Limits...
Phylogeny and taxonomy of the genus Gliocephalotrichum.

Lombard L, Serrato-Diaz LM, Cheewangkoon R, French-Monar RD, Decock C, Crous PW - Persoonia (2014)

Bottom Line: Contemporary taxonomic studies of these fungi have relied on morphology and DNA sequence comparisons of the internal transcribed spacer region of the nuclear rDNA (ITS) and the β-tubulin gene regions.As a result five species are newly described, namely G. humicola (Taiwan, soil), G. mexicanum (rambutan fruit from Mexico), G. nephelii (rambutan fruit from Guatemala), G. queenslandicum (Australia, endophytic isolations) and G. simmonsii (rambutan fruit from Guatemala).Although species of Gliocephalotrichum are generally not regarded as important plant pathogens, their ability to cause post-harvest fruit rot could have an impact on fruit export and storage.

View Article: PubMed Central - PubMed

Affiliation: CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands;

ABSTRACT
Species in the genus Gliocephalotrichum (= Leuconectria) (Hypocreales, Nectriaceae) are soilborne fungi, associated with post-harvest fruit spoilage of several important tropical fruit crops. Contemporary taxonomic studies of these fungi have relied on morphology and DNA sequence comparisons of the internal transcribed spacer region of the nuclear rDNA (ITS) and the β-tubulin gene regions. Employing DNA sequence data from four loci (β-tubulin, histone H3, ITS, and translation elongation factor 1-alpha) and morphological comparisons, the taxonomic status of the genus Gliocephalotrichum was re-evaluated. As a result five species are newly described, namely G. humicola (Taiwan, soil), G. mexicanum (rambutan fruit from Mexico), G. nephelii (rambutan fruit from Guatemala), G. queenslandicum (Australia, endophytic isolations) and G. simmonsii (rambutan fruit from Guatemala). Although species of Gliocephalotrichum are generally not regarded as important plant pathogens, their ability to cause post-harvest fruit rot could have an impact on fruit export and storage.

No MeSH data available.


One of 1 000 most parsimonious trees obtained from a heuristic search with 1 000 random addition sequences of the combined sequences of β-tubulin, histone H3, internal transcribed spacer region and translation elongation factor 1-alpha sequence alignments of the Gliocephalotrichum isolates used in this study. Scale bar shows 10 changes. Bootstrap support values and Bayesian posterior probability values are shown at the nodes. Thickened lines indicate branches in the strict consensus tree and the consensus tree of the Bayesian analysis. The tree was rooted to Gliocladiopsis tenuis (IMI 68205) and Gliocladiopsis sagariensis (CBS 199.55). Ex-type isolates are indicated in bold.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4150073&req=5

Figure 1: One of 1 000 most parsimonious trees obtained from a heuristic search with 1 000 random addition sequences of the combined sequences of β-tubulin, histone H3, internal transcribed spacer region and translation elongation factor 1-alpha sequence alignments of the Gliocephalotrichum isolates used in this study. Scale bar shows 10 changes. Bootstrap support values and Bayesian posterior probability values are shown at the nodes. Thickened lines indicate branches in the strict consensus tree and the consensus tree of the Bayesian analysis. The tree was rooted to Gliocladiopsis tenuis (IMI 68205) and Gliocladiopsis sagariensis (CBS 199.55). Ex-type isolates are indicated in bold.

Mentions: Amplicons of around 500–550 bp were determined for the four genes used in this study. The phylogenetic analyses included 70 ingroup taxa, with Gliocladiopsis sagariensis (CBS 199.55) and G. tenuis (IMI 68205) as outgroup taxa (Lombard & Crous 2012). No topological conflicts were found between the four partitions based on the reciprocal 70 % BS threshold and therefore the sequence datasets were combined. The combined sequence dataset consisted of 2 491 characters, including alignment gaps. Of these, 1 427 were constant, 159 parsimony-uninformative and 905 parsimony-informative. The MP analysis yielded 1 000 trees (TL = 2716; CI = 0.640; RI = 0.921; RC = 0.590), of which the first is presented (Fig. 1). For the Bayesian inference, a HKY+I+G model was selected for BTUB and TEF, GTR+I+G for HIS3, and SYM+I+G for ITS which was incorporated into the analyses. The Bayesian consensus tree confirmed the tree topology and bootstrap support of the strict consensus tree obtained with MP.


