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Novel genera and species of coniothyrium-like fungi in Montagnulaceae (Ascomycota).

Verkley GJ, Dukik K, Renfurm R, Göker M, Stielow JB - Persoonia (2014)

Bottom Line: In Paraphaeosphaeria five new species are described based on asexual morphs, viz.Paraph. arecacearum sp. nov., Paraph. neglecta sp. nov., Paraph. sardoa sp. nov., Paraph. verruculosa sp. nov., and Paraph. viridescens sp. nov.Macro- and micromorphological characteristics are fully described.

View Article: PubMed Central - PubMed

Affiliation: CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;

ABSTRACT
Based on analyses of concatenated internal transcribed spacer regions of the nrDNA operon (ITS), large subunit rDNA (LSU), γ-actin and β-tubulin gene sequences the taxonomy of coniothyrium-like fungi belonging in the family Montagnulaceae, order Pleosporales, was re-assessed. Two new genera are proposed, Alloconiothyrium, to accommodate A. aptrootii sp. nov., and Dendrothyrium for D. longisporum sp. nov. and D. variisporum sp. nov. One new species is described in Paraconiothyrium, viz. Parac. archidendri sp. nov., while two species so far classified in Paraconiothyrium are transferred to Paraphaeosphaeria, viz. Paraph. minitans comb. nov. and Paraph. sporulosa comb. nov. In Paraphaeosphaeria five new species are described based on asexual morphs, viz. Paraph. arecacearum sp. nov., Paraph. neglecta sp. nov., Paraph. sardoa sp. nov., Paraph. verruculosa sp. nov., and Paraph. viridescens sp. nov. Macro- and micromorphological characteristics are fully described.

No MeSH data available.


Midpoint-rooted maximum-likelihood phylogeny of Coniothyrium-like Pleosporales and their relatives inferred from 890 LSU characters. The numbers abovenext to the branches are ML (left) and MP (right) bootstrap support values. Several large branches (marked by “//”) have been scaled to 25 % of original length to better fit the tree on page. Highlighted sections indicate affiliations to families.
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Figure 1: Midpoint-rooted maximum-likelihood phylogeny of Coniothyrium-like Pleosporales and their relatives inferred from 890 LSU characters. The numbers abovenext to the branches are ML (left) and MP (right) bootstrap support values. Several large branches (marked by “//”) have been scaled to 25 % of original length to better fit the tree on page. Highlighted sections indicate affiliations to families.

