Limits...
Isolation and characterization of bacteriophages infecting nocardioforms in wastewater treatment plant.

Khairnar K, Pal P, Chandekar RH, Paunikar WN - Biotechnol Res Int (2014)

Bottom Line: Activated sludge plants (ASP) are associated with the stable foaming problem worldwide.This study describes the isolation and characterization of three Nocardia bacteriophages NOC1, NOC2, and NOC3 for the control of Nocardia species.To the best of our knowledge to date no work has been published from India related to biological approach for the control of foaming.

View Article: PubMed Central - PubMed

Affiliation: Environmental Virology Cell, Council of Scientific and Industrial Research, National Environmental Engineering Research Institute (CSIR-NEERI), Nehru Marg, Nagpur, Maharashtra 440020, India.

ABSTRACT
Activated sludge plants (ASP) are associated with the stable foaming problem worldwide. Apart from the physical and chemical treatment methods, biological treatment method has been least explored and may prove to be a novel and ecofriendly approach to tackle the problem of stable foam formation. In ASP Nocardia species are commonly found and are one of the major causes for forming sticky and stable foam. This study describes the isolation and characterization of three Nocardia bacteriophages NOC1, NOC2, and NOC3 for the control of Nocardia species. The bacteriophages isolated in this study have shown promising results in controlling foam producing bacterial growth under laboratory conditions, suggesting that it may prove useful in the field as an alternative biocontrol agent to reduce the foaming problem. To the best of our knowledge to date no work has been published from India related to biological approach for the control of foaming.

No MeSH data available.


Related in: MedlinePlus

Plaque assay of phage; isolated nocardiophage showing clear plaques on ATCC Nocardia bacteria.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4129933&req=5

fig1: Plaque assay of phage; isolated nocardiophage showing clear plaques on ATCC Nocardia bacteria.

Mentions: Petrovski's method was followed for the isolation and purification of bacteriophages. Single plaques were observed after 2 days incubation at 30°C. The quantitative results of plaque assay are summarized in Table 2 indicating phage growth. A representative culture plate of plaque assay is presented, wherein the plaques were picked and subjected to further isolation and purification of bacteriophages (Figure 1). On the basis of growth of bacteriophages on N. rhodochrous, N. amarae, and N. pinensis (reclassified as Skermania piniformis) phages were named as NOC1, NOC2, and NOC3, respectively. Further, plaques were purified by four rounds of dilution and reisolation to ensure that each plaque resulted from a single type of bacteriophage. The plates which were showing the excellent visual growth were taken for the purification of bacteriophages.


Isolation and characterization of bacteriophages infecting nocardioforms in wastewater treatment plant.

Khairnar K, Pal P, Chandekar RH, Paunikar WN - Biotechnol Res Int (2014)

Plaque assay of phage; isolated nocardiophage showing clear plaques on ATCC Nocardia bacteria.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4129933&req=5

fig1: Plaque assay of phage; isolated nocardiophage showing clear plaques on ATCC Nocardia bacteria.
Mentions: Petrovski's method was followed for the isolation and purification of bacteriophages. Single plaques were observed after 2 days incubation at 30°C. The quantitative results of plaque assay are summarized in Table 2 indicating phage growth. A representative culture plate of plaque assay is presented, wherein the plaques were picked and subjected to further isolation and purification of bacteriophages (Figure 1). On the basis of growth of bacteriophages on N. rhodochrous, N. amarae, and N. pinensis (reclassified as Skermania piniformis) phages were named as NOC1, NOC2, and NOC3, respectively. Further, plaques were purified by four rounds of dilution and reisolation to ensure that each plaque resulted from a single type of bacteriophage. The plates which were showing the excellent visual growth were taken for the purification of bacteriophages.

Bottom Line: Activated sludge plants (ASP) are associated with the stable foaming problem worldwide.This study describes the isolation and characterization of three Nocardia bacteriophages NOC1, NOC2, and NOC3 for the control of Nocardia species.To the best of our knowledge to date no work has been published from India related to biological approach for the control of foaming.

View Article: PubMed Central - PubMed

Affiliation: Environmental Virology Cell, Council of Scientific and Industrial Research, National Environmental Engineering Research Institute (CSIR-NEERI), Nehru Marg, Nagpur, Maharashtra 440020, India.

ABSTRACT
Activated sludge plants (ASP) are associated with the stable foaming problem worldwide. Apart from the physical and chemical treatment methods, biological treatment method has been least explored and may prove to be a novel and ecofriendly approach to tackle the problem of stable foam formation. In ASP Nocardia species are commonly found and are one of the major causes for forming sticky and stable foam. This study describes the isolation and characterization of three Nocardia bacteriophages NOC1, NOC2, and NOC3 for the control of Nocardia species. The bacteriophages isolated in this study have shown promising results in controlling foam producing bacterial growth under laboratory conditions, suggesting that it may prove useful in the field as an alternative biocontrol agent to reduce the foaming problem. To the best of our knowledge to date no work has been published from India related to biological approach for the control of foaming.

No MeSH data available.


Related in: MedlinePlus