characteristics, fertilization ability using in vitro fertilization (IVF), and acrosome formation of the semen from boars having low (boar number 2012) and normal (boar number 2004 and 2023) breeding performances.In conclusion, the present study suggests that the acrosome staining and detailed motion characteristics such as progressiveness, VCL, and VSL should be included in determining semen quality together with primary parameters for successful AI and high breeding performance in the swine industry.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science, College of Agriculture, Chungbuk National University, Choung-ju 361-763, Korea .

ABSTRACT
The selection of morphologically normal spermatozoa is critical to obtain high breeding performances in boar breeding farms and artificial insemination (AI) centers. Parameters for the selection of semen mainly include total sperm motility, concentration, and morphology. However, these primary parameters are often not reliable for discriminating between normal and abnormal, non-fertilizable spermatozoa. The present study was designed to compare the motion characteristics, fertilization ability using in vitro fertilization (IVF), and acrosome formation of the semen from boars having low (boar number 2012) and normal (boar number 2004 and 2023) breeding performances. The ultimate goal was to identify additional simple and easy criteria for the selection of normal sperm. There was no significant difference between boar 2004 and boar 2023 sperm total motility in computer assisted sperm analysis. However, boar number 2012 semen presented a significantly reduced population of rapid moving spermatozoa and an increased population of slow moving spermatozoa compared to boar numbers 2004 and 2023. Analysis of detailed motion characteristics revealed that sperm from boar number 2012 had significantly reduced motility in progressiveness, average path velocity, straight-line velocity (VSL), curvilinear velocity (VCL), straightness, and linearity. The assessment of the fertilizing ability by IVF also showed that sperm from boar number 2012 showed a fertility rate of 3.4%, whereas sperm from boar number 2023 had a fertility rate of 75.45%. Interestingly, most of the sperm nuclei were found on the peripheral area of the oocytes, suggesting that the sperm from boar number 2012 lacked penetration ability into the oocyte zonapellucida. The acrosome formation analysis using Pisum sativum agglutinin staining demonstrated that the sperm from boar number 2012 had a defect in acrosome formation. Consequently, primary parameters for selecting semen before AI such as motility are not sufficient to select normal and fertilizable spermatozoa. In conclusion, the present study suggests that the acrosome staining and detailed motion characteristics such as progressiveness, VCL, and VSL should be included in determining semen quality together with primary parameters for successful AI and high breeding performance in the swine industry.

No MeSH data available.


Related in: MedlinePlus

Comparison of motion and morphological index. Two motion indices were evaluated for boar 2004, 2012, and 2023. (A) straightness (STR, %) was calculated as the ratio of straight-line velocity (VSL) to average path velocity (VAP), and (B) linearity (LIN, %) was defined as the ratio of VSL to curvilinear velocity (VCL). Morphological abnormality was evaluated by measuring (C) sperm head elongation ratio (%) and (D) area (μm2). Elongation was calculated by the ratio of minor to major axis of sperm head, and area corresponded to the head size. Semen was collected at five different times from the boars (n = 5). The collected semen was divided in triplicates and applied to CASA analysis. The results are expressed as mean±SD. p<0.05. CASA, computer assisted semen analysis; SD, standard deviation.
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f3-ajas-27-10-1417: Comparison of motion and morphological index. Two motion indices were evaluated for boar 2004, 2012, and 2023. (A) straightness (STR, %) was calculated as the ratio of straight-line velocity (VSL) to average path velocity (VAP), and (B) linearity (LIN, %) was defined as the ratio of VSL to curvilinear velocity (VCL). Morphological abnormality was evaluated by measuring (C) sperm head elongation ratio (%) and (D) area (μm2). Elongation was calculated by the ratio of minor to major axis of sperm head, and area corresponded to the head size. Semen was collected at five different times from the boars (n = 5). The collected semen was divided in triplicates and applied to CASA analysis. The results are expressed as mean±SD. p<0.05. CASA, computer assisted semen analysis; SD, standard deviation.

Mentions: For a close inspection of detailed motion parameters, sperm progressiveness, VAP, VSL, and VCL from the three semen samples were analyzed. Percentage values of progressive sperm from boar 2004, 2012, and 2023 were 31.0±5.7%, 11.8±0.5%, and 27.8±9.0%, respectively, and the portion of progressive sperm from boar 2012 was significantly lower than that of boar 2004 and 2023 (Figure 2A). In addition, VAP and VSL of sperm from boar 2012 also showed a significant reduction compared to those of boar 2004 and 2023 (Figure 2B and C), although VCL did not show any significant difference (Figure 2D). Based on detailed motion parameter data, straightness and linearity index of sperm were calculated. As expected, sperm straightness and linearity from boar 2012 were significantly lower than those of boar 2004 and 2023 (Figure 3A and B). To identify the reason of low grade motion parameters in semen from boar 2012, morphological abnormal sperm parameters such as elongation rate and area of sperm head were observed. However, no significant difference among the three sperm samples was observed in sperm head morphologic index (Figure 3C and D).


Pig Spermatozoa Defect in Acrosome Formation Caused Poor Motion Parameters and Fertilization Failure through Artificial Insemination and In vitro Fertilization.

