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Novel genera and species of coniothyrium-like fungi in Montagnulaceae (Ascomycota).

Verkley GJ, Dukik K, Renfurm R, Göker M, Stielow JB - Persoonia (2014)

Bottom Line: In Paraphaeosphaeria five new species are described based on asexual morphs, viz.Paraph. arecacearum sp. nov., Paraph. neglecta sp. nov., Paraph. sardoa sp. nov., Paraph. verruculosa sp. nov., and Paraph. viridescens sp. nov.Macro- and micromorphological characteristics are fully described.

View Article: PubMed Central - PubMed

Affiliation: CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;

ABSTRACT
Based on analyses of concatenated internal transcribed spacer regions of the nrDNA operon (ITS), large subunit rDNA (LSU), γ-actin and β-tubulin gene sequences the taxonomy of coniothyrium-like fungi belonging in the family Montagnulaceae, order Pleosporales, was re-assessed. Two new genera are proposed, Alloconiothyrium, to accommodate A. aptrootii sp. nov., and Dendrothyrium for D. longisporum sp. nov. and D. variisporum sp. nov. One new species is described in Paraconiothyrium, viz. Parac. archidendri sp. nov., while two species so far classified in Paraconiothyrium are transferred to Paraphaeosphaeria, viz. Paraph. minitans comb. nov. and Paraph. sporulosa comb. nov. In Paraphaeosphaeria five new species are described based on asexual morphs, viz. Paraph. arecacearum sp. nov., Paraph. neglecta sp. nov., Paraph. sardoa sp. nov., Paraph. verruculosa sp. nov., and Paraph. viridescens sp. nov. Macro- and micromorphological characteristics are fully described.

No MeSH data available.


Midpoint-rooted maximum-likelihood phylogeny of Montagnulaceae inferred from four concatenated gene alignments (ITS, LSU, ACT and TUB) yielding a total of 2 286 characters. The numbers next to the branches are ML (left) and MP (right) bootstrap support values. The affiliations to species are highlighted. Species named in bold indicate taxa proposed in this study.
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Figure 2: Midpoint-rooted maximum-likelihood phylogeny of Montagnulaceae inferred from four concatenated gene alignments (ITS, LSU, ACT and TUB) yielding a total of 2 286 characters. The numbers next to the branches are ML (left) and MP (right) bootstrap support values. The affiliations to species are highlighted. Species named in bold indicate taxa proposed in this study.

Mentions: The performance of the four concatenated gene alignments (ITS, LSU, ACT and TUB) in combined and separate phylogenetic inference is shown in Table 2. The measures agreed that TUB provided overall the most support in combined and separate analysis, followed by ACT, ITS and LSU. Relative to the number of variable and parsimony-informative characters, however, ACT performed best, followed by LSU, TUB and ITS (in this respect, LSU performed even better than ACT when analysed separately). In the multi-locus phylogeny inferred from the combined dataset shown in Fig. 2, several well-supported clades can be identified, which are interpreted as appropriate for the delimitation of genera. The outgroup of the tree is formed by two highly supported clades representing the genera Alloconiothyrium (100/100 %) and Dendrothyrium (100/100 %). The Dendrothyrium clade comprises two species, with two isolates of D. longisporum (CBS 824.84, 582.83T) and the type strain of D. variisporum (CBS 121517T). A second strain, CBS 197.82, is also assigned to this species based on morphological similarities to the type strain, even though this renders the species paraphyletic in the presently postulated phylogeny, but without support. Another well-supported (100/98 %) clade forming the major part of the ingroup of the tree comprises 64 strains assigned to the genus Paraphaeosphaeria, with two isolates of Paraph. michotii, the type species of the genus, and the highly supported clades of the following species: Paraph. sporulosa (26 strains), Paraph. minitans (6), the new species Paraph. sardoa (1), Paraph. angularis (1), which clusters with Paraph. michotii and Paraph. pilleata, and furthermore Paraph. verruculosa (3), Paraph. neglecta (19), Paraph. arecacearum (2) and Paraph. viridescens (1). The intraspecific sequence variability regarding TUB is somewhat higher in Paraph. neglecta than in the other species of the genus with multiple strains in the tree, as indicated by partitioned Bremer support values for the interior branches of the Paraph. neglecta clade of 1–5 steps for TUB but ≤ 2 for the other genes (data not shown). CBS 101464 from Malawi deposited in CBS as Microsphaeropsis rugospora is found within the Paraphaeosphaeria clade (close to its base), and is preliminarily re-identified as Paraphaeosphaeria sp. The type of M. rugospora originated from cultivated soil in southern Japan (Someya et al. 1997).


