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Cypermethrin-induced reproductive toxicity in the rat is prevented by resveratrol.

Sharma P, Huq AU, Singh R - J Hum Reprod Sci (2014)

Bottom Line: Cypermethrin exposure resulted in a significant decrease in weight of testis and epididymis, testicular sperm head counts, sperm motility and live sperm counts and increase in sperm abnormalities.Serum testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) and total protein (TP) content were decreased and lipid peroxidation (LPO) level was increased on cypermethrin exposure.The study concluded that resveratrol ameliorated cypermethrin-induced testicular damage by reducing oxidative stress and by enhancing the level of sex hormones.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Bundelkhand University, Jhansi, Uttar Pradesh, India.

ABSTRACT

Aims: The current study was to assess the protective role of resveratrol in cypermethrin-induced reproductive toxicity in male Wistar rats.

Materials and methods: Rats were exposed to cypermethrin (3.83 mg/kg bw) for 14 days. Pre- and post-treatment of resveratrol (20 mg/kg bw for 14 days) was given to cypermethrin exposed rats. At the end of the experiment, rats were sacrificed, testis and epididymis were removed, sperm characteristics, sex hormones, and various biochemical parameters were studied.

Results: Cypermethrin exposure resulted in a significant decrease in weight of testis and epididymis, testicular sperm head counts, sperm motility and live sperm counts and increase in sperm abnormalities. Serum testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) and total protein (TP) content were decreased and lipid peroxidation (LPO) level was increased on cypermethrin exposure. Pre- and post-treatment of resveratrol increased sperm head counts, sperm motility, live sperm counts, T, FSH, LH, GSH, CAT, SOD, GST, GR, GPx and TP contents and decreased LPO. Treatment with resveratrol alone has improved sperm parameters and testicular antioxidant defence system.

Conclusion: The study concluded that resveratrol ameliorated cypermethrin-induced testicular damage by reducing oxidative stress and by enhancing the level of sex hormones.

No MeSH data available.


Related in: MedlinePlus

Effect of cypermethrin and resveratrol on the weight of testis and epididymis. The values represent as Mean ± SEM for 6 rats each. c=P < 0.01 a=P > 0.05 as compared with control value. G-A=Control, G-B=Cypermethrin; G-C=Post-treatment group, G-D=Pre-treatment group and G-E=Resveratrol treatment groups
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Figure 1: Effect of cypermethrin and resveratrol on the weight of testis and epididymis. The values represent as Mean ± SEM for 6 rats each. c=P < 0.01 a=P > 0.05 as compared with control value. G-A=Control, G-B=Cypermethrin; G-C=Post-treatment group, G-D=Pre-treatment group and G-E=Resveratrol treatment groups

Mentions: Significant (P < 0.01) decrease in the weight of testis (11.66%) and epididymis (22.72%) was observed in G-B, indicating the deleterious effect of cypermethrin. In post- and pre-treatment Group C and D the decrease in testis weight was 4.13%, where as in epididymis it was 18.18 and 21.7%, respectively. The improvement in testis and epididymis weight in G-C and G-D compared with G-B suggested the ameliorating effect of resveratrol. The weight improvement was more pronounced in testis compared to epididymis. Nonsignificant (P > 0.05) increase in the weight of testis (2.47%) and significant (P < 0.05) increase in weight of epididymis (4.54%) was observed in G-E [Figure 1].


Cypermethrin-induced reproductive toxicity in the rat is prevented by resveratrol.

Sharma P, Huq AU, Singh R - J Hum Reprod Sci (2014)

Effect of cypermethrin and resveratrol on the weight of testis and epididymis. The values represent as Mean ± SEM for 6 rats each. c=P < 0.01 a=P > 0.05 as compared with control value. G-A=Control, G-B=Cypermethrin; G-C=Post-treatment group, G-D=Pre-treatment group and G-E=Resveratrol treatment groups
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150150&req=5

Figure 1: Effect of cypermethrin and resveratrol on the weight of testis and epididymis. The values represent as Mean ± SEM for 6 rats each. c=P < 0.01 a=P > 0.05 as compared with control value. G-A=Control, G-B=Cypermethrin; G-C=Post-treatment group, G-D=Pre-treatment group and G-E=Resveratrol treatment groups
Mentions: Significant (P < 0.01) decrease in the weight of testis (11.66%) and epididymis (22.72%) was observed in G-B, indicating the deleterious effect of cypermethrin. In post- and pre-treatment Group C and D the decrease in testis weight was 4.13%, where as in epididymis it was 18.18 and 21.7%, respectively. The improvement in testis and epididymis weight in G-C and G-D compared with G-B suggested the ameliorating effect of resveratrol. The weight improvement was more pronounced in testis compared to epididymis. Nonsignificant (P > 0.05) increase in the weight of testis (2.47%) and significant (P < 0.05) increase in weight of epididymis (4.54%) was observed in G-E [Figure 1].

Bottom Line: Cypermethrin exposure resulted in a significant decrease in weight of testis and epididymis, testicular sperm head counts, sperm motility and live sperm counts and increase in sperm abnormalities.Serum testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) and total protein (TP) content were decreased and lipid peroxidation (LPO) level was increased on cypermethrin exposure.The study concluded that resveratrol ameliorated cypermethrin-induced testicular damage by reducing oxidative stress and by enhancing the level of sex hormones.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Bundelkhand University, Jhansi, Uttar Pradesh, India.

ABSTRACT

Aims: The current study was to assess the protective role of resveratrol in cypermethrin-induced reproductive toxicity in male Wistar rats.

Materials and methods: Rats were exposed to cypermethrin (3.83 mg/kg bw) for 14 days. Pre- and post-treatment of resveratrol (20 mg/kg bw for 14 days) was given to cypermethrin exposed rats. At the end of the experiment, rats were sacrificed, testis and epididymis were removed, sperm characteristics, sex hormones, and various biochemical parameters were studied.

Results: Cypermethrin exposure resulted in a significant decrease in weight of testis and epididymis, testicular sperm head counts, sperm motility and live sperm counts and increase in sperm abnormalities. Serum testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) and total protein (TP) content were decreased and lipid peroxidation (LPO) level was increased on cypermethrin exposure. Pre- and post-treatment of resveratrol increased sperm head counts, sperm motility, live sperm counts, T, FSH, LH, GSH, CAT, SOD, GST, GR, GPx and TP contents and decreased LPO. Treatment with resveratrol alone has improved sperm parameters and testicular antioxidant defence system.

Conclusion: The study concluded that resveratrol ameliorated cypermethrin-induced testicular damage by reducing oxidative stress and by enhancing the level of sex hormones.

No MeSH data available.


Related in: MedlinePlus