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Importance of the association of molecular and immunological diagnosis in immunocompetent patient with Histoplasma capsulatum and Cryptoccocus neoformans infection: a case report.

Dantas KC, de Freitas RS, Garcia RS, da Silva MV, Muricy EC, Kohara VS, Vicentini AP - J Venom Anim Toxins Incl Trop Dis (2014)

Bottom Line: Nested-PCR in blood employing species-specific primers was positive for H. capsulatum and Cryptococcus neoformans.The evaluation of paucisymptomatic patients with positive results for H. capsulatum and C. neoformans could be relevant for the prevention as well as the possible evaluation of the reactivated quiescent foci.In conclusion, the associated methodology may have contributed to the monitoring endogenous reactivation of these diseases.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Medical School, University of São Paulo, São Paulo, São Paulo State, Brazil.

ABSTRACT
This case reports an immunocompetent 29-year-old woman with suspected pneumonia, suggestive of fungal infection. Immunoblotting analysis reactivity against Histoplasma capsulatum and Paracoccidioides brasiliensis were observed. Nested-PCR in blood employing species-specific primers was positive for H. capsulatum and Cryptococcus neoformans. The evaluation of paucisymptomatic patients with positive results for H. capsulatum and C. neoformans could be relevant for the prevention as well as the possible evaluation of the reactivated quiescent foci. In conclusion, the associated methodology may have contributed to the monitoring endogenous reactivation of these diseases.

No MeSH data available.


Related in: MedlinePlus

Immunoblotting and PCR assays. (A): Immunoreactivity of serum patient against H and M fractions of H. capsulatum. (B): Lane M. DNA molecular weight marker 1kb DNA ladder; Lane 1. patient serum collected in February 2008; Lane 2.- patient serum collected in June 2008; Lanes 3 to 10. negative individual sera samples, Lane Hc1. H. capsulatum strain (immunodeficiency); Lane Hc2. H. capsulatum 200 strain; Lane Pb 18. P. brasiliensis strain; Lane Pb 339 P. brasiliensis 339, Lane CN1 e CN2. C. neoformans strains (ATCC 24067) and Lane NO. negative control.
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Figure 1: Immunoblotting and PCR assays. (A): Immunoreactivity of serum patient against H and M fractions of H. capsulatum. (B): Lane M. DNA molecular weight marker 1kb DNA ladder; Lane 1. patient serum collected in February 2008; Lane 2.- patient serum collected in June 2008; Lanes 3 to 10. negative individual sera samples, Lane Hc1. H. capsulatum strain (immunodeficiency); Lane Hc2. H. capsulatum 200 strain; Lane Pb 18. P. brasiliensis strain; Lane Pb 339 P. brasiliensis 339, Lane CN1 e CN2. C. neoformans strains (ATCC 24067) and Lane NO. negative control.

Mentions: Katzenstein [33] studied specificity of skin tests in deep fungal infection, showing that the response to skin testing is closely related to the concentration of antigen employed. In this paper, the author confirms the anergic state, since the reaction of delayed-type hypersensitivity showed no response. Infection by H. capsulatum was later confirmed at autopsy [33]. The reaction of nested PCR was positive for H. capsulatum and C. neoformans, and negative for P. brasiliensis, showing the possible infection by H. capsulatum observed in immunoblotting (Figure 1A).


Importance of the association of molecular and immunological diagnosis in immunocompetent patient with Histoplasma capsulatum and Cryptoccocus neoformans infection: a case report.

Dantas KC, de Freitas RS, Garcia RS, da Silva MV, Muricy EC, Kohara VS, Vicentini AP - J Venom Anim Toxins Incl Trop Dis (2014)

Immunoblotting and PCR assays. (A): Immunoreactivity of serum patient against H and M fractions of H. capsulatum. (B): Lane M. DNA molecular weight marker 1kb DNA ladder; Lane 1. patient serum collected in February 2008; Lane 2.- patient serum collected in June 2008; Lanes 3 to 10. negative individual sera samples, Lane Hc1. H. capsulatum strain (immunodeficiency); Lane Hc2. H. capsulatum 200 strain; Lane Pb 18. P. brasiliensis strain; Lane Pb 339 P. brasiliensis 339, Lane CN1 e CN2. C. neoformans strains (ATCC 24067) and Lane NO. negative control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4150115&req=5

Figure 1: Immunoblotting and PCR assays. (A): Immunoreactivity of serum patient against H and M fractions of H. capsulatum. (B): Lane M. DNA molecular weight marker 1kb DNA ladder; Lane 1. patient serum collected in February 2008; Lane 2.- patient serum collected in June 2008; Lanes 3 to 10. negative individual sera samples, Lane Hc1. H. capsulatum strain (immunodeficiency); Lane Hc2. H. capsulatum 200 strain; Lane Pb 18. P. brasiliensis strain; Lane Pb 339 P. brasiliensis 339, Lane CN1 e CN2. C. neoformans strains (ATCC 24067) and Lane NO. negative control.
Mentions: Katzenstein [33] studied specificity of skin tests in deep fungal infection, showing that the response to skin testing is closely related to the concentration of antigen employed. In this paper, the author confirms the anergic state, since the reaction of delayed-type hypersensitivity showed no response. Infection by H. capsulatum was later confirmed at autopsy [33]. The reaction of nested PCR was positive for H. capsulatum and C. neoformans, and negative for P. brasiliensis, showing the possible infection by H. capsulatum observed in immunoblotting (Figure 1A).

Bottom Line: Nested-PCR in blood employing species-specific primers was positive for H. capsulatum and Cryptococcus neoformans.The evaluation of paucisymptomatic patients with positive results for H. capsulatum and C. neoformans could be relevant for the prevention as well as the possible evaluation of the reactivated quiescent foci.In conclusion, the associated methodology may have contributed to the monitoring endogenous reactivation of these diseases.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Medical School, University of São Paulo, São Paulo, São Paulo State, Brazil.

ABSTRACT
This case reports an immunocompetent 29-year-old woman with suspected pneumonia, suggestive of fungal infection. Immunoblotting analysis reactivity against Histoplasma capsulatum and Paracoccidioides brasiliensis were observed. Nested-PCR in blood employing species-specific primers was positive for H. capsulatum and Cryptococcus neoformans. The evaluation of paucisymptomatic patients with positive results for H. capsulatum and C. neoformans could be relevant for the prevention as well as the possible evaluation of the reactivated quiescent foci. In conclusion, the associated methodology may have contributed to the monitoring endogenous reactivation of these diseases.

No MeSH data available.


Related in: MedlinePlus