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Cypermethrin-induced reproductive toxicity in the rat is prevented by resveratrol.

Sharma P, Huq AU, Singh R - J Hum Reprod Sci (2014)

Bottom Line: Cypermethrin exposure resulted in a significant decrease in weight of testis and epididymis, testicular sperm head counts, sperm motility and live sperm counts and increase in sperm abnormalities.Serum testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) and total protein (TP) content were decreased and lipid peroxidation (LPO) level was increased on cypermethrin exposure.The study concluded that resveratrol ameliorated cypermethrin-induced testicular damage by reducing oxidative stress and by enhancing the level of sex hormones.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Bundelkhand University, Jhansi, Uttar Pradesh, India.

ABSTRACT

Aims: The current study was to assess the protective role of resveratrol in cypermethrin-induced reproductive toxicity in male Wistar rats.

Materials and methods: Rats were exposed to cypermethrin (3.83 mg/kg bw) for 14 days. Pre- and post-treatment of resveratrol (20 mg/kg bw for 14 days) was given to cypermethrin exposed rats. At the end of the experiment, rats were sacrificed, testis and epididymis were removed, sperm characteristics, sex hormones, and various biochemical parameters were studied.

Results: Cypermethrin exposure resulted in a significant decrease in weight of testis and epididymis, testicular sperm head counts, sperm motility and live sperm counts and increase in sperm abnormalities. Serum testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) and total protein (TP) content were decreased and lipid peroxidation (LPO) level was increased on cypermethrin exposure. Pre- and post-treatment of resveratrol increased sperm head counts, sperm motility, live sperm counts, T, FSH, LH, GSH, CAT, SOD, GST, GR, GPx and TP contents and decreased LPO. Treatment with resveratrol alone has improved sperm parameters and testicular antioxidant defence system.

Conclusion: The study concluded that resveratrol ameliorated cypermethrin-induced testicular damage by reducing oxidative stress and by enhancing the level of sex hormones.

No MeSH data available.


Related in: MedlinePlus

Abnormal sperm tail and head morphology after cypermethrin exposure. a-Normal sperm, b-Headless tail, c-Bent tail, d-Coiled tail, e-Broken tail, f-Detached head
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Figure 3: Abnormal sperm tail and head morphology after cypermethrin exposure. a-Normal sperm, b-Headless tail, c-Bent tail, d-Coiled tail, e-Broken tail, f-Detached head

Mentions: The results showing an increase in cypermethrin-induced Sperm morphological abnormalities and protective role of resveratrol is summarized in the Table 2. Sperm abnormalities significantly increased in G-B (91%, P < 0.01). In post- and pre-treatment of resveratrol, the percentage of sperm morphological abnormalities was low (G-C, 44%, P < 0.05 and G-D, 35%, P < 0.05). However, sperm abnormalities were significantly (P < 0.01) reduced (26%) in G-E as compared to G-A. Increase in headless tail (7%), broken tail (1.5%), coiled tail (1.5%), bent tail (3%), detached head (3%) was observed in G-B. In G-C and D, headless tail (5, 4%), broken tail (1, 1%), coiled tail (1.5, 1%), bent tail (2, 2.5%) and detached head (2, 2.5%) have been reported [Table 3 and Figure 3]. All comparison were made with control G-A.


Cypermethrin-induced reproductive toxicity in the rat is prevented by resveratrol.

Sharma P, Huq AU, Singh R - J Hum Reprod Sci (2014)

Abnormal sperm tail and head morphology after cypermethrin exposure. a-Normal sperm, b-Headless tail, c-Bent tail, d-Coiled tail, e-Broken tail, f-Detached head
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150150&req=5

Figure 3: Abnormal sperm tail and head morphology after cypermethrin exposure. a-Normal sperm, b-Headless tail, c-Bent tail, d-Coiled tail, e-Broken tail, f-Detached head
Mentions: The results showing an increase in cypermethrin-induced Sperm morphological abnormalities and protective role of resveratrol is summarized in the Table 2. Sperm abnormalities significantly increased in G-B (91%, P < 0.01). In post- and pre-treatment of resveratrol, the percentage of sperm morphological abnormalities was low (G-C, 44%, P < 0.05 and G-D, 35%, P < 0.05). However, sperm abnormalities were significantly (P < 0.01) reduced (26%) in G-E as compared to G-A. Increase in headless tail (7%), broken tail (1.5%), coiled tail (1.5%), bent tail (3%), detached head (3%) was observed in G-B. In G-C and D, headless tail (5, 4%), broken tail (1, 1%), coiled tail (1.5, 1%), bent tail (2, 2.5%) and detached head (2, 2.5%) have been reported [Table 3 and Figure 3]. All comparison were made with control G-A.

Bottom Line: Cypermethrin exposure resulted in a significant decrease in weight of testis and epididymis, testicular sperm head counts, sperm motility and live sperm counts and increase in sperm abnormalities.Serum testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) and total protein (TP) content were decreased and lipid peroxidation (LPO) level was increased on cypermethrin exposure.The study concluded that resveratrol ameliorated cypermethrin-induced testicular damage by reducing oxidative stress and by enhancing the level of sex hormones.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Bundelkhand University, Jhansi, Uttar Pradesh, India.

ABSTRACT

Aims: The current study was to assess the protective role of resveratrol in cypermethrin-induced reproductive toxicity in male Wistar rats.

Materials and methods: Rats were exposed to cypermethrin (3.83 mg/kg bw) for 14 days. Pre- and post-treatment of resveratrol (20 mg/kg bw for 14 days) was given to cypermethrin exposed rats. At the end of the experiment, rats were sacrificed, testis and epididymis were removed, sperm characteristics, sex hormones, and various biochemical parameters were studied.

Results: Cypermethrin exposure resulted in a significant decrease in weight of testis and epididymis, testicular sperm head counts, sperm motility and live sperm counts and increase in sperm abnormalities. Serum testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) and total protein (TP) content were decreased and lipid peroxidation (LPO) level was increased on cypermethrin exposure. Pre- and post-treatment of resveratrol increased sperm head counts, sperm motility, live sperm counts, T, FSH, LH, GSH, CAT, SOD, GST, GR, GPx and TP contents and decreased LPO. Treatment with resveratrol alone has improved sperm parameters and testicular antioxidant defence system.

Conclusion: The study concluded that resveratrol ameliorated cypermethrin-induced testicular damage by reducing oxidative stress and by enhancing the level of sex hormones.

No MeSH data available.


Related in: MedlinePlus