Limits...
Usage patterns of biomarkers in non-small-cell lung cancer patients in India: Findings from a systematic review and survey.

Desai C, Mehta A, Mishra D - Lung India (2014)

Bottom Line: Prognostic or predictive role of cytokines and angiogenic markers as well as DNA expression were evaluated.The survey also showed that IHC was the most common technique for detection of biomarkers.This systematic review and survey provides valuable information on biomarker usage in the Indian population, and highlights the need for initiatives required for future biomarker testing in India.

View Article: PubMed Central - PubMed

Affiliation: Department of Hemato-Oncology Clinic, Vedanta Institute of Medical Sciences, Ahmedabad, Gujarat, India.

ABSTRACT

Introduction: Personalized medicine has facilitated improved management of non-small cell lung cancer (NSCLC) patients by identifying predictive and prognostic biomarkers for enhanced efficiency of detection and efficacy of treatment. This systematic review and survey assessed the patterns of biomarker usage, molecular testing techniques to diagnose patients with NSCLC in India and testing techniques recommended by cancer societies.

Materials and methods: Studies were retrieved from Embase, PubMed, and Cochrane databases for the last 12 years, using relevant search strategies as per the Cochrane methodology for systematic reviews. Outcomes of interest were biomarkers for NSCLC, patterns of biomarker testing, diagnostic methods, guidelines and cost of biomarker testing.

Results: In all, 499 studies were identified for screening and 17 primary publications were included in the review. Epidermal growth factor receptor (EGFR) expression and epithelial markers (particularly cytokeratins (CK)) were the most commonly reported biomarkers (7/17) and immunohistochemical (IHC) staining was the most common technique for detection of biomarkers. The frequency of EGFR mutations was higher among women than men. Significantly elevated levels of CK-18 were observed in patients with squamous cell carcinoma and of CK-19 in patients with adenocarcinoma, squamous cell carcinoma, and NSCLC (P < 0.001). Prognostic or predictive role of cytokines and angiogenic markers as well as DNA expression were evaluated. The survey also showed that IHC was the most common technique for detection of biomarkers.

Conclusions: This systematic review and survey provides valuable information on biomarker usage in the Indian population, and highlights the need for initiatives required for future biomarker testing in India.

No MeSH data available.


Related in: MedlinePlus

Testing techniques for detection of biomarkers. FISH: fluorescence in situ hybdridization; IHC: Immunohistochemical; PCR: Polymerase chain reaction
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4129597&req=5

Figure 3: Testing techniques for detection of biomarkers. FISH: fluorescence in situ hybdridization; IHC: Immunohistochemical; PCR: Polymerase chain reaction

Mentions: In total, 75 respondents provided information regarding general perception on the usage of biomarkers in India. The survey results identified highest responders for the usage of biomarkers as ‘rarely/sometime’, followed by ‘aware but never use’ for EGFR, KRAS and ALK biomarkers [Figure 2]. For the testing techniques (N = 50), IHC was used as the commonly used technique as per the survey followed by fluorescence in situ hybridization (FISH) [Figure 3]. This was irrespective of the biomarker being tested.


Usage patterns of biomarkers in non-small-cell lung cancer patients in India: Findings from a systematic review and survey.

Desai C, Mehta A, Mishra D - Lung India (2014)

Testing techniques for detection of biomarkers. FISH: fluorescence in situ hybdridization; IHC: Immunohistochemical; PCR: Polymerase chain reaction
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4129597&req=5

Figure 3: Testing techniques for detection of biomarkers. FISH: fluorescence in situ hybdridization; IHC: Immunohistochemical; PCR: Polymerase chain reaction
Mentions: In total, 75 respondents provided information regarding general perception on the usage of biomarkers in India. The survey results identified highest responders for the usage of biomarkers as ‘rarely/sometime’, followed by ‘aware but never use’ for EGFR, KRAS and ALK biomarkers [Figure 2]. For the testing techniques (N = 50), IHC was used as the commonly used technique as per the survey followed by fluorescence in situ hybridization (FISH) [Figure 3]. This was irrespective of the biomarker being tested.

Bottom Line: Prognostic or predictive role of cytokines and angiogenic markers as well as DNA expression were evaluated.The survey also showed that IHC was the most common technique for detection of biomarkers.This systematic review and survey provides valuable information on biomarker usage in the Indian population, and highlights the need for initiatives required for future biomarker testing in India.

View Article: PubMed Central - PubMed

Affiliation: Department of Hemato-Oncology Clinic, Vedanta Institute of Medical Sciences, Ahmedabad, Gujarat, India.

ABSTRACT

Introduction: Personalized medicine has facilitated improved management of non-small cell lung cancer (NSCLC) patients by identifying predictive and prognostic biomarkers for enhanced efficiency of detection and efficacy of treatment. This systematic review and survey assessed the patterns of biomarker usage, molecular testing techniques to diagnose patients with NSCLC in India and testing techniques recommended by cancer societies.

Materials and methods: Studies were retrieved from Embase, PubMed, and Cochrane databases for the last 12 years, using relevant search strategies as per the Cochrane methodology for systematic reviews. Outcomes of interest were biomarkers for NSCLC, patterns of biomarker testing, diagnostic methods, guidelines and cost of biomarker testing.

Results: In all, 499 studies were identified for screening and 17 primary publications were included in the review. Epidermal growth factor receptor (EGFR) expression and epithelial markers (particularly cytokeratins (CK)) were the most commonly reported biomarkers (7/17) and immunohistochemical (IHC) staining was the most common technique for detection of biomarkers. The frequency of EGFR mutations was higher among women than men. Significantly elevated levels of CK-18 were observed in patients with squamous cell carcinoma and of CK-19 in patients with adenocarcinoma, squamous cell carcinoma, and NSCLC (P < 0.001). Prognostic or predictive role of cytokines and angiogenic markers as well as DNA expression were evaluated. The survey also showed that IHC was the most common technique for detection of biomarkers.

Conclusions: This systematic review and survey provides valuable information on biomarker usage in the Indian population, and highlights the need for initiatives required for future biomarker testing in India.

No MeSH data available.


Related in: MedlinePlus