Limits...
Quantitative proteomic analysis reveals posttranslational responses to aneuploidy in yeast.

Dephoure N, Hwang S, O'Sullivan C, Dodgson SE, Gygi SP, Amon A, Torres EM - Elife (2014)

Bottom Line: Our proteomic analyses further revealed a novel aneuploidy-associated protein expression signature characteristic of altered metabolism and redox homeostasis.Interestingly, increased protein turnover attenuates ROS levels and this novel aneuploidy-associated signature and improves the fitness of most aneuploid strains.Our results show that aneuploidy causes alterations in metabolism and redox homeostasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology, Harvard Medical School, Boston, United States.

Show MeSH

Related in: MedlinePlus

ESR, ribosome and proteasome levels in disomes-ubp6Δ.(A) Comparison of transcript (left) and protein levels (right) of the ESR of disomes-ubp6Δ relative to wild-type cells grown in YEPD medium. Down and upregulated genes are defined as in Gasch et al. (2000). (B) Averaged protein levels of ribosome (top) and proteasome subunits (bottom) of each disome-ubp6Δ relative to wild-type cells grown in YEPD medium. Error bars represent SEM.DOI:http://dx.doi.org/10.7554/eLife.03023.036
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4129440&req=5

fig8s2: ESR, ribosome and proteasome levels in disomes-ubp6Δ.(A) Comparison of transcript (left) and protein levels (right) of the ESR of disomes-ubp6Δ relative to wild-type cells grown in YEPD medium. Down and upregulated genes are defined as in Gasch et al. (2000). (B) Averaged protein levels of ribosome (top) and proteasome subunits (bottom) of each disome-ubp6Δ relative to wild-type cells grown in YEPD medium. Error bars represent SEM.DOI:http://dx.doi.org/10.7554/eLife.03023.036

Mentions: Consistent with oxidative stress responsive protein levels being upregulated in aneuploid cells and attenuated upon loss of UBP6, we found that the APS was significantly reduced in the disomic yeast strains lacking UBP6 (Figure 8A). Interestingly, disome V, whose fitness is significantly improved upon deletion of UBP6, showed the strongest reduction in the APS. In contrast, we found that the ESR was not significantly affected in the disomic strains lacking UBP6 (Figure 8—figure supplement 2). This is consistent with the fact that only 2 disomes, disome V and XI, show significant improvements in fitness when grown in rich medium (Torres et al., 2010). Our results indicate that loss of UBP6 ameliorates protein responses associated with altered redox homeostasis and metabolism.10.7554/eLife.03023.033Figure 8.Loss of UBP6 attenuates cellular responses to aneuploidy.


Quantitative proteomic analysis reveals posttranslational responses to aneuploidy in yeast.

Dephoure N, Hwang S, O'Sullivan C, Dodgson SE, Gygi SP, Amon A, Torres EM - Elife (2014)

ESR, ribosome and proteasome levels in disomes-ubp6Δ.(A) Comparison of transcript (left) and protein levels (right) of the ESR of disomes-ubp6Δ relative to wild-type cells grown in YEPD medium. Down and upregulated genes are defined as in Gasch et al. (2000). (B) Averaged protein levels of ribosome (top) and proteasome subunits (bottom) of each disome-ubp6Δ relative to wild-type cells grown in YEPD medium. Error bars represent SEM.DOI:http://dx.doi.org/10.7554/eLife.03023.036
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4129440&req=5

fig8s2: ESR, ribosome and proteasome levels in disomes-ubp6Δ.(A) Comparison of transcript (left) and protein levels (right) of the ESR of disomes-ubp6Δ relative to wild-type cells grown in YEPD medium. Down and upregulated genes are defined as in Gasch et al. (2000). (B) Averaged protein levels of ribosome (top) and proteasome subunits (bottom) of each disome-ubp6Δ relative to wild-type cells grown in YEPD medium. Error bars represent SEM.DOI:http://dx.doi.org/10.7554/eLife.03023.036
Mentions: Consistent with oxidative stress responsive protein levels being upregulated in aneuploid cells and attenuated upon loss of UBP6, we found that the APS was significantly reduced in the disomic yeast strains lacking UBP6 (Figure 8A). Interestingly, disome V, whose fitness is significantly improved upon deletion of UBP6, showed the strongest reduction in the APS. In contrast, we found that the ESR was not significantly affected in the disomic strains lacking UBP6 (Figure 8—figure supplement 2). This is consistent with the fact that only 2 disomes, disome V and XI, show significant improvements in fitness when grown in rich medium (Torres et al., 2010). Our results indicate that loss of UBP6 ameliorates protein responses associated with altered redox homeostasis and metabolism.10.7554/eLife.03023.033Figure 8.Loss of UBP6 attenuates cellular responses to aneuploidy.

Bottom Line: Our proteomic analyses further revealed a novel aneuploidy-associated protein expression signature characteristic of altered metabolism and redox homeostasis.Interestingly, increased protein turnover attenuates ROS levels and this novel aneuploidy-associated signature and improves the fitness of most aneuploid strains.Our results show that aneuploidy causes alterations in metabolism and redox homeostasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology, Harvard Medical School, Boston, United States.

Show MeSH
Related in: MedlinePlus