Novel genera and species of coniothyrium-like fungi in Montagnulaceae (Ascomycota).

Verkley GJ, Dukik K, Renfurm R, Göker M, Stielow JB - Persoonia (2014)

Midpoint-rooted maximum-likelihood phylogeny of Montagnulaceae inferred from four concatenated gene alignments (ITS, LSU, ACT and TUB) yielding a total of 2 286 characters. The numbers next to the branches are ML (left) and MP (right) bootstrap support values. The affiliations to species are highlighted. Species named in bold indicate taxa proposed in this study.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150078&req=5

Figure 2: Midpoint-rooted maximum-likelihood phylogeny of Montagnulaceae inferred from four concatenated gene alignments (ITS, LSU, ACT and TUB) yielding a total of 2 286 characters. The numbers next to the branches are ML (left) and MP (right) bootstrap support values. The affiliations to species are highlighted. Species named in bold indicate taxa proposed in this study.
Mentions: The performance of the four concatenated gene alignments (ITS, LSU, ACT and TUB) in combined and separate phylogenetic inference is shown in Table 2. The measures agreed that TUB provided overall the most support in combined and separate analysis, followed by ACT, ITS and LSU. Relative to the number of variable and parsimony-informative characters, however, ACT performed best, followed by LSU, TUB and ITS (in this respect, LSU performed even better than ACT when analysed separately). In the multi-locus phylogeny inferred from the combined dataset shown in Fig. 2, several well-supported clades can be identified, which are interpreted as appropriate for the delimitation of genera. The outgroup of the tree is formed by two highly supported clades representing the genera Alloconiothyrium (100/100 %) and Dendrothyrium (100/100 %). The Dendrothyrium clade comprises two species, with two isolates of D. longisporum (CBS 824.84, 582.83T) and the type strain of D. variisporum (CBS 121517T). A second strain, CBS 197.82, is also assigned to this species based on morphological similarities to the type strain, even though this renders the species paraphyletic in the presently postulated phylogeny, but without support. Another well-supported (100/98 %) clade forming the major part of the ingroup of the tree comprises 64 strains assigned to the genus Paraphaeosphaeria, with two isolates of Paraph. michotii, the type species of the genus, and the highly supported clades of the following species: Paraph. sporulosa (26 strains), Paraph. minitans (6), the new species Paraph. sardoa (1), Paraph. angularis (1), which clusters with Paraph. michotii and Paraph. pilleata, and furthermore Paraph. verruculosa (3), Paraph. neglecta (19), Paraph. arecacearum (2) and Paraph. viridescens (1). The intraspecific sequence variability regarding TUB is somewhat higher in Paraph. neglecta than in the other species of the genus with multiple strains in the tree, as indicated by partitioned Bremer support values for the interior branches of the Paraph. neglecta clade of 1–5 steps for TUB but ≤ 2 for the other genes (data not shown). CBS 101464 from Malawi deposited in CBS as Microsphaeropsis rugospora is found within the Paraphaeosphaeria clade (close to its base), and is preliminarily re-identified as Paraphaeosphaeria sp. The type of M. rugospora originated from cultivated soil in southern Japan (Someya et al. 1997).

Bottom Line: In Paraphaeosphaeria five new species are described based on asexual morphs, viz.Paraph. arecacearum sp. nov., Paraph. neglecta sp. nov., Paraph. sardoa sp. nov., Paraph. verruculosa sp. nov., and Paraph. viridescens sp. nov.Macro- and micromorphological characteristics are fully described.

View Article: PubMed Central - PubMed

Affiliation: CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;

ABSTRACT
Based on analyses of concatenated internal transcribed spacer regions of the nrDNA operon (ITS), large subunit rDNA (LSU), γ-actin and β-tubulin gene sequences the taxonomy of coniothyrium-like fungi belonging in the family Montagnulaceae, order Pleosporales, was re-assessed. Two new genera are proposed, Alloconiothyrium, to accommodate A. aptrootii sp. nov., and Dendrothyrium for D. longisporum sp. nov. and D. variisporum sp. nov. One new species is described in Paraconiothyrium, viz. Parac. archidendri sp. nov., while two species so far classified in Paraconiothyrium are transferred to Paraphaeosphaeria, viz. Paraph. minitans comb. nov. and Paraph. sporulosa comb. nov. In Paraphaeosphaeria five new species are described based on asexual morphs, viz. Paraph. arecacearum sp. nov., Paraph. neglecta sp. nov., Paraph. sardoa sp. nov., Paraph. verruculosa sp. nov., and Paraph. viridescens sp. nov. Macro- and micromorphological characteristics are fully described.

No MeSH data available.