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Phylogenetic circumscription of Arthrographis (Eremomycetaceae, Dothideomycetes).

Giraldo A, Gené J, Sutton DA, Madrid H, Cano J, Crous PW, Guarro J - Persoonia (2014)

Bottom Line: Based on these results, four new species are recognised, namely Arthrographis chlamydospora, A. curvata, A. globosa and A. longispora.Arthrographis longispora also produces membranous colonies, but has poorly differentiated conidiophores and long arthroconidia.Morphological variants are described for A. kalrae and our results also revealed that Eremomyces langeronii and A. kalrae, traditionally considered the sexual and asexual morphs of the same species, are not conspecific.

View Article: PubMed Central - PubMed

Affiliation: Unitat de Micologia, Facultat de Medicina i Ciències de la Salut and IISPV, Universitat Rovira i Virgili, Sant Llorenç 21, 43201 Reus, Tarragona, Spain;

ABSTRACT
Numerous members of Ascomycota and Basidiomycota produce only poorly differentiated arthroconidial asexual morphs in culture. These arthroconidial fungi are grouped in genera where the asexual-sexual connections and their taxonomic circumscription are poorly known. In the present study we explored the phylogenetic relationships of two of these ascomycetous genera, Arthrographis and Arthropsis. Analysis of D1/D2 sequences of all species of both genera revealed that both are polyphyletic, with species being accommodated in different orders and classes. Because genetic variability was detected among reference strains and fresh isolates resembling the genus Arthrographis, we carried out a detailed phenotypic and phylogenetic analysis based on sequence data of the ITS region, actin and chitin synthase genes. Based on these results, four new species are recognised, namely Arthrographis chlamydospora, A. curvata, A. globosa and A. longispora. Arthrographis chlamydospora is distinguished by its cerebriform colonies, branched conidiophores, cuboid arthroconidia and terminal or intercalary globose to subglobose chlamydospores. Arthrographis curvata produced both sexual and asexual morphs, and is characterised by navicular ascospores and dimorphic conidia, namely cylindrical arthroconidia and curved, cashewnut-shaped conidia formed laterally on vegetative hyphae. Arthrographis globosa produced membranous colonies, but is mainly characterised by doliiform to globose arthroconidia. Arthrographis longispora also produces membranous colonies, but has poorly differentiated conidiophores and long arthroconidia. Morphological variants are described for A. kalrae and our results also revealed that Eremomyces langeronii and A. kalrae, traditionally considered the sexual and asexual morphs of the same species, are not conspecific.

No MeSH data available.


Related in: MedlinePlus

Maximum-likelihood (ML) tree constructed with sequences of the D1/D2 domains of the 28S rRNA gene. Bootstrap support values above 70 % are indicated at the nodes. The phylogenetic tree was rooted to Peziza varia and Peziza phyllogena. T = Ex-type strain.
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Figure 1: Maximum-likelihood (ML) tree constructed with sequences of the D1/D2 domains of the 28S rRNA gene. Bootstrap support values above 70 % are indicated at the nodes. The phylogenetic tree was rooted to Peziza varia and Peziza phyllogena. T = Ex-type strain.

Mentions: The D1/D2 phylogenetic tree that included the ex-type strains of the different species of Arthrographis and Arthropsis and representative members of different fungal classes and orders revealed that both genera are polyphyletic (Fig. 1). The type species of Arthrographis, A. kalrae, was included in a well-supported clade within the Dothideomycetes (85 % bootstrap, bs), forming together a highly supported subclade (99 % bs) with Rhexothecium globosum, Eremomyces bilateralis, E. langeronii and four unidentified species of Arthrographis. While the ex-type strain of Arthrographis lignicola was related to different genera of the Lecanoromycetes, such as Sarea and Pycnora, the ex-type strains of Arthrographis pinicola and A. alba were associated with the Eurotiomycetes (99 % bs). Arthrographis pinicola and Eremascus albus (Eremascaceae) formed a well-supported clade (93 % bs), while A. alba and Leucothecium emdenii formed a well-supported clade (99 % bs) within the Onygenales.


