Conditional independence mapping of DIGE data reveals PDIA3 protein species as key nodes associated with muscle aerobic capacity.
Bottom Line: Forty protein species were differentially (P<0.05, FDR<10%) expressed between HCR and LCR and conditional independence mapping found distinct networks within these data, which brought insight beyond that achieved by functional annotation.Instead we found that noncanonical STAT3 signalling may be associated with low exercise capacity and skeletal muscle insulin resistance.Moreover, we demonstrate that this novel approach can be applied to 2D gel analysis, which is unsurpassed in its ability to profile protein species but currently has few dedicated bioinformatic tools.
Affiliation: Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK. Electronic address: firstname.lastname@example.org.Show MeSH
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Mentions: A bibliometric network was constructed as an unbiased means to explore literature evidence of proteins that have been associated with PDIA3 (Fig. 4). The majority of citations were related to protein–protein interactions between PDIA3 and the ER chaperones calnexin and calreticulin. Second to these interactions was strong evidence of the association of PDIA3 with the signal transducer and activator of transcription 3 (STAT3), which encompassed evidence from co-expression, co-localisation, protein–protein interaction and gain/loss of function studies.
Affiliation: Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK. Electronic address: email@example.com.