Limits...
Species limits in Diaporthe: molecular re-assessment of D. citri, D. cytosporella, D. foeniculina and D. rudis.

Udayanga D, Castlebury LA, Rossman AY, Hyde KD - Persoonia (2014)

Bottom Line: Diaporthe cytosporella is found on Citrus in Europe and California (USA).Newly designed primers are introduced to optimise the amplification and sequencing of calmodulin and actin genes in Diaporthe.A discussion is provided of the utility of genes and the need for multi-gene phylogenies when distinguishing species of Diaporthe or describing new species.

View Article: PubMed Central - PubMed

Affiliation: Systematic Mycology and Microbiology Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, 20705 USA; ; Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. ; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.

ABSTRACT
Species of Diaporthe are important plant pathogens of a wide range of hosts worldwide. In the present study the species causing melanose and stem end rot diseases of Citrus spp. are revised. Three species of Diaporthe occurring on Citrus are characterised, including D. citri, D. cytosporella and D. foeniculina. Morphology and phylogenetic analyses of the complete nuclear ribosomal internal transcribed spacer regions and partial sequences of actin, beta-tubulin, calmodulin and translation elongation factor 1-α were used to resolve species on Citrus and related Diaporthe species. Diaporthe citri occurs on Citrus throughout the Citrus-growing regions of the world. Diaporthe cytosporella is found on Citrus in Europe and California (USA). Diaporthe foeniculina, including the synonym D. neotheicola, is recognised as a species with an extensive host range including Citrus. Diaporthe medusaea, a name widely used for D. citri, was determined to be a synonym of D. rudis, a species with a broad host range. Diaporthe citri is delimited based on molecular phylogenetic analysis with the inclusion of the conserved ex-type and additional collections from different geographic locations worldwide. Diaporthe cytosporella, D. foeniculina and D. rudis are epitypified, fully described and illustrated with a review of all synonyms based on molecular data and morphological studies. Newly designed primers are introduced to optimise the amplification and sequencing of calmodulin and actin genes in Diaporthe. A discussion is provided of the utility of genes and the need for multi-gene phylogenies when distinguishing species of Diaporthe or describing new species.

No MeSH data available.


Related in: MedlinePlus

Diaporthe rudis. a, b. Ectostroma and perithecia on Laburnum anagyroides; c. perithecia in transverse section; d. perithecial wall in longitudinal section; e, f. perithecia in longitudinal section; g–j. asci; k. ascospores; l. conidiophores developing on alfalfa stem in culture; m. alpha and beta conidia developing on alfalfa stem in culture (a–k. Epitype specimen BPI 748231; l–n. ex-epitype culture AR3422 = CBS 109292). — Scale bars: a = 2 000 μm; b, c = 1 000 μm; d = 50 μm; e, f = 100 μm; g–k = 25 μm; l–n = 15 μm.
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Figure 8: Diaporthe rudis. a, b. Ectostroma and perithecia on Laburnum anagyroides; c. perithecia in transverse section; d. perithecial wall in longitudinal section; e, f. perithecia in longitudinal section; g–j. asci; k. ascospores; l. conidiophores developing on alfalfa stem in culture; m. alpha and beta conidia developing on alfalfa stem in culture (a–k. Epitype specimen BPI 748231; l–n. ex-epitype culture AR3422 = CBS 109292). — Scale bars: a = 2 000 μm; b, c = 1 000 μm; d = 50 μm; e, f = 100 μm; g–k = 25 μm; l–n = 15 μm.

Mentions: Diaporthe rudis (Fr.) Nitschke, Pyrenomycetes Germanici 2: 282. 1870. — Fig. 8


Species limits in Diaporthe: molecular re-assessment of D. citri, D. cytosporella, D. foeniculina and D. rudis.

Udayanga D, Castlebury LA, Rossman AY, Hyde KD - Persoonia (2014)

Diaporthe rudis. a, b. Ectostroma and perithecia on Laburnum anagyroides; c. perithecia in transverse section; d. perithecial wall in longitudinal section; e, f. perithecia in longitudinal section; g–j. asci; k. ascospores; l. conidiophores developing on alfalfa stem in culture; m. alpha and beta conidia developing on alfalfa stem in culture (a–k. Epitype specimen BPI 748231; l–n. ex-epitype culture AR3422 = CBS 109292). — Scale bars: a = 2 000 μm; b, c = 1 000 μm; d = 50 μm; e, f = 100 μm; g–k = 25 μm; l–n = 15 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150081&req=5

Figure 8: Diaporthe rudis. a, b. Ectostroma and perithecia on Laburnum anagyroides; c. perithecia in transverse section; d. perithecial wall in longitudinal section; e, f. perithecia in longitudinal section; g–j. asci; k. ascospores; l. conidiophores developing on alfalfa stem in culture; m. alpha and beta conidia developing on alfalfa stem in culture (a–k. Epitype specimen BPI 748231; l–n. ex-epitype culture AR3422 = CBS 109292). — Scale bars: a = 2 000 μm; b, c = 1 000 μm; d = 50 μm; e, f = 100 μm; g–k = 25 μm; l–n = 15 μm.
Mentions: Diaporthe rudis (Fr.) Nitschke, Pyrenomycetes Germanici 2: 282. 1870. — Fig. 8

Bottom Line: Diaporthe cytosporella is found on Citrus in Europe and California (USA).Newly designed primers are introduced to optimise the amplification and sequencing of calmodulin and actin genes in Diaporthe.A discussion is provided of the utility of genes and the need for multi-gene phylogenies when distinguishing species of Diaporthe or describing new species.

View Article: PubMed Central - PubMed

Affiliation: Systematic Mycology and Microbiology Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, 20705 USA; ; Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. ; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.

ABSTRACT
Species of Diaporthe are important plant pathogens of a wide range of hosts worldwide. In the present study the species causing melanose and stem end rot diseases of Citrus spp. are revised. Three species of Diaporthe occurring on Citrus are characterised, including D. citri, D. cytosporella and D. foeniculina. Morphology and phylogenetic analyses of the complete nuclear ribosomal internal transcribed spacer regions and partial sequences of actin, beta-tubulin, calmodulin and translation elongation factor 1-α were used to resolve species on Citrus and related Diaporthe species. Diaporthe citri occurs on Citrus throughout the Citrus-growing regions of the world. Diaporthe cytosporella is found on Citrus in Europe and California (USA). Diaporthe foeniculina, including the synonym D. neotheicola, is recognised as a species with an extensive host range including Citrus. Diaporthe medusaea, a name widely used for D. citri, was determined to be a synonym of D. rudis, a species with a broad host range. Diaporthe citri is delimited based on molecular phylogenetic analysis with the inclusion of the conserved ex-type and additional collections from different geographic locations worldwide. Diaporthe cytosporella, D. foeniculina and D. rudis are epitypified, fully described and illustrated with a review of all synonyms based on molecular data and morphological studies. Newly designed primers are introduced to optimise the amplification and sequencing of calmodulin and actin genes in Diaporthe. A discussion is provided of the utility of genes and the need for multi-gene phylogenies when distinguishing species of Diaporthe or describing new species.

No MeSH data available.


Related in: MedlinePlus