Phylogenetic circumscription of Arthrographis (Eremomycetaceae, Dothideomycetes).

Giraldo A, Gené J, Sutton DA, Madrid H, Cano J, Crous PW, Guarro J - Persoonia (2014)

Maximum-likelihood (ML) tree constructed with sequences of the D1/D2 domains of the 28S rRNA gene. Bootstrap support values above 70 % are indicated at the nodes. The phylogenetic tree was rooted to Peziza varia and Peziza phyllogena. T = Ex-type strain.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150071&req=5

Figure 1: Maximum-likelihood (ML) tree constructed with sequences of the D1/D2 domains of the 28S rRNA gene. Bootstrap support values above 70 % are indicated at the nodes. The phylogenetic tree was rooted to Peziza varia and Peziza phyllogena. T = Ex-type strain.
Mentions: The D1/D2 phylogenetic tree that included the ex-type strains of the different species of Arthrographis and Arthropsis and representative members of different fungal classes and orders revealed that both genera are polyphyletic (Fig. 1). The type species of Arthrographis, A. kalrae, was included in a well-supported clade within the Dothideomycetes (85 % bootstrap, bs), forming together a highly supported subclade (99 % bs) with Rhexothecium globosum, Eremomyces bilateralis, E. langeronii and four unidentified species of Arthrographis. While the ex-type strain of Arthrographis lignicola was related to different genera of the Lecanoromycetes, such as Sarea and Pycnora, the ex-type strains of Arthrographis pinicola and A. alba were associated with the Eurotiomycetes (99 % bs). Arthrographis pinicola and Eremascus albus (Eremascaceae) formed a well-supported clade (93 % bs), while A. alba and Leucothecium emdenii formed a well-supported clade (99 % bs) within the Onygenales.

Bottom Line: Based on these results, four new species are recognised, namely Arthrographis chlamydospora, A. curvata, A. globosa and A. longispora.Arthrographis longispora also produces membranous colonies, but has poorly differentiated conidiophores and long arthroconidia.Morphological variants are described for A. kalrae and our results also revealed that Eremomyces langeronii and A. kalrae, traditionally considered the sexual and asexual morphs of the same species, are not conspecific.

View Article: PubMed Central - PubMed

Affiliation: Unitat de Micologia, Facultat de Medicina i Ciències de la Salut and IISPV, Universitat Rovira i Virgili, Sant Llorenç 21, 43201 Reus, Tarragona, Spain;

ABSTRACT
Numerous members of Ascomycota and Basidiomycota produce only poorly differentiated arthroconidial asexual morphs in culture. These arthroconidial fungi are grouped in genera where the asexual-sexual connections and their taxonomic circumscription are poorly known. In the present study we explored the phylogenetic relationships of two of these ascomycetous genera, Arthrographis and Arthropsis. Analysis of D1/D2 sequences of all species of both genera revealed that both are polyphyletic, with species being accommodated in different orders and classes. Because genetic variability was detected among reference strains and fresh isolates resembling the genus Arthrographis, we carried out a detailed phenotypic and phylogenetic analysis based on sequence data of the ITS region, actin and chitin synthase genes. Based on these results, four new species are recognised, namely Arthrographis chlamydospora, A. curvata, A. globosa and A. longispora. Arthrographis chlamydospora is distinguished by its cerebriform colonies, branched conidiophores, cuboid arthroconidia and terminal or intercalary globose to subglobose chlamydospores. Arthrographis curvata produced both sexual and asexual morphs, and is characterised by navicular ascospores and dimorphic conidia, namely cylindrical arthroconidia and curved, cashewnut-shaped conidia formed laterally on vegetative hyphae. Arthrographis globosa produced membranous colonies, but is mainly characterised by doliiform to globose arthroconidia. Arthrographis longispora also produces membranous colonies, but has poorly differentiated conidiophores and long arthroconidia. Morphological variants are described for A. kalrae and our results also revealed that Eremomyces langeronii and A. kalrae, traditionally considered the sexual and asexual morphs of the same species, are not conspecific.

No MeSH data available.


Related in: MedlinePlus