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Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice.

Verheijden KA, Henricks PA, Redegeld FA, Garssen J, Folkerts G - Front Pharmacol (2014)

Bottom Line: There was a significant increase in the number of inflammatory cells in the Broncho-Alveolar Lavage Fluid (BALF) in both the mild and severe inflammation animals.IL-2 and RANTES levels in the BALF were higher in the severe inflammation groups compared to the mild inflammation groups.Measuring RL gave consistent results in both mild and severe allergic airway inflammation models however, ventilation induced an additional cell influx into the airways.

View Article: PubMed Central - PubMed

Affiliation: Division of Pharmacology, Faculty of Science, Utrecht Institute for Pharmaceutical Sciences, Utrecht University Utrecht, Netherlands.

ABSTRACT
In this study a direct comparison was made between non-invasive and non-ventilated unrestrained whole body plethysmography (Penh) (conscious animals) and the invasive ventilated lung resistance (RL) method (anesthetized animals) in both mild and severe allergic airway inflammation models. Mild inflammation was induced by intraperitoneal sensitization and aerosols of ovalbumin. Severe inflammation was induced by intraperitoneal sensitization using trinitrophenyl-ovalbumin, followed by intranasal challenges with IgE-allergen complexes. A significant increase in airway responsiveness to methacholine was observed in the mild inflammation group when RL was measured. Significant changes in both RL and Penh were observed in the severe inflammation groups. There was a significant increase in the number of inflammatory cells in the Broncho-Alveolar Lavage Fluid (BALF) in both the mild and severe inflammation animals. The enforced ventilation of the animals during the RL measurement further increased the number of cells in the BALF. IL-2 and RANTES levels in the BALF were higher in the severe inflammation groups compared to the mild inflammation groups. Penh gave only reliable measurements during severe airway inflammation. Measuring RL gave consistent results in both mild and severe allergic airway inflammation models however, ventilation induced an additional cell influx into the airways.

No MeSH data available.


Related in: MedlinePlus

Airway responsiveness (Penh) to methacholine in the severe airway inflammation model. Airway responsiveness was measured in mice sensitized with saline or TNP-OVA and challenged intranasally with saline or TNP-OVA-IgE. Values are expressed as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001; using a One-Way ANOVA followed by a Bonferroni post-hoc analysis, n = 7–9 mice/group.
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Figure 4: Airway responsiveness (Penh) to methacholine in the severe airway inflammation model. Airway responsiveness was measured in mice sensitized with saline or TNP-OVA and challenged intranasally with saline or TNP-OVA-IgE. Values are expressed as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001; using a One-Way ANOVA followed by a Bonferroni post-hoc analysis, n = 7–9 mice/group.

Mentions: Basal airway resistance was significantly increased in the TNP-OVA—TNP-OVA-IgE group compared to the SAL-SAL group. The Penh increased dose-dependently in the SAL-SAL group after methacholine inhalation (Figure 4). The Penh in the TNP-OVA—TNP-OVA-IgE group was significantly increased compared to the SAL-SAL group to a maximum of 12.33 ± 1.99 (Figure 4). The increase in Penh was 1.5 times higher compared to the sensitized and challenged animals with mild airway inflammation (Figure 2). Although the basal RL did not differ in the OVA-OVA group of the mild model (Figure 3), basal RL tended to increase in the severe model (Figure 5), reaching the level of significance after saline exposure. As in the mild model, methacholine slightly increased the RL in the SAL-SAL group of the severe model. The RL was significantly increased in the TNP-OVA—TNP-OVA-IgE group after the methacholine inhalation to a maximum of 1.76 ± 0.18 cm H2O/mL*s−1. The maximal increase in RL to methacholine in the severe model was a level similar to the OVA-OVA group in the mild airway inflammation model, but the sensitivity was higher as evidenced by significant changes at lower doses of methacholine (Figures 3, 5).


Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice.

Verheijden KA, Henricks PA, Redegeld FA, Garssen J, Folkerts G - Front Pharmacol (2014)

Airway responsiveness (Penh) to methacholine in the severe airway inflammation model. Airway responsiveness was measured in mice sensitized with saline or TNP-OVA and challenged intranasally with saline or TNP-OVA-IgE. Values are expressed as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001; using a One-Way ANOVA followed by a Bonferroni post-hoc analysis, n = 7–9 mice/group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4129625&req=5

Figure 4: Airway responsiveness (Penh) to methacholine in the severe airway inflammation model. Airway responsiveness was measured in mice sensitized with saline or TNP-OVA and challenged intranasally with saline or TNP-OVA-IgE. Values are expressed as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001; using a One-Way ANOVA followed by a Bonferroni post-hoc analysis, n = 7–9 mice/group.
Mentions: Basal airway resistance was significantly increased in the TNP-OVA—TNP-OVA-IgE group compared to the SAL-SAL group. The Penh increased dose-dependently in the SAL-SAL group after methacholine inhalation (Figure 4). The Penh in the TNP-OVA—TNP-OVA-IgE group was significantly increased compared to the SAL-SAL group to a maximum of 12.33 ± 1.99 (Figure 4). The increase in Penh was 1.5 times higher compared to the sensitized and challenged animals with mild airway inflammation (Figure 2). Although the basal RL did not differ in the OVA-OVA group of the mild model (Figure 3), basal RL tended to increase in the severe model (Figure 5), reaching the level of significance after saline exposure. As in the mild model, methacholine slightly increased the RL in the SAL-SAL group of the severe model. The RL was significantly increased in the TNP-OVA—TNP-OVA-IgE group after the methacholine inhalation to a maximum of 1.76 ± 0.18 cm H2O/mL*s−1. The maximal increase in RL to methacholine in the severe model was a level similar to the OVA-OVA group in the mild airway inflammation model, but the sensitivity was higher as evidenced by significant changes at lower doses of methacholine (Figures 3, 5).

Bottom Line: There was a significant increase in the number of inflammatory cells in the Broncho-Alveolar Lavage Fluid (BALF) in both the mild and severe inflammation animals.IL-2 and RANTES levels in the BALF were higher in the severe inflammation groups compared to the mild inflammation groups.Measuring RL gave consistent results in both mild and severe allergic airway inflammation models however, ventilation induced an additional cell influx into the airways.

View Article: PubMed Central - PubMed

Affiliation: Division of Pharmacology, Faculty of Science, Utrecht Institute for Pharmaceutical Sciences, Utrecht University Utrecht, Netherlands.

ABSTRACT
In this study a direct comparison was made between non-invasive and non-ventilated unrestrained whole body plethysmography (Penh) (conscious animals) and the invasive ventilated lung resistance (RL) method (anesthetized animals) in both mild and severe allergic airway inflammation models. Mild inflammation was induced by intraperitoneal sensitization and aerosols of ovalbumin. Severe inflammation was induced by intraperitoneal sensitization using trinitrophenyl-ovalbumin, followed by intranasal challenges with IgE-allergen complexes. A significant increase in airway responsiveness to methacholine was observed in the mild inflammation group when RL was measured. Significant changes in both RL and Penh were observed in the severe inflammation groups. There was a significant increase in the number of inflammatory cells in the Broncho-Alveolar Lavage Fluid (BALF) in both the mild and severe inflammation animals. The enforced ventilation of the animals during the RL measurement further increased the number of cells in the BALF. IL-2 and RANTES levels in the BALF were higher in the severe inflammation groups compared to the mild inflammation groups. Penh gave only reliable measurements during severe airway inflammation. Measuring RL gave consistent results in both mild and severe allergic airway inflammation models however, ventilation induced an additional cell influx into the airways.

No MeSH data available.


Related in: MedlinePlus