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Peritoneal air exposure elicits an intestinal inflammation resulting in postoperative ileus.

Tan S, Yu W, Lin Z, Chen Q, Shi J, Dong Y, Duan K, Bai X, Xu L, Li J, Li N - Mediators Inflamm. (2014)

Bottom Line: At 24 h after surgery, we analyzed the gastrointestinal transit, the serum levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10, the myeloperoxidase activity, and the levels of TNF-α, IL-1β, IL-6, and IL-10 in the ileum and colon.The oxidant and antioxidant levels in the ileum and colon were analyzed by measuring malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC).Peritoneal air exposure caused an air-exposure-time-dependent decrease in the gastrointestinal transit.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, Nanjing 210002, China.

ABSTRACT

Background: The pathogenesis of postoperative ileus (POI) is complex. The present study was designed to investigate the effects of peritoneal air exposure on the POI intestinal inflammation and the underlying mechanism.

Methods: Sprague-Dawley rats were randomized into five groups (6/group): the control group, the sham group, and three exposure groups with peritoneal air exposure for 1, 2, or 3 h. At 24 h after surgery, we analyzed the gastrointestinal transit, the serum levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10, the myeloperoxidase activity, and the levels of TNF-α, IL-1β, IL-6, and IL-10 in the ileum and colon. The oxidant and antioxidant levels in the ileum and colon were analyzed by measuring malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC).

Results: Peritoneal air exposure caused an air-exposure-time-dependent decrease in the gastrointestinal transit. The length of peritoneal air exposure is correlated with the severity of both systemic and intestinal inflammations and the increases in the levels of MDA, SOD, GSH-Px, and T-AOC.

Conclusions: The length of peritoneal air exposure is proportional to the degree of intestinal paralysis and the severity of intestinal inflammation, which is linked to the oxidative stress response.

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Related in: MedlinePlus

The levels of MDA, SOD, GSH-Px, and T-AOC in the tissues of ileum and colon. CG: control group; SG: sham group; EG1, EG2, and EG3: exposure groups with peritoneal air exposure for 1, 2, and 3 h, respectively. Data are expressed as mean ± SD, n = 8. *P < 0.05, versus the CG group.
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fig6: The levels of MDA, SOD, GSH-Px, and T-AOC in the tissues of ileum and colon. CG: control group; SG: sham group; EG1, EG2, and EG3: exposure groups with peritoneal air exposure for 1, 2, and 3 h, respectively. Data are expressed as mean ± SD, n = 8. *P < 0.05, versus the CG group.

Mentions: As shown in Figure 6, there were no significant differences in the levels of MDA, SOD, GSH-Px, and T-AOC in the tissues of ileum and colon between the control and sham groups. Peritoneal air exposure elicited a progressive increase in the tissue levels of MDA, SOD, GSH-Px, and T-AOC. The ileal MDA activity in the 2 and 3 h exposure groups and the colonic MDA activity in the 3 h exposure group were significantly higher than those of the control group (P < 0.05). The ileal SOD activity in all the three exposure groups and the colonic SOD activity in the 2 and 3 h exposure groups were significantly higher than those of the control group (P < 0.05). For the GSH-Px levels, there were no significant differences between the 1 and 2 h exposure groups and the control group, but the ileal and colonic GSH-Px levels in the 3 h exposure group were significantly higher than those of the control group (P < 0.05). Additionally, the ileal T-AOC levels in the 2 and 3 h exposure groups and the colonic T-AOC level in the 3 h exposure group were significantly higher than those of corresponding control group (P < 0.05).


Peritoneal air exposure elicits an intestinal inflammation resulting in postoperative ileus.

Tan S, Yu W, Lin Z, Chen Q, Shi J, Dong Y, Duan K, Bai X, Xu L, Li J, Li N - Mediators Inflamm. (2014)

The levels of MDA, SOD, GSH-Px, and T-AOC in the tissues of ileum and colon. CG: control group; SG: sham group; EG1, EG2, and EG3: exposure groups with peritoneal air exposure for 1, 2, and 3 h, respectively. Data are expressed as mean ± SD, n = 8. *P < 0.05, versus the CG group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4129966&req=5

fig6: The levels of MDA, SOD, GSH-Px, and T-AOC in the tissues of ileum and colon. CG: control group; SG: sham group; EG1, EG2, and EG3: exposure groups with peritoneal air exposure for 1, 2, and 3 h, respectively. Data are expressed as mean ± SD, n = 8. *P < 0.05, versus the CG group.
Mentions: As shown in Figure 6, there were no significant differences in the levels of MDA, SOD, GSH-Px, and T-AOC in the tissues of ileum and colon between the control and sham groups. Peritoneal air exposure elicited a progressive increase in the tissue levels of MDA, SOD, GSH-Px, and T-AOC. The ileal MDA activity in the 2 and 3 h exposure groups and the colonic MDA activity in the 3 h exposure group were significantly higher than those of the control group (P < 0.05). The ileal SOD activity in all the three exposure groups and the colonic SOD activity in the 2 and 3 h exposure groups were significantly higher than those of the control group (P < 0.05). For the GSH-Px levels, there were no significant differences between the 1 and 2 h exposure groups and the control group, but the ileal and colonic GSH-Px levels in the 3 h exposure group were significantly higher than those of the control group (P < 0.05). Additionally, the ileal T-AOC levels in the 2 and 3 h exposure groups and the colonic T-AOC level in the 3 h exposure group were significantly higher than those of corresponding control group (P < 0.05).

Bottom Line: At 24 h after surgery, we analyzed the gastrointestinal transit, the serum levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10, the myeloperoxidase activity, and the levels of TNF-α, IL-1β, IL-6, and IL-10 in the ileum and colon.The oxidant and antioxidant levels in the ileum and colon were analyzed by measuring malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC).Peritoneal air exposure caused an air-exposure-time-dependent decrease in the gastrointestinal transit.

View Article: PubMed Central - PubMed

Affiliation: Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, Nanjing 210002, China.

ABSTRACT

Background: The pathogenesis of postoperative ileus (POI) is complex. The present study was designed to investigate the effects of peritoneal air exposure on the POI intestinal inflammation and the underlying mechanism.

Methods: Sprague-Dawley rats were randomized into five groups (6/group): the control group, the sham group, and three exposure groups with peritoneal air exposure for 1, 2, or 3 h. At 24 h after surgery, we analyzed the gastrointestinal transit, the serum levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10, the myeloperoxidase activity, and the levels of TNF-α, IL-1β, IL-6, and IL-10 in the ileum and colon. The oxidant and antioxidant levels in the ileum and colon were analyzed by measuring malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC).

Results: Peritoneal air exposure caused an air-exposure-time-dependent decrease in the gastrointestinal transit. The length of peritoneal air exposure is correlated with the severity of both systemic and intestinal inflammations and the increases in the levels of MDA, SOD, GSH-Px, and T-AOC.

Conclusions: The length of peritoneal air exposure is proportional to the degree of intestinal paralysis and the severity of intestinal inflammation, which is linked to the oxidative stress response.

Show MeSH
Related in: MedlinePlus