Limits...
Species limits in Diaporthe: molecular re-assessment of D. citri, D. cytosporella, D. foeniculina and D. rudis.

Udayanga D, Castlebury LA, Rossman AY, Hyde KD - Persoonia (2014)

Bottom Line: Diaporthe cytosporella is found on Citrus in Europe and California (USA).Newly designed primers are introduced to optimise the amplification and sequencing of calmodulin and actin genes in Diaporthe.A discussion is provided of the utility of genes and the need for multi-gene phylogenies when distinguishing species of Diaporthe or describing new species.

View Article: PubMed Central - PubMed

Affiliation: Systematic Mycology and Microbiology Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, 20705 USA; ; Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. ; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.

ABSTRACT
Species of Diaporthe are important plant pathogens of a wide range of hosts worldwide. In the present study the species causing melanose and stem end rot diseases of Citrus spp. are revised. Three species of Diaporthe occurring on Citrus are characterised, including D. citri, D. cytosporella and D. foeniculina. Morphology and phylogenetic analyses of the complete nuclear ribosomal internal transcribed spacer regions and partial sequences of actin, beta-tubulin, calmodulin and translation elongation factor 1-α were used to resolve species on Citrus and related Diaporthe species. Diaporthe citri occurs on Citrus throughout the Citrus-growing regions of the world. Diaporthe cytosporella is found on Citrus in Europe and California (USA). Diaporthe foeniculina, including the synonym D. neotheicola, is recognised as a species with an extensive host range including Citrus. Diaporthe medusaea, a name widely used for D. citri, was determined to be a synonym of D. rudis, a species with a broad host range. Diaporthe citri is delimited based on molecular phylogenetic analysis with the inclusion of the conserved ex-type and additional collections from different geographic locations worldwide. Diaporthe cytosporella, D. foeniculina and D. rudis are epitypified, fully described and illustrated with a review of all synonyms based on molecular data and morphological studies. Newly designed primers are introduced to optimise the amplification and sequencing of calmodulin and actin genes in Diaporthe. A discussion is provided of the utility of genes and the need for multi-gene phylogenies when distinguishing species of Diaporthe or describing new species.

No MeSH data available.


Related in: MedlinePlus

Diaporthe cytosporella (AR5149). a. Sporulating pycnidia on alfalfa stem; b. culture on PDA (25 °C, dark, 7 d); c. concentric pycnidial in rings on culture; d. pycnidia on culture; e. conidiophores; f. alpha conidia. — Scale bars: a, c = 2 000 μm; d = 3 000 μm; e, f = 20 μm.
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Figure 5: Diaporthe cytosporella (AR5149). a. Sporulating pycnidia on alfalfa stem; b. culture on PDA (25 °C, dark, 7 d); c. concentric pycnidial in rings on culture; d. pycnidia on culture; e. conidiophores; f. alpha conidia. — Scale bars: a, c = 2 000 μm; d = 3 000 μm; e, f = 20 μm.

Mentions: Diaporthe cytosporella (Penz. & Sacc.) Udayanga & Castl., comb. nov. — MycoBank MB803986; Fig. 5, 6


Species limits in Diaporthe: molecular re-assessment of D. citri, D. cytosporella, D. foeniculina and D. rudis.

Udayanga D, Castlebury LA, Rossman AY, Hyde KD - Persoonia (2014)

Diaporthe cytosporella (AR5149). a. Sporulating pycnidia on alfalfa stem; b. culture on PDA (25 °C, dark, 7 d); c. concentric pycnidial in rings on culture; d. pycnidia on culture; e. conidiophores; f. alpha conidia. — Scale bars: a, c = 2 000 μm; d = 3 000 μm; e, f = 20 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150081&req=5

Figure 5: Diaporthe cytosporella (AR5149). a. Sporulating pycnidia on alfalfa stem; b. culture on PDA (25 °C, dark, 7 d); c. concentric pycnidial in rings on culture; d. pycnidia on culture; e. conidiophores; f. alpha conidia. — Scale bars: a, c = 2 000 μm; d = 3 000 μm; e, f = 20 μm.
Mentions: Diaporthe cytosporella (Penz. & Sacc.) Udayanga & Castl., comb. nov. — MycoBank MB803986; Fig. 5, 6

Bottom Line: Diaporthe cytosporella is found on Citrus in Europe and California (USA).Newly designed primers are introduced to optimise the amplification and sequencing of calmodulin and actin genes in Diaporthe.A discussion is provided of the utility of genes and the need for multi-gene phylogenies when distinguishing species of Diaporthe or describing new species.

View Article: PubMed Central - PubMed

Affiliation: Systematic Mycology and Microbiology Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, 20705 USA; ; Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. ; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.

ABSTRACT
Species of Diaporthe are important plant pathogens of a wide range of hosts worldwide. In the present study the species causing melanose and stem end rot diseases of Citrus spp. are revised. Three species of Diaporthe occurring on Citrus are characterised, including D. citri, D. cytosporella and D. foeniculina. Morphology and phylogenetic analyses of the complete nuclear ribosomal internal transcribed spacer regions and partial sequences of actin, beta-tubulin, calmodulin and translation elongation factor 1-α were used to resolve species on Citrus and related Diaporthe species. Diaporthe citri occurs on Citrus throughout the Citrus-growing regions of the world. Diaporthe cytosporella is found on Citrus in Europe and California (USA). Diaporthe foeniculina, including the synonym D. neotheicola, is recognised as a species with an extensive host range including Citrus. Diaporthe medusaea, a name widely used for D. citri, was determined to be a synonym of D. rudis, a species with a broad host range. Diaporthe citri is delimited based on molecular phylogenetic analysis with the inclusion of the conserved ex-type and additional collections from different geographic locations worldwide. Diaporthe cytosporella, D. foeniculina and D. rudis are epitypified, fully described and illustrated with a review of all synonyms based on molecular data and morphological studies. Newly designed primers are introduced to optimise the amplification and sequencing of calmodulin and actin genes in Diaporthe. A discussion is provided of the utility of genes and the need for multi-gene phylogenies when distinguishing species of Diaporthe or describing new species.

No MeSH data available.


Related in: MedlinePlus