Limits...
Transformation of membrane nanosurface of red blood cells under hemin action.

Kozlova E, Chernysh A, Moroz V, Gudkova O, Sergunova V, Kuzovlev A - Sci Rep (2014)

Bottom Line: The process of formation of "grains" was dependent on the hemin concentration and incubation time.The possible mechanism of membrane nanostructure alterations is proposed.This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.

View Article: PubMed Central - PubMed

Affiliation: V.A. Negovsky Scientific Research Institute of General Reanimatology RAS, Moscow, Russian Federation.

ABSTRACT
Hemin is the product of hemoglobin oxidation. Some diseases may lead to a formation of hemin. The accumulation of hemin causes destruction of red blood cells (RBC) membranes. In this study the process of development of topological defects of RBC membranes within the size range from nanoscale to microscale levels is shown. The formation of the grain-like structures in the membrane ("grains") with typical sizes of 120-200 nm was experimentally shown. The process of formation of "grains" was dependent on the hemin concentration and incubation time. The possible mechanism of membrane nanostructure alterations is proposed. The kinetic equations of formation and transformation of small and medium topological defects were analyzed. This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.

Show MeSH

Related in: MedlinePlus

Profiles and histograms of spatial periods of membrane nanostructures for the control cells and cells after the hemin action.(a) Profile of the control membrane. (b) Profile of the membrane surface in the domain. (s) Histogram of the spatial periods of structures for the control membrane. (d) Histogram of the spatial periods in the field of domains. Interaction time of hemin with RBCs was 1 hour, C = 1.5 mM. Profiles are represented as typical for given concentration and incubation time among 108 areas. Sample volume for control histograms was 310, after hemin action – 215.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4129419&req=5

f5: Profiles and histograms of spatial periods of membrane nanostructures for the control cells and cells after the hemin action.(a) Profile of the control membrane. (b) Profile of the membrane surface in the domain. (s) Histogram of the spatial periods of structures for the control membrane. (d) Histogram of the spatial periods in the field of domains. Interaction time of hemin with RBCs was 1 hour, C = 1.5 mM. Profiles are represented as typical for given concentration and incubation time among 108 areas. Sample volume for control histograms was 310, after hemin action – 215.

Mentions: The formation of cells with “grains”-containing domains was highly reproducible. Fig. 4 demonstrates five cells each containing “grains” scanned by AFM. The examples of structures of separate domains and a separate “grain” structure are shown in Fig. 4b (AFM 2D-image) and in Fig. 4c (AFM 3D-image) respectively. The profiles of nanostructures of cell surface patterns in the control and hemin-treated membranes were different (Fig. 5a and Fig. 5b). The histogram of typical space periods L in the profile of the control membrane was bimodal (Fig. 5c) including two peaks L1max = 80–90 nm and L2max = 140–150 nm. After the hemin action the typical space period was unimodal with the peak 160–175 nm (Fig. 5d). The proposed mechanism of this phenomenon will be described in the Discussion section.


Transformation of membrane nanosurface of red blood cells under hemin action.

Kozlova E, Chernysh A, Moroz V, Gudkova O, Sergunova V, Kuzovlev A - Sci Rep (2014)

Profiles and histograms of spatial periods of membrane nanostructures for the control cells and cells after the hemin action.(a) Profile of the control membrane. (b) Profile of the membrane surface in the domain. (s) Histogram of the spatial periods of structures for the control membrane. (d) Histogram of the spatial periods in the field of domains. Interaction time of hemin with RBCs was 1 hour, C = 1.5 mM. Profiles are represented as typical for given concentration and incubation time among 108 areas. Sample volume for control histograms was 310, after hemin action – 215.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4129419&req=5

f5: Profiles and histograms of spatial periods of membrane nanostructures for the control cells and cells after the hemin action.(a) Profile of the control membrane. (b) Profile of the membrane surface in the domain. (s) Histogram of the spatial periods of structures for the control membrane. (d) Histogram of the spatial periods in the field of domains. Interaction time of hemin with RBCs was 1 hour, C = 1.5 mM. Profiles are represented as typical for given concentration and incubation time among 108 areas. Sample volume for control histograms was 310, after hemin action – 215.
Mentions: The formation of cells with “grains”-containing domains was highly reproducible. Fig. 4 demonstrates five cells each containing “grains” scanned by AFM. The examples of structures of separate domains and a separate “grain” structure are shown in Fig. 4b (AFM 2D-image) and in Fig. 4c (AFM 3D-image) respectively. The profiles of nanostructures of cell surface patterns in the control and hemin-treated membranes were different (Fig. 5a and Fig. 5b). The histogram of typical space periods L in the profile of the control membrane was bimodal (Fig. 5c) including two peaks L1max = 80–90 nm and L2max = 140–150 nm. After the hemin action the typical space period was unimodal with the peak 160–175 nm (Fig. 5d). The proposed mechanism of this phenomenon will be described in the Discussion section.

Bottom Line: The process of formation of "grains" was dependent on the hemin concentration and incubation time.The possible mechanism of membrane nanostructure alterations is proposed.This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.

View Article: PubMed Central - PubMed

Affiliation: V.A. Negovsky Scientific Research Institute of General Reanimatology RAS, Moscow, Russian Federation.

ABSTRACT
Hemin is the product of hemoglobin oxidation. Some diseases may lead to a formation of hemin. The accumulation of hemin causes destruction of red blood cells (RBC) membranes. In this study the process of development of topological defects of RBC membranes within the size range from nanoscale to microscale levels is shown. The formation of the grain-like structures in the membrane ("grains") with typical sizes of 120-200 nm was experimentally shown. The process of formation of "grains" was dependent on the hemin concentration and incubation time. The possible mechanism of membrane nanostructure alterations is proposed. The kinetic equations of formation and transformation of small and medium topological defects were analyzed. This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.

Show MeSH
Related in: MedlinePlus