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Prevalence of abnormal spermatozoa in tobacco chewing sub-fertile males.

Sunanda P, Panda B, Dash C, Ray PK, Padhy RN, Routray P - J Hum Reprod Sci (2014)

Bottom Line: The aim of the following study is to find out the prevalence of abnormal spermatozoa and associated functional parameters in clinical semen samples of sub-fertile males with the tobacco chewing habit.Sperm counts (odds ratio [OR] =2.2; 95% confidence interval [CI]: 1.5-3.09), motility (OR = 3.2; 95% CI: 2.05-4.9), and normal morphology (OR = 8.4; 95% CI: 4.9-14.6) were significantly affected (P = 0.001) in tobacco chewers than the non-chewing group.Structural defects in head (P = 0.001) and cytoplasmic residues (P = 0.001) were found to be positively correlated with the intensive chewing, but no significant changes were found in anomalies in mid-piece and tail.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Center for Human Reproduction, Institute of Medical Sciences and SUM Hospital, Bhubaneswar, Odisha, India.

ABSTRACT

Aim: The aim of the following study is to find out the prevalence of abnormal spermatozoa and associated functional parameters in clinical semen samples of sub-fertile males with the tobacco chewing habit.

Settings and design: Retrospective study was conducted at infertility unit of a tertiary health care center, in a period of 3 years.

Materials and method: Semen of 642 males were analyzed; of them 194 men (30.2%) were tobacco chewers and they were grouped according to their intensity of chewing (<10 and ≥ 10 packets/day). Counts, motility, vitality, and morphology of sperms were analyzed.

Results: In tobacco chewers, 66% of subjects were oligozoospermic, 85% asthenozoospermic and 28% teratozoospermic. Sperm counts (odds ratio [OR] =2.2; 95% confidence interval [CI]: 1.5-3.09), motility (OR = 3.2; 95% CI: 2.05-4.9), and normal morphology (OR = 8.4; 95% CI: 4.9-14.6) were significantly affected (P = 0.001) in tobacco chewers than the non-chewing group. Further, in comparison to the intensity of tobacco chewing, patients with the intensive practice of using ≥10 packets/day had a significant effect on sperm morphology (P = 0.003, OR = 2.7; 95% CI = 1.41-5.08) only. Structural defects in head (P = 0.001) and cytoplasmic residues (P = 0.001) were found to be positively correlated with the intensive chewing, but no significant changes were found in anomalies in mid-piece and tail.

Conclusion: The adverse impact of tobacco chewing on semen parameters was evident even with mild chewers, but with the intensive chewing practice, phenotypes of sperms, mainly defects in the head and cytoplasmic residue were severely affected.

No MeSH data available.


Related in: MedlinePlus

Amplitude images of sperm by atomic force microscopy. The two-dimensional images represent length and width in X and Y-axis, respectively. Each small division represents 2 μ: (a) Abnormal acrosome; (b) Abnormal mid-piece; (c) Bent neck with irregular acrosome; and (d) Double head with abnormal mid-piece
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Figure 2: Amplitude images of sperm by atomic force microscopy. The two-dimensional images represent length and width in X and Y-axis, respectively. Each small division represents 2 μ: (a) Abnormal acrosome; (b) Abnormal mid-piece; (c) Bent neck with irregular acrosome; and (d) Double head with abnormal mid-piece

Mentions: As OR value represented 8.4 times higher to be of teratozoospermics in tobacco chewers than non-chewers, this study intensified specific morphological anomalies due to tobacco chewing [Table 4]. Deciphering the sperm morphology, many abnormal features were observed in head, mid-piece and tail [Figure 1]. Double head, pin head, tapered head, bent neck, curved tail and tailless sperms were the several anomalous features abundantly marked. An initial attempt for sperm morphological study by AFM also showed significant abnormal features among tobacco chewers [Figure 2]. However, mean of head defects and cytoplasmic residues were higher in the sub-group IA having an increasing trend from the control group to intensive chewers.