Mentions: The aligned LSU dataset used for determining the relationships between coniothyrium-like members of Pleosporales and their relatives comprised 172 organisms and 890 characters, including 290 variable and 248 parsimony-informative characters. The resulting ML tree is presented in Fig. 1 together with ML and MP bootstrap values. Strains representing the dark-spored coelomycete genera Asplosporella (Botryosphaeriales) and Phaeocytostroma (Diaporthales) form the outgroup and a small ingroup clade sister to all other ingroup clades, respectively. The pleosporalean taxa that constitute the major part of this tree group in clades that correspond to families that have previously been resolved in other molecular phylogenetic studies of Pleosporales (Schoch et al. 2009, Zhang et al. 2009, Aveskamp et al. 2010). One monophyletic group comprising 41 strains representing various families (bootstrap support 96/83 %) includes two strains of Coniothyrium palmarum, of the recently reinstated family Coniothyriaceae (de Gruyter et al. 2012), and Cucurbitaria berberidis (CBS 394.84) of the Cucurbitariaceae. Its subclade (89/63 %) representing the family Phaeosphaeriaceae comprises four subclades of its own, viz. a clade (98/79 %) of two strains identified as ‘Coniothyrium’ cerealis (CBS 518.74, 157.78), a second, well-supported (92/94 %) subclade of five strains of Ampelomyces quisqualis which reveals at least two distinct genotypes based on LSU. According to de Gruyter et al. (2009)Ampelomyces is heterogenous, with the type species A. quisqualis belonging in the Phaeosphaeriaceae, and A. quercinus in the Didymellaceae. Our data indicate that the three strains originating from cucumber mildew in Canada (CBS 128.79, 129.79, 131.79) are specifically distinct from USA strains CBS 131.31 and 133.32, from Erysiphe cichoracearum on Helianthus tuberosus and Microsphaera alni on Lonicera sp., respectively. A third, rather weakly supported (74/< 60 %) subclade with Phaeosphaeriopsis obtusispora (CBS 246.64), Phaeosphaeria occulta (CBS 582.86) and Parastagonospora nodorum (CBS 287.52, 272.59, 273.59), and a fourth, strongly supported subclade (100/98 %) with Phaeosph. avenaria (CBS 289.52, 385.86), Phaeosph. parvula (CBS 260.49, 605.86) and Phaeosph. eustoma (CBS 724.92, 307.71). The Didymellaceae clade (99/100 %) contains 10 strains, including Didymella exigua (CBS 183.51), the type species of the genus Didymella, Microsphaeropsis olivacea (CBS 233.77, 432.71) and two strains of ‘Coniothyrium’ nitidae (CBS 111302, 111321). Its unsupported sister group of miscellaneous fungi comprises Neophaeosphaeria filamentosa (CBS 102203), Coniothyrina agaves (CBS 470.69) (type species of the genus is C. agavicola), a well-supported subclade (100/100 %) with Leptosphaeria doliolum vars doliolum (CBS 297.51, 504.75) and errabunda (CBS 541.66), agreeing with Leptosphaeriaceae clade B of de Gruyter et al. (2012), and an incompletely resolved clade containing Plenodomus biglobosus (syn. Leptosphaeria biglobosa) (CBS 475.81, 476.81), Plenodomus lingam (syn. Leptosphaeria maculans, Phoma lingam) (CBS 147.24, 260.94) and Pleospora herbarum (CBS 191.86).


Novel genera and species of coniothyrium-like fungi in Montagnulaceae (Ascomycota).

Verkley GJ, Dukik K, Renfurm R, Göker M, Stielow JB - Persoonia (2014)

Midpoint-rooted maximum-likelihood phylogeny of Coniothyrium-like Pleosporales and their relatives inferred from 890 LSU characters. The numbers abovenext to the branches are ML (left) and MP (right) bootstrap support values. Several large branches (marked by “//”) have been scaled to 25 % of original length to better fit the tree on page. Highlighted sections indicate affiliations to families.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150078&req=5

