Nuclear envelope protein MAN1 regulates clock through BMAL1.
Bottom Line: Our knowledge of these components and pathways is far from exhaustive.In recent decades, the nuclear envelope has emerged as a global gene regulatory machine, although its role in circadian regulation has not been explored.Our results establish a novel connection between the nuclear periphery and circadian rhythmicity, therefore bridging two global regulatory systems that modulate all aspects of bodily functions.
Affiliation: Department of Neurology, University of California, San Francisco, San Francisco, United States.Show MeSH
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Mentions: Consistent with the cell culture data, over-expressing MAN1 in all clock cells in flies using a timeless (tim) GAL4 driver (Emery et al., 1998) resulted in a significantly increased level of cycle (cyc) mRNA, the Drosophila BMAL1 homologue (Rutila et al., 1998; Figure 5A). The mRNA level of core clock gene tim was also significantly elevated. In addition, we have assessed the levels of MAN1 mRNA to confirm over-expression (Figure 5B). We also examined the effect of knocking down MAN1 in clock cells but did not observe altered cyc mRNA levels (Figure 5—figure supplement 1).10.7554/eLife.02981.014Figure 5.MAN1 increases cyc mRNA levels.
Affiliation: Department of Neurology, University of California, San Francisco, San Francisco, United States.