Prevalence of abnormal spermatozoa in tobacco chewing sub-fertile males.

Sunanda P, Panda B, Dash C, Ray PK, Padhy RN, Routray P - J Hum Reprod Sci (2014)

Amplitude images of sperm by atomic force microscopy. The two-dimensional images represent length and width in X and Y-axis, respectively. Each small division represents 2 μ: (a) Abnormal acrosome; (b) Abnormal mid-piece; (c) Bent neck with irregular acrosome; and (d) Double head with abnormal mid-piece
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150141&req=5

Figure 2: Amplitude images of sperm by atomic force microscopy. The two-dimensional images represent length and width in X and Y-axis, respectively. Each small division represents 2 μ: (a) Abnormal acrosome; (b) Abnormal mid-piece; (c) Bent neck with irregular acrosome; and (d) Double head with abnormal mid-piece
Mentions: As OR value represented 8.4 times higher to be of teratozoospermics in tobacco chewers than non-chewers, this study intensified specific morphological anomalies due to tobacco chewing [Table 4]. Deciphering the sperm morphology, many abnormal features were observed in head, mid-piece and tail [Figure 1]. Double head, pin head, tapered head, bent neck, curved tail and tailless sperms were the several anomalous features abundantly marked. An initial attempt for sperm morphological study by AFM also showed significant abnormal features among tobacco chewers [Figure 2]. However, mean of head defects and cytoplasmic residues were higher in the sub-group IA having an increasing trend from the control group to intensive chewers.

Bottom Line: The aim of the following study is to find out the prevalence of abnormal spermatozoa and associated functional parameters in clinical semen samples of sub-fertile males with the tobacco chewing habit.Sperm counts (odds ratio [OR] =2.2; 95% confidence interval [CI]: 1.5-3.09), motility (OR = 3.2; 95% CI: 2.05-4.9), and normal morphology (OR = 8.4; 95% CI: 4.9-14.6) were significantly affected (P = 0.001) in tobacco chewers than the non-chewing group.Structural defects in head (P = 0.001) and cytoplasmic residues (P = 0.001) were found to be positively correlated with the intensive chewing, but no significant changes were found in anomalies in mid-piece and tail.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Center for Human Reproduction, Institute of Medical Sciences and SUM Hospital, Bhubaneswar, Odisha, India.

ABSTRACT

Aim: The aim of the following study is to find out the prevalence of abnormal spermatozoa and associated functional parameters in clinical semen samples of sub-fertile males with the tobacco chewing habit.

Settings and design: Retrospective study was conducted at infertility unit of a tertiary health care center, in a period of 3 years.

Materials and method: Semen of 642 males were analyzed; of them 194 men (30.2%) were tobacco chewers and they were grouped according to their intensity of chewing (<10 and ≥ 10 packets/day). Counts, motility, vitality, and morphology of sperms were analyzed.

Results: In tobacco chewers, 66% of subjects were oligozoospermic, 85% asthenozoospermic and 28% teratozoospermic. Sperm counts (odds ratio [OR] =2.2; 95% confidence interval [CI]: 1.5-3.09), motility (OR = 3.2; 95% CI: 2.05-4.9), and normal morphology (OR = 8.4; 95% CI: 4.9-14.6) were significantly affected (P = 0.001) in tobacco chewers than the non-chewing group. Further, in comparison to the intensity of tobacco chewing, patients with the intensive practice of using ≥10 packets/day had a significant effect on sperm morphology (P = 0.003, OR = 2.7; 95% CI = 1.41-5.08) only. Structural defects in head (P = 0.001) and cytoplasmic residues (P = 0.001) were found to be positively correlated with the intensive chewing, but no significant changes were found in anomalies in mid-piece and tail.

Conclusion: The adverse impact of tobacco chewing on semen parameters was evident even with mild chewers, but with the intensive chewing practice, phenotypes of sperms, mainly defects in the head and cytoplasmic residue were severely affected.

No MeSH data available.


Related in: MedlinePlus