Figure 1: Midpoint-rooted maximum-likelihood phylogeny of Coniothyrium-like Pleosporales and their relatives inferred from 890 LSU characters. The numbers abovenext to the branches are ML (left) and MP (right) bootstrap support values. Several large branches (marked by “//”) have been scaled to 25 % of original length to better fit the tree on page. Highlighted sections indicate affiliations to families.
Mentions: The aligned LSU dataset used for determining the relationships between coniothyrium-like members of Pleosporales and their relatives comprised 172 organisms and 890 characters, including 290 variable and 248 parsimony-informative characters. The resulting ML tree is presented in Fig. 1 together with ML and MP bootstrap values. Strains representing the dark-spored coelomycete genera Asplosporella (Botryosphaeriales) and Phaeocytostroma (Diaporthales) form the outgroup and a small ingroup clade sister to all other ingroup clades, respectively. The pleosporalean taxa that constitute the major part of this tree group in clades that correspond to families that have previously been resolved in other molecular phylogenetic studies of Pleosporales (Schoch et al. 2009, Zhang et al. 2009, Aveskamp et al. 2010). One monophyletic group comprising 41 strains representing various families (bootstrap support 96/83 %) includes two strains of Coniothyrium palmarum, of the recently reinstated family Coniothyriaceae (de Gruyter et al. 2012), and Cucurbitaria berberidis (CBS 394.84) of the Cucurbitariaceae. Its subclade (89/63 %) representing the family Phaeosphaeriaceae comprises four subclades of its own, viz. a clade (98/79 %) of two strains identified as ‘Coniothyrium’ cerealis (CBS 518.74, 157.78), a second, well-supported (92/94 %) subclade of five strains of Ampelomyces quisqualis which reveals at least two distinct genotypes based on LSU. According to de Gruyter et al. (2009)Ampelomyces is heterogenous, with the type species A. quisqualis belonging in the Phaeosphaeriaceae, and A. quercinus in the Didymellaceae. Our data indicate that the three strains originating from cucumber mildew in Canada (CBS 128.79, 129.79, 131.79) are specifically distinct from USA strains CBS 131.31 and 133.32, from Erysiphe cichoracearum on Helianthus tuberosus and Microsphaera alni on Lonicera sp., respectively. A third, rather weakly supported (74/< 60 %) subclade with Phaeosphaeriopsis obtusispora (CBS 246.64), Phaeosphaeria occulta (CBS 582.86) and Parastagonospora nodorum (CBS 287.52, 272.59, 273.59), and a fourth, strongly supported subclade (100/98 %) with Phaeosph. avenaria (CBS 289.52, 385.86), Phaeosph. parvula (CBS 260.49, 605.86) and Phaeosph. eustoma (CBS 724.92, 307.71). The Didymellaceae clade (99/100 %) contains 10 strains, including Didymella exigua (CBS 183.51), the type species of the genus Didymella, Microsphaeropsis olivacea (CBS 233.77, 432.71) and two strains of ‘Coniothyrium’ nitidae (CBS 111302, 111321). Its unsupported sister group of miscellaneous fungi comprises Neophaeosphaeria filamentosa (CBS 102203), Coniothyrina agaves (CBS 470.69) (type species of the genus is C. agavicola), a well-supported subclade (100/100 %) with Leptosphaeria doliolum vars doliolum (CBS 297.51, 504.75) and errabunda (CBS 541.66), agreeing with Leptosphaeriaceae clade B of de Gruyter et al. (2012), and an incompletely resolved clade containing Plenodomus biglobosus (syn. Leptosphaeria biglobosa) (CBS 475.81, 476.81), Plenodomus lingam (syn. Leptosphaeria maculans, Phoma lingam) (CBS 147.24, 260.94) and Pleospora herbarum (CBS 191.86).

Bottom Line: In Paraphaeosphaeria five new species are described based on asexual morphs, viz.Paraph. arecacearum sp. nov., Paraph. neglecta sp. nov., Paraph. sardoa sp. nov., Paraph. verruculosa sp. nov., and Paraph. viridescens sp. nov.Macro- and micromorphological characteristics are fully described.

View Article: PubMed Central - PubMed

Affiliation: CBS-KNAW Fungal Biodiversity Centre, P.O. Box 85167, 3508 AD Utrecht, The Netherlands;

ABSTRACT
Based on analyses of concatenated internal transcribed spacer regions of the nrDNA operon (ITS), large subunit rDNA (LSU), γ-actin and β-tubulin gene sequences the taxonomy of coniothyrium-like fungi belonging in the family Montagnulaceae, order Pleosporales, was re-assessed. Two new genera are proposed, Alloconiothyrium, to accommodate A. aptrootii sp. nov., and Dendrothyrium for D. longisporum sp. nov. and D. variisporum sp. nov. One new species is described in Paraconiothyrium, viz. Parac. archidendri sp. nov., while two species so far classified in Paraconiothyrium are transferred to Paraphaeosphaeria, viz. Paraph. minitans comb. nov. and Paraph. sporulosa comb. nov. In Paraphaeosphaeria five new species are described based on asexual morphs, viz. Paraph. arecacearum sp. nov., Paraph. neglecta sp. nov., Paraph. sardoa sp. nov., Paraph. verruculosa sp. nov., and Paraph. viridescens sp. nov. Macro- and micromorphological characteristics are fully described.

No MeSH data available.