p300-mediated acetylation of COMMD1 regulates its stability, and the ubiquitylation and nucleolar translocation of the RelA NF-κB subunit.
Bottom Line: We show that p300 is required for stress-mediated ubiquitylation and nucleolar translocation of RelA, but that this effect is indirect.In contrast, tumour necrosis factor (TNF) has no effect on COMMD1 acetylation.Finally, we demonstrate these findings have relevance in a whole tissue setting.
Affiliation: Edinburgh Cancer Research Centre, IGMM, University of Edinburgh, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK.Show MeSH
Related in: MedlinePlus
Mentions: Previous studies have indicated COMMD1 is ubiquitylated by the E3 ligase XIAP, then targeted for degradation (Mufti et al., 2007). Therefore, we next determined whether this pathway was involved in COMMD1 regulation by p300. Fig. 3A demonstrates that the reduction in COMMD1 observed upon loss of p300 is reversed by concomitant depletion of XIAP.
Affiliation: Edinburgh Cancer Research Centre, IGMM, University of Edinburgh, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK.