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Transformation of membrane nanosurface of red blood cells under hemin action.

Kozlova E, Chernysh A, Moroz V, Gudkova O, Sergunova V, Kuzovlev A - Sci Rep (2014)

Bottom Line: The process of formation of "grains" was dependent on the hemin concentration and incubation time.The possible mechanism of membrane nanostructure alterations is proposed.This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.

View Article: PubMed Central - PubMed

Affiliation: V.A. Negovsky Scientific Research Institute of General Reanimatology RAS, Moscow, Russian Federation.

ABSTRACT
Hemin is the product of hemoglobin oxidation. Some diseases may lead to a formation of hemin. The accumulation of hemin causes destruction of red blood cells (RBC) membranes. In this study the process of development of topological defects of RBC membranes within the size range from nanoscale to microscale levels is shown. The formation of the grain-like structures in the membrane ("grains") with typical sizes of 120-200 nm was experimentally shown. The process of formation of "grains" was dependent on the hemin concentration and incubation time. The possible mechanism of membrane nanostructure alterations is proposed. The kinetic equations of formation and transformation of small and medium topological defects were analyzed. This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.

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Related in: MedlinePlus

Merging of “grains” in the domain.(a) RBC 3D- image – in its membrane partial merging of “grains” in a domain occurred (arrows). (b) 3D-image of the fragment of membrane surface with merged “grains” in domain (arrows). (c) Profile of this surface. Interaction time of hemin with RBCs was 1 hour, C = 2.5 mM. Cells, membrane nanoscructure and profiles are represented as representative for given concentration and incubation time among 3120 cells.
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f6: Merging of “grains” in the domain.(a) RBC 3D- image – in its membrane partial merging of “grains” in a domain occurred (arrows). (b) 3D-image of the fragment of membrane surface with merged “grains” in domain (arrows). (c) Profile of this surface. Interaction time of hemin with RBCs was 1 hour, C = 2.5 mM. Cells, membrane nanoscructure and profiles are represented as representative for given concentration and incubation time among 3120 cells.

Mentions: The fusion of “grains” within the domains and domains with each other was detected when the hemin concentration was increased. The microscale characteristics of the fused topological patterns are Lf = 900 ± 100 nm (Fig. 6). Separate and fused “grains” were simultaneously observed on the membrane surface (Fig. 6 a,b). The profile of the membrane surface in this case (Fig. 6c) was differed from that with “grains” (Fig. 3c).


Transformation of membrane nanosurface of red blood cells under hemin action.

Kozlova E, Chernysh A, Moroz V, Gudkova O, Sergunova V, Kuzovlev A - Sci Rep (2014)

Merging of “grains” in the domain.(a) RBC 3D- image – in its membrane partial merging of “grains” in a domain occurred (arrows). (b) 3D-image of the fragment of membrane surface with merged “grains” in domain (arrows). (c) Profile of this surface. Interaction time of hemin with RBCs was 1 hour, C = 2.5 mM. Cells, membrane nanoscructure and profiles are represented as representative for given concentration and incubation time among 3120 cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4129419&req=5

f6: Merging of “grains” in the domain.(a) RBC 3D- image – in its membrane partial merging of “grains” in a domain occurred (arrows). (b) 3D-image of the fragment of membrane surface with merged “grains” in domain (arrows). (c) Profile of this surface. Interaction time of hemin with RBCs was 1 hour, C = 2.5 mM. Cells, membrane nanoscructure and profiles are represented as representative for given concentration and incubation time among 3120 cells.
Mentions: The fusion of “grains” within the domains and domains with each other was detected when the hemin concentration was increased. The microscale characteristics of the fused topological patterns are Lf = 900 ± 100 nm (Fig. 6). Separate and fused “grains” were simultaneously observed on the membrane surface (Fig. 6 a,b). The profile of the membrane surface in this case (Fig. 6c) was differed from that with “grains” (Fig. 3c).

Bottom Line: The process of formation of "grains" was dependent on the hemin concentration and incubation time.The possible mechanism of membrane nanostructure alterations is proposed.This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.

View Article: PubMed Central - PubMed

Affiliation: V.A. Negovsky Scientific Research Institute of General Reanimatology RAS, Moscow, Russian Federation.

ABSTRACT
Hemin is the product of hemoglobin oxidation. Some diseases may lead to a formation of hemin. The accumulation of hemin causes destruction of red blood cells (RBC) membranes. In this study the process of development of topological defects of RBC membranes within the size range from nanoscale to microscale levels is shown. The formation of the grain-like structures in the membrane ("grains") with typical sizes of 120-200 nm was experimentally shown. The process of formation of "grains" was dependent on the hemin concentration and incubation time. The possible mechanism of membrane nanostructure alterations is proposed. The kinetic equations of formation and transformation of small and medium topological defects were analyzed. This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.

Show MeSH
Related in: MedlinePlus