Limits...
Conditional independence mapping of DIGE data reveals PDIA3 protein species as key nodes associated with muscle aerobic capacity.

Burniston JG, Kenyani J, Gray D, Guadagnin E, Jarman IH, Cobley JN, Cuthbertson DJ, Chen YW, Wastling JM, Lisboa PJ, Koch LG, Britton SL - J Proteomics (2014)

Bottom Line: Forty protein species were differentially (P<0.05, FDR<10%) expressed between HCR and LCR and conditional independence mapping found distinct networks within these data, which brought insight beyond that achieved by functional annotation.Instead we found that noncanonical STAT3 signalling may be associated with low exercise capacity and skeletal muscle insulin resistance.Moreover, we demonstrate that this novel approach can be applied to 2D gel analysis, which is unsurpassed in its ability to profile protein species but currently has few dedicated bioinformatic tools.

View Article: PubMed Central - PubMed

Affiliation: Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK. Electronic address: j.burniston@ljmu.ac.uk.

Show MeSH

Related in: MedlinePlus

STAT3 phosphorylation in male and female HCR/LCR muscle. STAT3 protein abundance and relative phosphorylation of either tyrosine (Y705) or serine (S727) residues was measured in the solei of male and female, HCR and LCR animals (A). Two-way analysis of variance found no significant difference in the abundance of STAT3 (B) or in the relative amount of Y705 phosphorylation (C). In contrast, a significant (*P < 0.05) main effect of ‘strain’ (i.e. HCR vs LCR) was evident for S727 phosphorylation. On average, the extent of S727 phosphorylation was 1.54-fold greater in LCR soleus (D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4150023&req=5

Figure 5: STAT3 phosphorylation in male and female HCR/LCR muscle. STAT3 protein abundance and relative phosphorylation of either tyrosine (Y705) or serine (S727) residues was measured in the solei of male and female, HCR and LCR animals (A). Two-way analysis of variance found no significant difference in the abundance of STAT3 (B) or in the relative amount of Y705 phosphorylation (C). In contrast, a significant (*P < 0.05) main effect of ‘strain’ (i.e. HCR vs LCR) was evident for S727 phosphorylation. On average, the extent of S727 phosphorylation was 1.54-fold greater in LCR soleus (D).

Mentions: The abundance of STAT3 protein was not significantly different between male and female or HCR and LCR soleus muscles (Fig. 5). Similarly, there was no significant difference in the relative ratio of tyrosine (Y705) phosphorylation of STAT3. However, serine (S727) phosphorylation of STAT3 was 1.54-fold greater (P = 0.0058) in LCR soleus.


Conditional independence mapping of DIGE data reveals PDIA3 protein species as key nodes associated with muscle aerobic capacity.

Burniston JG, Kenyani J, Gray D, Guadagnin E, Jarman IH, Cobley JN, Cuthbertson DJ, Chen YW, Wastling JM, Lisboa PJ, Koch LG, Britton SL - J Proteomics (2014)

STAT3 phosphorylation in male and female HCR/LCR muscle. STAT3 protein abundance and relative phosphorylation of either tyrosine (Y705) or serine (S727) residues was measured in the solei of male and female, HCR and LCR animals (A). Two-way analysis of variance found no significant difference in the abundance of STAT3 (B) or in the relative amount of Y705 phosphorylation (C). In contrast, a significant (*P < 0.05) main effect of ‘strain’ (i.e. HCR vs LCR) was evident for S727 phosphorylation. On average, the extent of S727 phosphorylation was 1.54-fold greater in LCR soleus (D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150023&req=5

Figure 5: STAT3 phosphorylation in male and female HCR/LCR muscle. STAT3 protein abundance and relative phosphorylation of either tyrosine (Y705) or serine (S727) residues was measured in the solei of male and female, HCR and LCR animals (A). Two-way analysis of variance found no significant difference in the abundance of STAT3 (B) or in the relative amount of Y705 phosphorylation (C). In contrast, a significant (*P < 0.05) main effect of ‘strain’ (i.e. HCR vs LCR) was evident for S727 phosphorylation. On average, the extent of S727 phosphorylation was 1.54-fold greater in LCR soleus (D).
Mentions: The abundance of STAT3 protein was not significantly different between male and female or HCR and LCR soleus muscles (Fig. 5). Similarly, there was no significant difference in the relative ratio of tyrosine (Y705) phosphorylation of STAT3. However, serine (S727) phosphorylation of STAT3 was 1.54-fold greater (P = 0.0058) in LCR soleus.

Bottom Line: Forty protein species were differentially (P<0.05, FDR<10%) expressed between HCR and LCR and conditional independence mapping found distinct networks within these data, which brought insight beyond that achieved by functional annotation.Instead we found that noncanonical STAT3 signalling may be associated with low exercise capacity and skeletal muscle insulin resistance.Moreover, we demonstrate that this novel approach can be applied to 2D gel analysis, which is unsurpassed in its ability to profile protein species but currently has few dedicated bioinformatic tools.

View Article: PubMed Central - PubMed

Affiliation: Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK. Electronic address: j.burniston@ljmu.ac.uk.

Show MeSH
Related in: MedlinePlus