Limits...
Moxibustion Activates Macrophage Autophagy and Protects Experimental Mice against Bacterial Infection.

Li X, Guo G, Shen F, Kong L, Liang F, Sun G - Evid Based Complement Alternat Med (2014)

Bottom Line: Moxibustion is one of main therapies in traditional Chinese medicine and uses heat stimulation on the body surface from the burning of moxa to release pain or treat diseases.Here we show moxibustion promoted profound macrophage autophagy in experimental Kunming mice, with reduced Akt phosphorylation and activated eIF2α phosphorylation.Consequently, moxibustion promoted bacterial clearance by macrophages and protected mice from mortality due to bacterial infection.

View Article: PubMed Central - PubMed

Affiliation: Acupuncture and Moxibustion College, Hubei University of Chinese Medicine, Wuhan, Hubei 430061, China ; Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, Wuhan, Hubei 430061, China.

ABSTRACT
Moxibustion is one of main therapies in traditional Chinese medicine and uses heat stimulation on the body surface from the burning of moxa to release pain or treat diseases. Emerging studies have shown that moxibustion can generate therapeutic effects by activating a series of signaling pathways and neuroendocrine-immune activities. Here we show moxibustion promoted profound macrophage autophagy in experimental Kunming mice, with reduced Akt phosphorylation and activated eIF2α phosphorylation. Consequently, moxibustion promoted bacterial clearance by macrophages and protected mice from mortality due to bacterial infection. These results indicate that moxibustion generates a protective response by activating autophagy against bacterial infections.

No MeSH data available.


Related in: MedlinePlus

Autophagy is accelerated by moxibustion. Macrophages were collected from mice with or without moxibustion as indicated, fixed, and stained with anti-LC3 antibody, and LC3 staining was visualized by confocal fluorescence microscopy. Representative images (a) and the means of LC3 puncta/cell counted from 20 individual cells (b) are shown. **P < 0.01. Macrophages were lysed and equal mixtures of whole cell extracts were detected by western blots with anti-LC3 and anti-actin antibodies (c).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4129972&req=5

fig1: Autophagy is accelerated by moxibustion. Macrophages were collected from mice with or without moxibustion as indicated, fixed, and stained with anti-LC3 antibody, and LC3 staining was visualized by confocal fluorescence microscopy. Representative images (a) and the means of LC3 puncta/cell counted from 20 individual cells (b) are shown. **P < 0.01. Macrophages were lysed and equal mixtures of whole cell extracts were detected by western blots with anti-LC3 and anti-actin antibodies (c).

Mentions: To assess whether moxibustion affected autophagy, moxibustion with a small size moxa stick was applied to the mouse abdomen Guanyuan point (CV4) for 15 and 30 minutes. Macrophages were then isolated from the peritoneal fluid after mice were sacrificed and subjected to autophagy assay. When macrophages were primed by starch and albumen, autophagic LC3-postive vacuoles were not observed in macrophages, similar to that seen in control mice, whereas LC3-positve puncta were greatly increased after 15 min or 30 min moxibustion (Figures 1(a) and 1(b)). Furthermore, when LC3 was detected in cell extracts, the ratio of LC3-II was elevated following moxibustion compared with mock and primed conditions (Figure 1(c)). The levels of LC3-II protein and LC3 puncta were similar under both moxibustion conditions. These results suggest that moxibustion induces the macrophage autophagy in mice.


Moxibustion Activates Macrophage Autophagy and Protects Experimental Mice against Bacterial Infection.

Li X, Guo G, Shen F, Kong L, Liang F, Sun G - Evid Based Complement Alternat Med (2014)

Autophagy is accelerated by moxibustion. Macrophages were collected from mice with or without moxibustion as indicated, fixed, and stained with anti-LC3 antibody, and LC3 staining was visualized by confocal fluorescence microscopy. Representative images (a) and the means of LC3 puncta/cell counted from 20 individual cells (b) are shown. **P < 0.01. Macrophages were lysed and equal mixtures of whole cell extracts were detected by western blots with anti-LC3 and anti-actin antibodies (c).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4129972&req=5

fig1: Autophagy is accelerated by moxibustion. Macrophages were collected from mice with or without moxibustion as indicated, fixed, and stained with anti-LC3 antibody, and LC3 staining was visualized by confocal fluorescence microscopy. Representative images (a) and the means of LC3 puncta/cell counted from 20 individual cells (b) are shown. **P < 0.01. Macrophages were lysed and equal mixtures of whole cell extracts were detected by western blots with anti-LC3 and anti-actin antibodies (c).
Mentions: To assess whether moxibustion affected autophagy, moxibustion with a small size moxa stick was applied to the mouse abdomen Guanyuan point (CV4) for 15 and 30 minutes. Macrophages were then isolated from the peritoneal fluid after mice were sacrificed and subjected to autophagy assay. When macrophages were primed by starch and albumen, autophagic LC3-postive vacuoles were not observed in macrophages, similar to that seen in control mice, whereas LC3-positve puncta were greatly increased after 15 min or 30 min moxibustion (Figures 1(a) and 1(b)). Furthermore, when LC3 was detected in cell extracts, the ratio of LC3-II was elevated following moxibustion compared with mock and primed conditions (Figure 1(c)). The levels of LC3-II protein and LC3 puncta were similar under both moxibustion conditions. These results suggest that moxibustion induces the macrophage autophagy in mice.

Bottom Line: Moxibustion is one of main therapies in traditional Chinese medicine and uses heat stimulation on the body surface from the burning of moxa to release pain or treat diseases.Here we show moxibustion promoted profound macrophage autophagy in experimental Kunming mice, with reduced Akt phosphorylation and activated eIF2α phosphorylation.Consequently, moxibustion promoted bacterial clearance by macrophages and protected mice from mortality due to bacterial infection.

View Article: PubMed Central - PubMed

Affiliation: Acupuncture and Moxibustion College, Hubei University of Chinese Medicine, Wuhan, Hubei 430061, China ; Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, Wuhan, Hubei 430061, China.

ABSTRACT
Moxibustion is one of main therapies in traditional Chinese medicine and uses heat stimulation on the body surface from the burning of moxa to release pain or treat diseases. Emerging studies have shown that moxibustion can generate therapeutic effects by activating a series of signaling pathways and neuroendocrine-immune activities. Here we show moxibustion promoted profound macrophage autophagy in experimental Kunming mice, with reduced Akt phosphorylation and activated eIF2α phosphorylation. Consequently, moxibustion promoted bacterial clearance by macrophages and protected mice from mortality due to bacterial infection. These results indicate that moxibustion generates a protective response by activating autophagy against bacterial infections.

No MeSH data available.


Related in: MedlinePlus