Phylogeny and taxonomy of the genus Gliocephalotrichum.

Lombard L, Serrato-Diaz LM, Cheewangkoon R, French-Monar RD, Decock C, Crous PW - Persoonia (2014)

One of 1 000 most parsimonious trees obtained from a heuristic search with 1 000 random addition sequences of the combined sequences of β-tubulin, histone H3, internal transcribed spacer region and translation elongation factor 1-alpha sequence alignments of the Gliocephalotrichum isolates used in this study. Scale bar shows 10 changes. Bootstrap support values and Bayesian posterior probability values are shown at the nodes. Thickened lines indicate branches in the strict consensus tree and the consensus tree of the Bayesian analysis. The tree was rooted to Gliocladiopsis tenuis (IMI 68205) and Gliocladiopsis sagariensis (CBS 199.55). Ex-type isolates are indicated in bold.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150073&req=5

Figure 1: One of 1 000 most parsimonious trees obtained from a heuristic search with 1 000 random addition sequences of the combined sequences of β-tubulin, histone H3, internal transcribed spacer region and translation elongation factor 1-alpha sequence alignments of the Gliocephalotrichum isolates used in this study. Scale bar shows 10 changes. Bootstrap support values and Bayesian posterior probability values are shown at the nodes. Thickened lines indicate branches in the strict consensus tree and the consensus tree of the Bayesian analysis. The tree was rooted to Gliocladiopsis tenuis (IMI 68205) and Gliocladiopsis sagariensis (CBS 199.55). Ex-type isolates are indicated in bold.
Mentions: Amplicons of around 500–550 bp were determined for the four genes used in this study. The phylogenetic analyses included 70 ingroup taxa, with Gliocladiopsis sagariensis (CBS 199.55) and G. tenuis (IMI 68205) as outgroup taxa (Lombard & Crous 2012). No topological conflicts were found between the four partitions based on the reciprocal 70 % BS threshold and therefore the sequence datasets were combined. The combined sequence dataset consisted of 2 491 characters, including alignment gaps. Of these, 1 427 were constant, 159 parsimony-uninformative and 905 parsimony-informative. The MP analysis yielded 1 000 trees (TL = 2716; CI = 0.640; RI = 0.921; RC = 0.590), of which the first is presented (Fig. 1). For the Bayesian inference, a HKY+I+G model was selected for BTUB and TEF, GTR+I+G for HIS3, and SYM+I+G for ITS which was incorporated into the analyses. The Bayesian consensus tree confirmed the tree topology and bootstrap support of the strict consensus tree obtained with MP.

Bottom Line: Contemporary taxonomic studies of these fungi have relied on morphology and DNA sequence comparisons of the internal transcribed spacer region of the nuclear rDNA (ITS) and the β-tubulin gene regions.As a result five species are newly described, namely G. humicola (Taiwan, soil), G. mexicanum (rambutan fruit from Mexico), G. nephelii (rambutan fruit from Guatemala), G. queenslandicum (Australia, endophytic isolations) and G. simmonsii (rambutan fruit from Guatemala).Although species of Gliocephalotrichum are generally not regarded as important plant pathogens, their ability to cause post-harvest fruit rot could have an impact on fruit export and storage.

View Article: PubMed Central - PubMed

Affiliation: CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands;

ABSTRACT
Species in the genus Gliocephalotrichum (= Leuconectria) (Hypocreales, Nectriaceae) are soilborne fungi, associated with post-harvest fruit spoilage of several important tropical fruit crops. Contemporary taxonomic studies of these fungi have relied on morphology and DNA sequence comparisons of the internal transcribed spacer region of the nuclear rDNA (ITS) and the β-tubulin gene regions. Employing DNA sequence data from four loci (β-tubulin, histone H3, ITS, and translation elongation factor 1-alpha) and morphological comparisons, the taxonomic status of the genus Gliocephalotrichum was re-evaluated. As a result five species are newly described, namely G. humicola (Taiwan, soil), G. mexicanum (rambutan fruit from Mexico), G. nephelii (rambutan fruit from Guatemala), G. queenslandicum (Australia, endophytic isolations) and G. simmonsii (rambutan fruit from Guatemala). Although species of Gliocephalotrichum are generally not regarded as important plant pathogens, their ability to cause post-harvest fruit rot could have an impact on fruit export and storage.

No MeSH data available.