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Therapeutic effects of smecta or smectite powder on rats with paraquat toxication.

Jiang YS, Ma YY, Wang ZQ, Li GJ - World J Emerg Med (2013)

Bottom Line: Abruption of mucosa, hyperemic gastric mucosa and leukocyte infiltration were obvious in the stomach.The hemorrhage of jejunum mucosa, the abruption of villus, the gland damage with the addition of inflammatory cell infiltration were found.Smecta reduced the plasma concentration of paraquat and alleviated pathologic injury of rats with PQ poisoning.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency Medicine, Affiliated Hospital of Shihezi University, Shihezi 832002, China.

ABSTRACT

Background: The plasma concentration of paraquat is closely related to the prognosis of patients with paraquat toxication, and the most common cause of death from paraquat poisoning is multiple organ failure (MOF). This study aimed to evaluate therapeutic effect of smecta on the plasma concentrations of paraquat and multi-organ injury induced by paraquat intoxication in rats.

Methods: A total of 76 healthy adult SD rats were randomly divided into group A (control group, n=6), group B (poisoned group, n=30) and group C (smecta-treated group, n=30). Rats in groups B and C were treated intragastrically with PQ at 50 mg/kg, and rats in group A was treated intragastrically with saline (1 mL). Rats in group C were given intragastrically smecta at 400 mg/kg 10 minutes after administration of PQ, while rats in other two groups were treated intragastrically with 1 mL saline at the same time. Live rats in groups B and C were sacrificed at 2, 6, 24, 48, 72 hours after administration of PQ for the determination of paraquat plasma concentrations and for HE staining of the lung, stomach and jejunum. The rats were executed at the end of trial by the same way in group A.

Results: The plasma concentration of paraquat (ng/mL) ranged from 440.314±49.776 to 4320.6150±413.947. Distinctive pathological changes were seen in the lung, stomach and jejunum in group B. Lung injuries deteriorated gradually, edema, leukocyte infiltration, pneumorrhagia, incrassated septa and lung consolidation were observed. Abruption of mucosa, hyperemic gastric mucosa and leukocyte infiltration were obvious in the stomach. The hemorrhage of jejunum mucosa, the abruption of villus, the gland damage with the addition of inflammatory cell infiltration were found. Compared to group B, the plasma concentration of paraquat reduced (P<0.01) and the pathological changes mentioned above were obviously alleviated in group C (P<0.05, P<0.01).

Conclusion: Smecta reduced the plasma concentration of paraquat and alleviated pathologic injury of rats with PQ poisoning.

No MeSH data available.


Related in: MedlinePlus

HPLC chromatogram of blank control plasma sample in the control group (PQ concentration, 1000 ng/mL).
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Figure 1: HPLC chromatogram of blank control plasma sample in the control group (PQ concentration, 1000 ng/mL).

Mentions: The regression equation of plasma PQ: Y=98.8210 X +5707.5407. The PQ concentration varied from 20 ng/mL to 5000 ng/mL, and the minimum detection limit was 20 ng/mL. Partial HPLC chromatograms are shown in Figures 1–3.


Therapeutic effects of smecta or smectite powder on rats with paraquat toxication.

Jiang YS, Ma YY, Wang ZQ, Li GJ - World J Emerg Med (2013)

HPLC chromatogram of blank control plasma sample in the control group (PQ concentration, 1000 ng/mL).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4129834&req=5

Figure 1: HPLC chromatogram of blank control plasma sample in the control group (PQ concentration, 1000 ng/mL).
Mentions: The regression equation of plasma PQ: Y=98.8210 X +5707.5407. The PQ concentration varied from 20 ng/mL to 5000 ng/mL, and the minimum detection limit was 20 ng/mL. Partial HPLC chromatograms are shown in Figures 1–3.

Bottom Line: Abruption of mucosa, hyperemic gastric mucosa and leukocyte infiltration were obvious in the stomach.The hemorrhage of jejunum mucosa, the abruption of villus, the gland damage with the addition of inflammatory cell infiltration were found.Smecta reduced the plasma concentration of paraquat and alleviated pathologic injury of rats with PQ poisoning.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency Medicine, Affiliated Hospital of Shihezi University, Shihezi 832002, China.

ABSTRACT

Background: The plasma concentration of paraquat is closely related to the prognosis of patients with paraquat toxication, and the most common cause of death from paraquat poisoning is multiple organ failure (MOF). This study aimed to evaluate therapeutic effect of smecta on the plasma concentrations of paraquat and multi-organ injury induced by paraquat intoxication in rats.

Methods: A total of 76 healthy adult SD rats were randomly divided into group A (control group, n=6), group B (poisoned group, n=30) and group C (smecta-treated group, n=30). Rats in groups B and C were treated intragastrically with PQ at 50 mg/kg, and rats in group A was treated intragastrically with saline (1 mL). Rats in group C were given intragastrically smecta at 400 mg/kg 10 minutes after administration of PQ, while rats in other two groups were treated intragastrically with 1 mL saline at the same time. Live rats in groups B and C were sacrificed at 2, 6, 24, 48, 72 hours after administration of PQ for the determination of paraquat plasma concentrations and for HE staining of the lung, stomach and jejunum. The rats were executed at the end of trial by the same way in group A.

Results: The plasma concentration of paraquat (ng/mL) ranged from 440.314±49.776 to 4320.6150±413.947. Distinctive pathological changes were seen in the lung, stomach and jejunum in group B. Lung injuries deteriorated gradually, edema, leukocyte infiltration, pneumorrhagia, incrassated septa and lung consolidation were observed. Abruption of mucosa, hyperemic gastric mucosa and leukocyte infiltration were obvious in the stomach. The hemorrhage of jejunum mucosa, the abruption of villus, the gland damage with the addition of inflammatory cell infiltration were found. Compared to group B, the plasma concentration of paraquat reduced (P<0.01) and the pathological changes mentioned above were obviously alleviated in group C (P<0.05, P<0.01).

Conclusion: Smecta reduced the plasma concentration of paraquat and alleviated pathologic injury of rats with PQ poisoning.

No MeSH data available.


Related in: MedlinePlus