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Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice.

Verheijden KA, Henricks PA, Redegeld FA, Garssen J, Folkerts G - Front Pharmacol (2014)

Bottom Line: There was a significant increase in the number of inflammatory cells in the Broncho-Alveolar Lavage Fluid (BALF) in both the mild and severe inflammation animals.IL-2 and RANTES levels in the BALF were higher in the severe inflammation groups compared to the mild inflammation groups.Measuring RL gave consistent results in both mild and severe allergic airway inflammation models however, ventilation induced an additional cell influx into the airways.

View Article: PubMed Central - PubMed

Affiliation: Division of Pharmacology, Faculty of Science, Utrecht Institute for Pharmaceutical Sciences, Utrecht University Utrecht, Netherlands.

ABSTRACT
In this study a direct comparison was made between non-invasive and non-ventilated unrestrained whole body plethysmography (Penh) (conscious animals) and the invasive ventilated lung resistance (RL) method (anesthetized animals) in both mild and severe allergic airway inflammation models. Mild inflammation was induced by intraperitoneal sensitization and aerosols of ovalbumin. Severe inflammation was induced by intraperitoneal sensitization using trinitrophenyl-ovalbumin, followed by intranasal challenges with IgE-allergen complexes. A significant increase in airway responsiveness to methacholine was observed in the mild inflammation group when RL was measured. Significant changes in both RL and Penh were observed in the severe inflammation groups. There was a significant increase in the number of inflammatory cells in the Broncho-Alveolar Lavage Fluid (BALF) in both the mild and severe inflammation animals. The enforced ventilation of the animals during the RL measurement further increased the number of cells in the BALF. IL-2 and RANTES levels in the BALF were higher in the severe inflammation groups compared to the mild inflammation groups. Penh gave only reliable measurements during severe airway inflammation. Measuring RL gave consistent results in both mild and severe allergic airway inflammation models however, ventilation induced an additional cell influx into the airways.

No MeSH data available.


Related in: MedlinePlus

Experimental scheme of the mild airway inflammation model (A) and severe airway inflammation model (B).
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Figure 1: Experimental scheme of the mild airway inflammation model (A) and severe airway inflammation model (B).

Mentions: On days 0 and 7 mice were sensitized with ovalbumin (OVA; chicken egg albumin, grade V, Sigma, St. Louis, MO, USA) or treated with saline. Active sensitization was conducted by two intraperitoneal injections of 0.1 mL alum-precipitated antigen, comprising 10 μ g OVA absorbed into 2.25 mg alum (AlumImject; Pierce, Rockford, IL, USA). On days 35, 38, and 41 mice were exposed either to an OVA (1% ovalbumin in pyrogen-free saline, OVA group) or control solution (saline, SAL group) aerosol challenge for 30 min. The aerosol was conducted in a plexiglass exposure chamber (5 L) coupled to a Pari LC Star nebulizer (PARI Respiratory Equipment, Richmond, VA, USA; particle size 2.5–3.1 μm) driven by compressed air at a flow rate of 6 L/min (Ten Broeke et al., 2006) (Figure 1A). An overview of the groups included in this study is given in Table 1.


Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice.

Verheijden KA, Henricks PA, Redegeld FA, Garssen J, Folkerts G - Front Pharmacol (2014)

Experimental scheme of the mild airway inflammation model (A) and severe airway inflammation model (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4129625&req=5

Figure 1: Experimental scheme of the mild airway inflammation model (A) and severe airway inflammation model (B).
Mentions: On days 0 and 7 mice were sensitized with ovalbumin (OVA; chicken egg albumin, grade V, Sigma, St. Louis, MO, USA) or treated with saline. Active sensitization was conducted by two intraperitoneal injections of 0.1 mL alum-precipitated antigen, comprising 10 μ g OVA absorbed into 2.25 mg alum (AlumImject; Pierce, Rockford, IL, USA). On days 35, 38, and 41 mice were exposed either to an OVA (1% ovalbumin in pyrogen-free saline, OVA group) or control solution (saline, SAL group) aerosol challenge for 30 min. The aerosol was conducted in a plexiglass exposure chamber (5 L) coupled to a Pari LC Star nebulizer (PARI Respiratory Equipment, Richmond, VA, USA; particle size 2.5–3.1 μm) driven by compressed air at a flow rate of 6 L/min (Ten Broeke et al., 2006) (Figure 1A). An overview of the groups included in this study is given in Table 1.

Bottom Line: There was a significant increase in the number of inflammatory cells in the Broncho-Alveolar Lavage Fluid (BALF) in both the mild and severe inflammation animals.IL-2 and RANTES levels in the BALF were higher in the severe inflammation groups compared to the mild inflammation groups.Measuring RL gave consistent results in both mild and severe allergic airway inflammation models however, ventilation induced an additional cell influx into the airways.

View Article: PubMed Central - PubMed

Affiliation: Division of Pharmacology, Faculty of Science, Utrecht Institute for Pharmaceutical Sciences, Utrecht University Utrecht, Netherlands.

ABSTRACT
In this study a direct comparison was made between non-invasive and non-ventilated unrestrained whole body plethysmography (Penh) (conscious animals) and the invasive ventilated lung resistance (RL) method (anesthetized animals) in both mild and severe allergic airway inflammation models. Mild inflammation was induced by intraperitoneal sensitization and aerosols of ovalbumin. Severe inflammation was induced by intraperitoneal sensitization using trinitrophenyl-ovalbumin, followed by intranasal challenges with IgE-allergen complexes. A significant increase in airway responsiveness to methacholine was observed in the mild inflammation group when RL was measured. Significant changes in both RL and Penh were observed in the severe inflammation groups. There was a significant increase in the number of inflammatory cells in the Broncho-Alveolar Lavage Fluid (BALF) in both the mild and severe inflammation animals. The enforced ventilation of the animals during the RL measurement further increased the number of cells in the BALF. IL-2 and RANTES levels in the BALF were higher in the severe inflammation groups compared to the mild inflammation groups. Penh gave only reliable measurements during severe airway inflammation. Measuring RL gave consistent results in both mild and severe allergic airway inflammation models however, ventilation induced an additional cell influx into the airways.

No MeSH data available.


Related in: MedlinePlus