Nuclear envelope protein MAN1 regulates clock through BMAL1.
Bottom Line: Our knowledge of these components and pathways is far from exhaustive.In recent decades, the nuclear envelope has emerged as a global gene regulatory machine, although its role in circadian regulation has not been explored.Our results establish a novel connection between the nuclear periphery and circadian rhythmicity, therefore bridging two global regulatory systems that modulate all aspects of bodily functions.
Affiliation: Department of Neurology, University of California, San Francisco, San Francisco, United States.Show MeSH
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Mentions: Since MAN1 does not execute its function through RORE, we investigated the promoter region of BMAL1 to determine what is necessary for the enhancing effect of MAN1. A series of deletion constructs of the BMAL1 promoter were generated for luciferase assays and a 900 bp region (−795 ∼ +106) was identified to be the region harboring the necessary DNA sequence for the regulatory effect of MAN1 on BMAL1 (Figure 8A, Figure 8—figure supplement 1A).10.7554/eLife.02981.018Figure 8.MAN1 binds to the BMAL1 promoter to enhance its transcription.
Affiliation: Department of Neurology, University of California, San Francisco, San Francisco, United States.