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Transformation of membrane nanosurface of red blood cells under hemin action.

Kozlova E, Chernysh A, Moroz V, Gudkova O, Sergunova V, Kuzovlev A - Sci Rep (2014)

Bottom Line: The process of formation of "grains" was dependent on the hemin concentration and incubation time.The possible mechanism of membrane nanostructure alterations is proposed.This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.

View Article: PubMed Central - PubMed

Affiliation: V.A. Negovsky Scientific Research Institute of General Reanimatology RAS, Moscow, Russian Federation.

ABSTRACT
Hemin is the product of hemoglobin oxidation. Some diseases may lead to a formation of hemin. The accumulation of hemin causes destruction of red blood cells (RBC) membranes. In this study the process of development of topological defects of RBC membranes within the size range from nanoscale to microscale levels is shown. The formation of the grain-like structures in the membrane ("grains") with typical sizes of 120-200 nm was experimentally shown. The process of formation of "grains" was dependent on the hemin concentration and incubation time. The possible mechanism of membrane nanostructure alterations is proposed. The kinetic equations of formation and transformation of small and medium topological defects were analyzed. This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.

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Related in: MedlinePlus

Fragment of nanosurface of control cell, C = 0.(a) AFM 3D- image of membrane nanosurface fragment 900 × 900 nm. (b) Model of profile. (c) AFM- profile in the adjusted section on the surface. Arrows show images of protein complexes in a model. Membrane nanoscructure and profiles are represented as typical for given concentration S = 0 among 108 areas.
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f7: Fragment of nanosurface of control cell, C = 0.(a) AFM 3D- image of membrane nanosurface fragment 900 × 900 nm. (b) Model of profile. (c) AFM- profile in the adjusted section on the surface. Arrows show images of protein complexes in a model. Membrane nanoscructure and profiles are represented as typical for given concentration S = 0 among 108 areas.

Mentions: In Fig. 7a the scanning image of the control membrane (no hemin, 800 × 800 nm) is shown. On this image the cantilever delineates the protein complexes, i.e. peaks and cavities on the cell membrane which correspond to the junctional complexes in a lipid membrane. The maximum contour length of the spectrin tetramer is estimated to be 200 nm21. However, the end-to-end distance of the tetramer was estimated to be 70 nm21. AFM images of RBCs under the physiological conditions showed the spectrin tetramer to be in a compressed state in the network, with an average length between 35 to 100 nm22. These findings indicated that in the resting state of RBC the average extension of the tetramer is only a fraction of its contour length21. Fig. 7 demonstrates an AFM image of a control cell surface (Fig. 7a), scheme of ankyrin complex (red-rose) and 4.1R complex (cyan-blue) (Fig. 7b) and the space profile (Fig. 7c) corresponding to the scheme and AFM image. The typical distance between maximum and minimum heights on the profile is 1.2 ± 0.8 nm, and the space period is L = 80 ± 20 nm.


Transformation of membrane nanosurface of red blood cells under hemin action.

Kozlova E, Chernysh A, Moroz V, Gudkova O, Sergunova V, Kuzovlev A - Sci Rep (2014)

Fragment of nanosurface of control cell, C = 0.(a) AFM 3D- image of membrane nanosurface fragment 900 × 900 nm. (b) Model of profile. (c) AFM- profile in the adjusted section on the surface. Arrows show images of protein complexes in a model. Membrane nanoscructure and profiles are represented as typical for given concentration S = 0 among 108 areas.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4129419&req=5

f7: Fragment of nanosurface of control cell, C = 0.(a) AFM 3D- image of membrane nanosurface fragment 900 × 900 nm. (b) Model of profile. (c) AFM- profile in the adjusted section on the surface. Arrows show images of protein complexes in a model. Membrane nanoscructure and profiles are represented as typical for given concentration S = 0 among 108 areas.
Mentions: In Fig. 7a the scanning image of the control membrane (no hemin, 800 × 800 nm) is shown. On this image the cantilever delineates the protein complexes, i.e. peaks and cavities on the cell membrane which correspond to the junctional complexes in a lipid membrane. The maximum contour length of the spectrin tetramer is estimated to be 200 nm21. However, the end-to-end distance of the tetramer was estimated to be 70 nm21. AFM images of RBCs under the physiological conditions showed the spectrin tetramer to be in a compressed state in the network, with an average length between 35 to 100 nm22. These findings indicated that in the resting state of RBC the average extension of the tetramer is only a fraction of its contour length21. Fig. 7 demonstrates an AFM image of a control cell surface (Fig. 7a), scheme of ankyrin complex (red-rose) and 4.1R complex (cyan-blue) (Fig. 7b) and the space profile (Fig. 7c) corresponding to the scheme and AFM image. The typical distance between maximum and minimum heights on the profile is 1.2 ± 0.8 nm, and the space period is L = 80 ± 20 nm.

Bottom Line: The process of formation of "grains" was dependent on the hemin concentration and incubation time.The possible mechanism of membrane nanostructure alterations is proposed.This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.

View Article: PubMed Central - PubMed

Affiliation: V.A. Negovsky Scientific Research Institute of General Reanimatology RAS, Moscow, Russian Federation.

ABSTRACT
Hemin is the product of hemoglobin oxidation. Some diseases may lead to a formation of hemin. The accumulation of hemin causes destruction of red blood cells (RBC) membranes. In this study the process of development of topological defects of RBC membranes within the size range from nanoscale to microscale levels is shown. The formation of the grain-like structures in the membrane ("grains") with typical sizes of 120-200 nm was experimentally shown. The process of formation of "grains" was dependent on the hemin concentration and incubation time. The possible mechanism of membrane nanostructure alterations is proposed. The kinetic equations of formation and transformation of small and medium topological defects were analyzed. This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.

Show MeSH
Related in: MedlinePlus