Lee WY, Lee R, Kim HC, Lee KH, Cui XS, Kim NH, Kim SH, Lee IJ, Uhm SJ, Yoon MJ, Song H - Asian-australas. J. Anim. Sci. (2014)

Comparison of motion and morphological index. Two motion indices were evaluated for boar 2004, 2012, and 2023. (A) straightness (STR, %) was calculated as the ratio of straight-line velocity (VSL) to average path velocity (VAP), and (B) linearity (LIN, %) was defined as the ratio of VSL to curvilinear velocity (VCL). Morphological abnormality was evaluated by measuring (C) sperm head elongation ratio (%) and (D) area (μm2). Elongation was calculated by the ratio of minor to major axis of sperm head, and area corresponded to the head size. Semen was collected at five different times from the boars (n = 5). The collected semen was divided in triplicates and applied to CASA analysis. The results are expressed as mean±SD. p<0.05. CASA, computer assisted semen analysis; SD, standard deviation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150174&req=5

f3-ajas-27-10-1417: Comparison of motion and morphological index. Two motion indices were evaluated for boar 2004, 2012, and 2023. (A) straightness (STR, %) was calculated as the ratio of straight-line velocity (VSL) to average path velocity (VAP), and (B) linearity (LIN, %) was defined as the ratio of VSL to curvilinear velocity (VCL). Morphological abnormality was evaluated by measuring (C) sperm head elongation ratio (%) and (D) area (μm2). Elongation was calculated by the ratio of minor to major axis of sperm head, and area corresponded to the head size. Semen was collected at five different times from the boars (n = 5). The collected semen was divided in triplicates and applied to CASA analysis. The results are expressed as mean±SD. p<0.05. CASA, computer assisted semen analysis; SD, standard deviation.
Mentions: For a close inspection of detailed motion parameters, sperm progressiveness, VAP, VSL, and VCL from the three semen samples were analyzed. Percentage values of progressive sperm from boar 2004, 2012, and 2023 were 31.0±5.7%, 11.8±0.5%, and 27.8±9.0%, respectively, and the portion of progressive sperm from boar 2012 was significantly lower than that of boar 2004 and 2023 (Figure 2A). In addition, VAP and VSL of sperm from boar 2012 also showed a significant reduction compared to those of boar 2004 and 2023 (Figure 2B and C), although VCL did not show any significant difference (Figure 2D). Based on detailed motion parameter data, straightness and linearity index of sperm were calculated. As expected, sperm straightness and linearity from boar 2012 were significantly lower than those of boar 2004 and 2023 (Figure 3A and B). To identify the reason of low grade motion parameters in semen from boar 2012, morphological abnormal sperm parameters such as elongation rate and area of sperm head were observed. However, no significant difference among the three sperm samples was observed in sperm head morphologic index (Figure 3C and D).

Bottom Line: Parameters for the selection of semen mainly include total sperm motility, concentration, and morphology.The present study was designed to compare the motion characteristics, fertilization ability using in vitro fertilization (IVF), and acrosome formation of the semen from boars having low (boar number 2012) and normal (boar number 2004 and 2023) breeding performances.In conclusion, the present study suggests that the acrosome staining and detailed motion characteristics such as progressiveness, VCL, and VSL should be included in determining semen quality together with primary parameters for successful AI and high breeding performance in the swine industry.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science, College of Agriculture, Chungbuk National University, Choung-ju 361-763, Korea .

ABSTRACT
The selection of morphologically normal spermatozoa is critical to obtain high breeding performances in boar breeding farms and artificial insemination (AI) centers. Parameters for the selection of semen mainly include total sperm motility, concentration, and morphology. However, these primary parameters are often not reliable for discriminating between normal and abnormal, non-fertilizable spermatozoa. The present study was designed to compare the motion characteristics, fertilization ability using in vitro fertilization (IVF), and acrosome formation of the semen from boars having low (boar number 2012) and normal (boar number 2004 and 2023) breeding performances. The ultimate goal was to identify additional simple and easy criteria for the selection of normal sperm. There was no significant difference between boar 2004 and boar 2023 sperm total motility in computer assisted sperm analysis. However, boar number 2012 semen presented a significantly reduced population of rapid moving spermatozoa and an increased population of slow moving spermatozoa compared to boar numbers 2004 and 2023. Analysis of detailed motion characteristics revealed that sperm from boar number 2012 had significantly reduced motility in progressiveness, average path velocity, straight-line velocity (VSL), curvilinear velocity (VCL), straightness, and linearity. The assessment of the fertilizing ability by IVF also showed that sperm from boar number 2012 showed a fertility rate of 3.4%, whereas sperm from boar number 2023 had a fertility rate of 75.45%. Interestingly, most of the sperm nuclei were found on the peripheral area of the oocytes, suggesting that the sperm from boar number 2012 lacked penetration ability into the oocyte zonapellucida. The acrosome formation analysis using Pisum sativum agglutinin staining demonstrated that the sperm from boar number 2012 had a defect in acrosome formation. Consequently, primary parameters for selecting semen before AI such as motility are not sufficient to select normal and fertilizable spermatozoa. In conclusion, the present study suggests that the acrosome staining and detailed motion characteristics such as progressiveness, VCL, and VSL should be included in determining semen quality together with primary parameters for successful AI and high breeding performance in the swine industry.

No MeSH data available.


Related in: MedlinePlus