Quantitative proteomic analysis reveals posttranslational responses to aneuploidy in yeast.
Bottom Line: Our proteomic analyses further revealed a novel aneuploidy-associated protein expression signature characteristic of altered metabolism and redox homeostasis.Interestingly, increased protein turnover attenuates ROS levels and this novel aneuploidy-associated signature and improves the fitness of most aneuploid strains.Our results show that aneuploidy causes alterations in metabolism and redox homeostasis.
Affiliation: Department of Cell Biology, Harvard Medical School, Boston, United States.Show MeSH
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Mentions: To determine the mechanisms that prevent an increase in protein levels despite increased gene dosage, we compared mRNA and protein levels of the attenuated genes in disomes grown in rich medium. Transcript levels of the attenuated proteins showed increases close to twofold and, unlike their protein products, showed no signs of compensation (Figure 3A). Strikingly, ribosomal genes encoded on duplicated chromosomes showed mean increases close to twofold in their mRNA levels while every ribosomal protein exhibited attenuation (Figure 3B). Similar results were obtained with cells grown in selective medium and analyzed by SILAC (Figure 3—figure supplement 2).10.7554/eLife.03023.012Figure 3.Attenuation takes place posttranslationally.
Affiliation: Department of Cell Biology, Harvard Medical School, Boston, United States.