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A phylogenetic study of Dothiorella and Spencermartinsia species associated with woody plants in Iran, New Zealand, Portugal and Spain.

Abdollahzadeh J, Javadi A, Zare R, Phillips AJ - Persoonia (2014)

Bottom Line: Dothiorella and Spencermartinsia are two botryosphaeriaceous genera with dark 2-celled conidia and found in parasitic, saprophytic or endophytic association with various woody host plants.Of these, five species are placed in Dothiorella, namely D. iranica, D. parva, D. prunicola, D. sempervirentis and D. striata, and three species belong to Spencermartinsia named as S. citricola, S. mangiferae and S. plurivora.An identification key to the species of each genus is provided.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Protection, Faculty of Agriculture, University of Kurdistan, P.O. Box 416, Sanandaj, Iran;

ABSTRACT
Dothiorella and Spencermartinsia are two botryosphaeriaceous genera with dark 2-celled conidia and found in parasitic, saprophytic or endophytic association with various woody host plants. Based on ITS and EF1-α sequence data and morphology, eight new species are described from Iran, New Zealand, Portugal and Spain. Of these, five species are placed in Dothiorella, namely D. iranica, D. parva, D. prunicola, D. sempervirentis and D. striata, and three species belong to Spencermartinsia named as S. citricola, S. mangiferae and S. plurivora. An identification key to the species of each genus is provided.

No MeSH data available.


Related in: MedlinePlus

Distance tree obtained using the K2P substitution model on the combined ITS and EF1-α sequence data. BI/MP/NJ posterior probabilities and bootstrap support values are given at the nodes. The tree is rooted to Neofusicoccum luteum (CBS 110299, CBS 110497). New species and ex-type strains are in bold face.
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Figure 1: Distance tree obtained using the K2P substitution model on the combined ITS and EF1-α sequence data. BI/MP/NJ posterior probabilities and bootstrap support values are given at the nodes. The tree is rooted to Neofusicoccum luteum (CBS 110299, CBS 110497). New species and ex-type strains are in bold face.

Mentions: Phylogenetic analyses were done based on different combination of the three unlinked gene regions including ITS/EF1-α, ITS/β-tubulin and ITS/EF1-α/β-tubulin. The phylogenies resulted from ITS/EF1-α were stable and reproducible with highly supported internal nodes, while β-tubulin did not improve the phylogenies so that combination of ITS and β-tubulin datasets resulted in poor and distinct phylogenies in different analyses (data not shown). Furthermore, PHT test for combined ITS/EF1-α/β-tubulin datasets was significant (P < 0.01). Therefore, β-tubulin was excluded and phylogenetic analyses were based on ITS and EF1-α sequences. The partition homogeneity test (P = 0.11) indicated the phylogenies resulting from ITS and EF1-α were congruent so the ITS and EF1-α datasets were combined in a single analysis. The combined ITS and EF1-α sequences for 57 ingroup and 2 outgroup taxa contained 1 108 characters including alignment gaps, of which 334 characters were excluded, 528 were constant, 23 were variable and parsimony-uninformative and 223 were parsimony-informative. A heuristic search of the remaining 223 parsimony-informative characters resulted in a single most parsimonious tree of 426 steps (CI = 0.7, HI = 0.3, RI = 0.93). The Bayesian and NJ analyses produced phylogenetic trees with the same topology as the MP tree. The NJ tree is shown in Fig. 1 with BI/MP/NJ posterior probabilities and bootstrap support values at the nodes. MP and Bayesian trees are available in TreeBASE (S14150). In these phylogenetic analyses 22 subclades, representing 22 species of Botryosphaeriaceae with dark-walled 2-celled conidia, were recognized in two major clades corresponding to Dothiorella with 18 subclades, and Spencermartinsia with four subclades. Of these, nine subclades are recognised here as representatives of nine new species for the science.


A phylogenetic study of Dothiorella and Spencermartinsia species associated with woody plants in Iran, New Zealand, Portugal and Spain.

Abdollahzadeh J, Javadi A, Zare R, Phillips AJ - Persoonia (2014)

Distance tree obtained using the K2P substitution model on the combined ITS and EF1-α sequence data. BI/MP/NJ posterior probabilities and bootstrap support values are given at the nodes. The tree is rooted to Neofusicoccum luteum (CBS 110299, CBS 110497). New species and ex-type strains are in bold face.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150070&req=5

Figure 1: Distance tree obtained using the K2P substitution model on the combined ITS and EF1-α sequence data. BI/MP/NJ posterior probabilities and bootstrap support values are given at the nodes. The tree is rooted to Neofusicoccum luteum (CBS 110299, CBS 110497). New species and ex-type strains are in bold face.
Mentions: Phylogenetic analyses were done based on different combination of the three unlinked gene regions including ITS/EF1-α, ITS/β-tubulin and ITS/EF1-α/β-tubulin. The phylogenies resulted from ITS/EF1-α were stable and reproducible with highly supported internal nodes, while β-tubulin did not improve the phylogenies so that combination of ITS and β-tubulin datasets resulted in poor and distinct phylogenies in different analyses (data not shown). Furthermore, PHT test for combined ITS/EF1-α/β-tubulin datasets was significant (P < 0.01). Therefore, β-tubulin was excluded and phylogenetic analyses were based on ITS and EF1-α sequences. The partition homogeneity test (P = 0.11) indicated the phylogenies resulting from ITS and EF1-α were congruent so the ITS and EF1-α datasets were combined in a single analysis. The combined ITS and EF1-α sequences for 57 ingroup and 2 outgroup taxa contained 1 108 characters including alignment gaps, of which 334 characters were excluded, 528 were constant, 23 were variable and parsimony-uninformative and 223 were parsimony-informative. A heuristic search of the remaining 223 parsimony-informative characters resulted in a single most parsimonious tree of 426 steps (CI = 0.7, HI = 0.3, RI = 0.93). The Bayesian and NJ analyses produced phylogenetic trees with the same topology as the MP tree. The NJ tree is shown in Fig. 1 with BI/MP/NJ posterior probabilities and bootstrap support values at the nodes. MP and Bayesian trees are available in TreeBASE (S14150). In these phylogenetic analyses 22 subclades, representing 22 species of Botryosphaeriaceae with dark-walled 2-celled conidia, were recognized in two major clades corresponding to Dothiorella with 18 subclades, and Spencermartinsia with four subclades. Of these, nine subclades are recognised here as representatives of nine new species for the science.

Bottom Line: Dothiorella and Spencermartinsia are two botryosphaeriaceous genera with dark 2-celled conidia and found in parasitic, saprophytic or endophytic association with various woody host plants.Of these, five species are placed in Dothiorella, namely D. iranica, D. parva, D. prunicola, D. sempervirentis and D. striata, and three species belong to Spencermartinsia named as S. citricola, S. mangiferae and S. plurivora.An identification key to the species of each genus is provided.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Protection, Faculty of Agriculture, University of Kurdistan, P.O. Box 416, Sanandaj, Iran;

ABSTRACT
Dothiorella and Spencermartinsia are two botryosphaeriaceous genera with dark 2-celled conidia and found in parasitic, saprophytic or endophytic association with various woody host plants. Based on ITS and EF1-α sequence data and morphology, eight new species are described from Iran, New Zealand, Portugal and Spain. Of these, five species are placed in Dothiorella, namely D. iranica, D. parva, D. prunicola, D. sempervirentis and D. striata, and three species belong to Spencermartinsia named as S. citricola, S. mangiferae and S. plurivora. An identification key to the species of each genus is provided.

No MeSH data available.


Related in: MedlinePlus