Limits...
Phylogenetic evidence for multiple intertypic recombinations in enterovirus B81 strains isolated in Tibet, China.

Hu L, Zhang Y, Hong M, Zhu S, Yan D, Wang D, Li X, Zhu Z - Sci Rep (2014)

Bottom Line: The sequence comparison and phylogenetic dendrogram analysis revealed high variability among the global EV-B81 strains and frequent intertypic recombination in the non-structural protein region of EV-B serotypes, suggesting high genetic diversity of EV-B81.Hence, the extent of transmission and the exposure of the population to this EV type are very limited.Although little is known about the biological and pathogenic properties of EV-B81 because of few research in this field owing to the limited number of isolates, our study provides basic information for further studies of EV-B81.

View Article: PubMed Central - PubMed

Affiliation: WHO WPRO Regional Polio Reference Laboratory and Ministry of Health Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.

ABSTRACT
Enterovirus B81 (EV-B81) is a newly identified serotype within the species enterovirus B (EV-B). To date, only eight nucleotide sequences of EV-B81 have been published and only one full-length genome sequence (the prototype strain) has been made available in the GenBank database. Here, we report the full-length genome sequences of two EV-B81 strains isolated in the Tibet Autonomous Region of China during acute flaccid paralysis surveillance activities, and we also conducted an antibody seroprevalence study in two prefectures of Tibet. The sequence comparison and phylogenetic dendrogram analysis revealed high variability among the global EV-B81 strains and frequent intertypic recombination in the non-structural protein region of EV-B serotypes, suggesting high genetic diversity of EV-B81. However, low positive rates and low titers of neutralizing antibodies against EV-B81 were detected. Nearly 68% of children under the age of five had no neutralizing antibodies against EV-B81. Hence, the extent of transmission and the exposure of the population to this EV type are very limited. Although little is known about the biological and pathogenic properties of EV-B81 because of few research in this field owing to the limited number of isolates, our study provides basic information for further studies of EV-B81.

Show MeSH

Related in: MedlinePlus

Recombination analyses of complete enterovirus B (EV-B) genomes.(a) Similarity plot and (b) bootscanning analysis. A sliding window of 200 nucleotides was used, moving in 20-nucleotide steps. The Tibetan EV-B81 strain 99279/XZ/CHN/1999 was used as a query sequence (indicated in the upper right corner of the image).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4129410&req=5

f3: Recombination analyses of complete enterovirus B (EV-B) genomes.(a) Similarity plot and (b) bootscanning analysis. A sliding window of 200 nucleotides was used, moving in 20-nucleotide steps. The Tibetan EV-B81 strain 99279/XZ/CHN/1999 was used as a query sequence (indicated in the upper right corner of the image).

Mentions: Similarity plots and bootscanning analyses were performed to confirm the recombinations between the Tibetan EV-B81 strains and other EV-B prototype strains. Because the two Tibetan EV-B81 strains share high nucleotide identity in their full-length genomes (99.6%), only one of the strains (99279) was used as a query sequence. It was compared with the EV-B81 prototype strain (USA/CA68-10389) and other EV-B prototype strains. In the P1 coding region, strain 99279 possessed the highest similarity with the EV-B81 prototype strain as expected (Fig. 3). However, in the 5′ UTR, P2, P3, and 3′ UTR regions, strain 99279 was apparently not related to the EV-B81 prototype strain, which further confirmed the occurrence of recombination in these regions. Interestingly, a relatively high similarity within the 3′ end of the 2C region to the 5′ end of the 3C region was found between strain 99279 and the EV-B111 prototype strain, which was supported by the bootscanning analysis. And the bootscanning analysis also suggested the possibility of recombination of small-sized fragment in 5′ UTR, 3D, and 3′ UTR region between Chinese EV-B81 strains and other EV-B serotypes such as EV-B86, EV-B107, and EV-B87. (Fig. 3).


Phylogenetic evidence for multiple intertypic recombinations in enterovirus B81 strains isolated in Tibet, China.

Hu L, Zhang Y, Hong M, Zhu S, Yan D, Wang D, Li X, Zhu Z - Sci Rep (2014)

Recombination analyses of complete enterovirus B (EV-B) genomes.(a) Similarity plot and (b) bootscanning analysis. A sliding window of 200 nucleotides was used, moving in 20-nucleotide steps. The Tibetan EV-B81 strain 99279/XZ/CHN/1999 was used as a query sequence (indicated in the upper right corner of the image).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4129410&req=5

f3: Recombination analyses of complete enterovirus B (EV-B) genomes.(a) Similarity plot and (b) bootscanning analysis. A sliding window of 200 nucleotides was used, moving in 20-nucleotide steps. The Tibetan EV-B81 strain 99279/XZ/CHN/1999 was used as a query sequence (indicated in the upper right corner of the image).
Mentions: Similarity plots and bootscanning analyses were performed to confirm the recombinations between the Tibetan EV-B81 strains and other EV-B prototype strains. Because the two Tibetan EV-B81 strains share high nucleotide identity in their full-length genomes (99.6%), only one of the strains (99279) was used as a query sequence. It was compared with the EV-B81 prototype strain (USA/CA68-10389) and other EV-B prototype strains. In the P1 coding region, strain 99279 possessed the highest similarity with the EV-B81 prototype strain as expected (Fig. 3). However, in the 5′ UTR, P2, P3, and 3′ UTR regions, strain 99279 was apparently not related to the EV-B81 prototype strain, which further confirmed the occurrence of recombination in these regions. Interestingly, a relatively high similarity within the 3′ end of the 2C region to the 5′ end of the 3C region was found between strain 99279 and the EV-B111 prototype strain, which was supported by the bootscanning analysis. And the bootscanning analysis also suggested the possibility of recombination of small-sized fragment in 5′ UTR, 3D, and 3′ UTR region between Chinese EV-B81 strains and other EV-B serotypes such as EV-B86, EV-B107, and EV-B87. (Fig. 3).

Bottom Line: The sequence comparison and phylogenetic dendrogram analysis revealed high variability among the global EV-B81 strains and frequent intertypic recombination in the non-structural protein region of EV-B serotypes, suggesting high genetic diversity of EV-B81.Hence, the extent of transmission and the exposure of the population to this EV type are very limited.Although little is known about the biological and pathogenic properties of EV-B81 because of few research in this field owing to the limited number of isolates, our study provides basic information for further studies of EV-B81.

View Article: PubMed Central - PubMed

Affiliation: WHO WPRO Regional Polio Reference Laboratory and Ministry of Health Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.

ABSTRACT
Enterovirus B81 (EV-B81) is a newly identified serotype within the species enterovirus B (EV-B). To date, only eight nucleotide sequences of EV-B81 have been published and only one full-length genome sequence (the prototype strain) has been made available in the GenBank database. Here, we report the full-length genome sequences of two EV-B81 strains isolated in the Tibet Autonomous Region of China during acute flaccid paralysis surveillance activities, and we also conducted an antibody seroprevalence study in two prefectures of Tibet. The sequence comparison and phylogenetic dendrogram analysis revealed high variability among the global EV-B81 strains and frequent intertypic recombination in the non-structural protein region of EV-B serotypes, suggesting high genetic diversity of EV-B81. However, low positive rates and low titers of neutralizing antibodies against EV-B81 were detected. Nearly 68% of children under the age of five had no neutralizing antibodies against EV-B81. Hence, the extent of transmission and the exposure of the population to this EV type are very limited. Although little is known about the biological and pathogenic properties of EV-B81 because of few research in this field owing to the limited number of isolates, our study provides basic information for further studies of EV-B81.

Show MeSH
Related in: MedlinePlus