Quantitative proteomic analysis reveals posttranslational responses to aneuploidy in yeast.
Bottom Line: Our proteomic analyses further revealed a novel aneuploidy-associated protein expression signature characteristic of altered metabolism and redox homeostasis.Interestingly, increased protein turnover attenuates ROS levels and this novel aneuploidy-associated signature and improves the fitness of most aneuploid strains.Our results show that aneuploidy causes alterations in metabolism and redox homeostasis.
Affiliation: Department of Cell Biology, Harvard Medical School, Boston, United States.Show MeSH
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Mentions: Consistent with oxidative stress responsive protein levels being upregulated in aneuploid cells and attenuated upon loss of UBP6, we found that the APS was significantly reduced in the disomic yeast strains lacking UBP6 (Figure 8A). Interestingly, disome V, whose fitness is significantly improved upon deletion of UBP6, showed the strongest reduction in the APS. In contrast, we found that the ESR was not significantly affected in the disomic strains lacking UBP6 (Figure 8—figure supplement 2). This is consistent with the fact that only 2 disomes, disome V and XI, show significant improvements in fitness when grown in rich medium (Torres et al., 2010). Our results indicate that loss of UBP6 ameliorates protein responses associated with altered redox homeostasis and metabolism.10.7554/eLife.03023.033Figure 8.Loss of UBP6 attenuates cellular responses to aneuploidy.
Affiliation: Department of Cell Biology, Harvard Medical School, Boston, United States.