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Nuclear envelope protein MAN1 regulates clock through BMAL1.

Lin ST, Zhang L, Lin X, Zhang LC, Garcia VE, Tsai CW, Ptáček L, Fu YH - Elife (2014)

Bottom Line: Our knowledge of these components and pathways is far from exhaustive.In recent decades, the nuclear envelope has emerged as a global gene regulatory machine, although its role in circadian regulation has not been explored.Our results establish a novel connection between the nuclear periphery and circadian rhythmicity, therefore bridging two global regulatory systems that modulate all aspects of bodily functions.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, University of California, San Francisco, San Francisco, United States.

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Related in: MedlinePlus

cyc transcript level is not altered in MAN1 knock-down flies.Plot of relative mRNA abundance for cyc from whole head extracts of timGAL4/+;UASdicer2/+ and timGAL4/UASMAN1RNAi;UASdicer2/+ flies determined by qRT-PCR. Error bars represent SEM (n = 6). The value of timGAL4/+;UASdicer2/+ for one experiment was set to 1.DOI:http://dx.doi.org/10.7554/eLife.02981.015
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fig5s1: cyc transcript level is not altered in MAN1 knock-down flies.Plot of relative mRNA abundance for cyc from whole head extracts of timGAL4/+;UASdicer2/+ and timGAL4/UASMAN1RNAi;UASdicer2/+ flies determined by qRT-PCR. Error bars represent SEM (n = 6). The value of timGAL4/+;UASdicer2/+ for one experiment was set to 1.DOI:http://dx.doi.org/10.7554/eLife.02981.015

Mentions: Consistent with the cell culture data, over-expressing MAN1 in all clock cells in flies using a timeless (tim) GAL4 driver (Emery et al., 1998) resulted in a significantly increased level of cycle (cyc) mRNA, the Drosophila BMAL1 homologue (Rutila et al., 1998; Figure 5A). The mRNA level of core clock gene tim was also significantly elevated. In addition, we have assessed the levels of MAN1 mRNA to confirm over-expression (Figure 5B). We also examined the effect of knocking down MAN1 in clock cells but did not observe altered cyc mRNA levels (Figure 5—figure supplement 1).10.7554/eLife.02981.014Figure 5.MAN1 increases cyc mRNA levels.


Nuclear envelope protein MAN1 regulates clock through BMAL1.

Lin ST, Zhang L, Lin X, Zhang LC, Garcia VE, Tsai CW, Ptáček L, Fu YH - Elife (2014)

cyc transcript level is not altered in MAN1 knock-down flies.Plot of relative mRNA abundance for cyc from whole head extracts of timGAL4/+;UASdicer2/+ and timGAL4/UASMAN1RNAi;UASdicer2/+ flies determined by qRT-PCR. Error bars represent SEM (n = 6). The value of timGAL4/+;UASdicer2/+ for one experiment was set to 1.DOI:http://dx.doi.org/10.7554/eLife.02981.015
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4150126&req=5

fig5s1: cyc transcript level is not altered in MAN1 knock-down flies.Plot of relative mRNA abundance for cyc from whole head extracts of timGAL4/+;UASdicer2/+ and timGAL4/UASMAN1RNAi;UASdicer2/+ flies determined by qRT-PCR. Error bars represent SEM (n = 6). The value of timGAL4/+;UASdicer2/+ for one experiment was set to 1.DOI:http://dx.doi.org/10.7554/eLife.02981.015
Mentions: Consistent with the cell culture data, over-expressing MAN1 in all clock cells in flies using a timeless (tim) GAL4 driver (Emery et al., 1998) resulted in a significantly increased level of cycle (cyc) mRNA, the Drosophila BMAL1 homologue (Rutila et al., 1998; Figure 5A). The mRNA level of core clock gene tim was also significantly elevated. In addition, we have assessed the levels of MAN1 mRNA to confirm over-expression (Figure 5B). We also examined the effect of knocking down MAN1 in clock cells but did not observe altered cyc mRNA levels (Figure 5—figure supplement 1).10.7554/eLife.02981.014Figure 5.MAN1 increases cyc mRNA levels.

Bottom Line: Our knowledge of these components and pathways is far from exhaustive.In recent decades, the nuclear envelope has emerged as a global gene regulatory machine, although its role in circadian regulation has not been explored.Our results establish a novel connection between the nuclear periphery and circadian rhythmicity, therefore bridging two global regulatory systems that modulate all aspects of bodily functions.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, University of California, San Francisco, San Francisco, United States.

Show MeSH
Related in: MedlinePlus