Conditional independence mapping of DIGE data reveals PDIA3 protein species as key nodes associated with muscle aerobic capacity.
Bottom Line: Forty protein species were differentially (P<0.05, FDR<10%) expressed between HCR and LCR and conditional independence mapping found distinct networks within these data, which brought insight beyond that achieved by functional annotation.Instead we found that noncanonical STAT3 signalling may be associated with low exercise capacity and skeletal muscle insulin resistance.Moreover, we demonstrate that this novel approach can be applied to 2D gel analysis, which is unsurpassed in its ability to profile protein species but currently has few dedicated bioinformatic tools.
Affiliation: Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK. Electronic address: email@example.com.Show MeSH
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Mentions: The abundance of STAT3 protein was not significantly different between male and female or HCR and LCR soleus muscles (Fig. 5). Similarly, there was no significant difference in the relative ratio of tyrosine (Y705) phosphorylation of STAT3. However, serine (S727) phosphorylation of STAT3 was 1.54-fold greater (P = 0.0058) in LCR soleus.
Affiliation: Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK. Electronic address: firstname.lastname@example.org.