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Transformation of membrane nanosurface of red blood cells under hemin action.

Kozlova E, Chernysh A, Moroz V, Gudkova O, Sergunova V, Kuzovlev A - Sci Rep (2014)

Bottom Line: The process of formation of "grains" was dependent on the hemin concentration and incubation time.The possible mechanism of membrane nanostructure alterations is proposed.This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.

View Article: PubMed Central - PubMed

Affiliation: V.A. Negovsky Scientific Research Institute of General Reanimatology RAS, Moscow, Russian Federation.

ABSTRACT
Hemin is the product of hemoglobin oxidation. Some diseases may lead to a formation of hemin. The accumulation of hemin causes destruction of red blood cells (RBC) membranes. In this study the process of development of topological defects of RBC membranes within the size range from nanoscale to microscale levels is shown. The formation of the grain-like structures in the membrane ("grains") with typical sizes of 120-200 nm was experimentally shown. The process of formation of "grains" was dependent on the hemin concentration and incubation time. The possible mechanism of membrane nanostructure alterations is proposed. The kinetic equations of formation and transformation of small and medium topological defects were analyzed. This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.

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Kinetics of formation and development of topologic defects.(a) Theoretical curves of dependences of the number of small defects (“grains”) on time n(t) and of the number of medium defects (merged “grains”) on time N (t). (b–d) - AFM 2D- image of membrane surface: small – separate “grains” in domains (b), medium – merged “grains” in domains (c), large – merging of domains. (d). Values t, n(t) and N(t) are in relative units.
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f10: Kinetics of formation and development of topologic defects.(a) Theoretical curves of dependences of the number of small defects (“grains”) on time n(t) and of the number of medium defects (merged “grains”) on time N (t). (b–d) - AFM 2D- image of membrane surface: small – separate “grains” in domains (b), medium – merged “grains” in domains (c), large – merging of domains. (d). Values t, n(t) and N(t) are in relative units.

Mentions: In our experiments we detected various types of topologic defects on the membrane (Fig. 10b,c,d). They can be divided into 3 classes. Small – separate “grains” (Fig. 8a, Fig. 10b) have typical spatial period 120–200 nm. Medium – merged 2–5 “grains” (Fig. 9a and Fig. 10c), have spatial period 300–500 nm. Large – the result of merging of medium defects (Fig. 10d) have the size of 500–1500 nm. Let us discuss the kinetics of defects formation and transformation.


Transformation of membrane nanosurface of red blood cells under hemin action.

Kozlova E, Chernysh A, Moroz V, Gudkova O, Sergunova V, Kuzovlev A - Sci Rep (2014)

Kinetics of formation and development of topologic defects.(a) Theoretical curves of dependences of the number of small defects (“grains”) on time n(t) and of the number of medium defects (merged “grains”) on time N (t). (b–d) - AFM 2D- image of membrane surface: small – separate “grains” in domains (b), medium – merged “grains” in domains (c), large – merging of domains. (d). Values t, n(t) and N(t) are in relative units.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4129419&req=5

f10: Kinetics of formation and development of topologic defects.(a) Theoretical curves of dependences of the number of small defects (“grains”) on time n(t) and of the number of medium defects (merged “grains”) on time N (t). (b–d) - AFM 2D- image of membrane surface: small – separate “grains” in domains (b), medium – merged “grains” in domains (c), large – merging of domains. (d). Values t, n(t) and N(t) are in relative units.
Mentions: In our experiments we detected various types of topologic defects on the membrane (Fig. 10b,c,d). They can be divided into 3 classes. Small – separate “grains” (Fig. 8a, Fig. 10b) have typical spatial period 120–200 nm. Medium – merged 2–5 “grains” (Fig. 9a and Fig. 10c), have spatial period 300–500 nm. Large – the result of merging of medium defects (Fig. 10d) have the size of 500–1500 nm. Let us discuss the kinetics of defects formation and transformation.

Bottom Line: The process of formation of "grains" was dependent on the hemin concentration and incubation time.The possible mechanism of membrane nanostructure alterations is proposed.This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.

View Article: PubMed Central - PubMed

Affiliation: V.A. Negovsky Scientific Research Institute of General Reanimatology RAS, Moscow, Russian Federation.

ABSTRACT
Hemin is the product of hemoglobin oxidation. Some diseases may lead to a formation of hemin. The accumulation of hemin causes destruction of red blood cells (RBC) membranes. In this study the process of development of topological defects of RBC membranes within the size range from nanoscale to microscale levels is shown. The formation of the grain-like structures in the membrane ("grains") with typical sizes of 120-200 nm was experimentally shown. The process of formation of "grains" was dependent on the hemin concentration and incubation time. The possible mechanism of membrane nanostructure alterations is proposed. The kinetic equations of formation and transformation of small and medium topological defects were analyzed. This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.

Show MeSH
Related in: MedlinePlus