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Phylogenetic evidence for multiple intertypic recombinations in enterovirus B81 strains isolated in Tibet, China.

Hu L, Zhang Y, Hong M, Zhu S, Yan D, Wang D, Li X, Zhu Z - Sci Rep (2014)

Bottom Line: The sequence comparison and phylogenetic dendrogram analysis revealed high variability among the global EV-B81 strains and frequent intertypic recombination in the non-structural protein region of EV-B serotypes, suggesting high genetic diversity of EV-B81.Hence, the extent of transmission and the exposure of the population to this EV type are very limited.Although little is known about the biological and pathogenic properties of EV-B81 because of few research in this field owing to the limited number of isolates, our study provides basic information for further studies of EV-B81.

View Article: PubMed Central - PubMed

Affiliation: WHO WPRO Regional Polio Reference Laboratory and Ministry of Health Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.

ABSTRACT
Enterovirus B81 (EV-B81) is a newly identified serotype within the species enterovirus B (EV-B). To date, only eight nucleotide sequences of EV-B81 have been published and only one full-length genome sequence (the prototype strain) has been made available in the GenBank database. Here, we report the full-length genome sequences of two EV-B81 strains isolated in the Tibet Autonomous Region of China during acute flaccid paralysis surveillance activities, and we also conducted an antibody seroprevalence study in two prefectures of Tibet. The sequence comparison and phylogenetic dendrogram analysis revealed high variability among the global EV-B81 strains and frequent intertypic recombination in the non-structural protein region of EV-B serotypes, suggesting high genetic diversity of EV-B81. However, low positive rates and low titers of neutralizing antibodies against EV-B81 were detected. Nearly 68% of children under the age of five had no neutralizing antibodies against EV-B81. Hence, the extent of transmission and the exposure of the population to this EV type are very limited. Although little is known about the biological and pathogenic properties of EV-B81 because of few research in this field owing to the limited number of isolates, our study provides basic information for further studies of EV-B81.

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Phylogenetic relationships based on the VP1, P1, P2, and P3 genome regions of enterovirus B (EV-B).Two Tibetan EV-B81 strains (indicated by solid circles) and 55 other EV-B prototype strains were analyzed by nucleotide sequence alignment using the Neighbor-Joining algorithms implemented in the MEGA 5.0 program. Numbers at the nodes indicate bootstrap support for that node (percent of 1000 bootstrap replicates). The open triangle indicates the India EV-B81 which has the entire VP1 sequence in the GenBank database, and the solid diamond indicates EV-B81 prototype strain. The scale bars represent the genetic distance. All panels have the same scale. (a) VP1 coding sequences; (b) P1 coding sequences; (c) P2 coding sequences; and (d) P3 coding sequences.
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f2: Phylogenetic relationships based on the VP1, P1, P2, and P3 genome regions of enterovirus B (EV-B).Two Tibetan EV-B81 strains (indicated by solid circles) and 55 other EV-B prototype strains were analyzed by nucleotide sequence alignment using the Neighbor-Joining algorithms implemented in the MEGA 5.0 program. Numbers at the nodes indicate bootstrap support for that node (percent of 1000 bootstrap replicates). The open triangle indicates the India EV-B81 which has the entire VP1 sequence in the GenBank database, and the solid diamond indicates EV-B81 prototype strain. The scale bars represent the genetic distance. All panels have the same scale. (a) VP1 coding sequences; (b) P1 coding sequences; (c) P2 coding sequences; and (d) P3 coding sequences.

Mentions: To investigate the genetic relationship between the Tibetan EV-B81 strains, the EV-B81 prototype strain, and other EV-B prototype strains available in the GenBank database, we constructed phylogenetic trees based on the VP1, P1, P2, and P3 regions of the genome. The phylogenetic tree based on the VP1 region also contained an EV-B81 strain (N-428/IND/2008) isolated from India, for which the entire VP1 sequence was available in the GenBank database (Fig. 2). In the VP1 and P1 capsid regions, the two Tibetan EV-B81 strains clustered together with the EV-B81 prototype strain and the Indian EV-B81 strain, confirming the preliminary molecular typing results. In the VP1 region, the nucleotide identity between the Tibetan strains 99279 and 99298c and the Indian strain was 88.8% and 89.1%, respectively. However, in the non-capsid regions, the phylogenetic trees differed greatly from those in the capsid regions. In the P2 and P3 regions, the two Tibetan EV-B81 strains shared the highest similarity with the prototype strains of EV-B111 and EV-B86, respectively. This surprised us because EV-B111 is a recently reported type of enterovirus15. The phylogenetic analysis indicated that recombinations between Chinese EV-B81 strains and other EV-B serotypes might have occurred.


Phylogenetic evidence for multiple intertypic recombinations in enterovirus B81 strains isolated in Tibet, China.

Hu L, Zhang Y, Hong M, Zhu S, Yan D, Wang D, Li X, Zhu Z - Sci Rep (2014)

Phylogenetic relationships based on the VP1, P1, P2, and P3 genome regions of enterovirus B (EV-B).Two Tibetan EV-B81 strains (indicated by solid circles) and 55 other EV-B prototype strains were analyzed by nucleotide sequence alignment using the Neighbor-Joining algorithms implemented in the MEGA 5.0 program. Numbers at the nodes indicate bootstrap support for that node (percent of 1000 bootstrap replicates). The open triangle indicates the India EV-B81 which has the entire VP1 sequence in the GenBank database, and the solid diamond indicates EV-B81 prototype strain. The scale bars represent the genetic distance. All panels have the same scale. (a) VP1 coding sequences; (b) P1 coding sequences; (c) P2 coding sequences; and (d) P3 coding sequences.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4129410&req=5

f2: Phylogenetic relationships based on the VP1, P1, P2, and P3 genome regions of enterovirus B (EV-B).Two Tibetan EV-B81 strains (indicated by solid circles) and 55 other EV-B prototype strains were analyzed by nucleotide sequence alignment using the Neighbor-Joining algorithms implemented in the MEGA 5.0 program. Numbers at the nodes indicate bootstrap support for that node (percent of 1000 bootstrap replicates). The open triangle indicates the India EV-B81 which has the entire VP1 sequence in the GenBank database, and the solid diamond indicates EV-B81 prototype strain. The scale bars represent the genetic distance. All panels have the same scale. (a) VP1 coding sequences; (b) P1 coding sequences; (c) P2 coding sequences; and (d) P3 coding sequences.
Mentions: To investigate the genetic relationship between the Tibetan EV-B81 strains, the EV-B81 prototype strain, and other EV-B prototype strains available in the GenBank database, we constructed phylogenetic trees based on the VP1, P1, P2, and P3 regions of the genome. The phylogenetic tree based on the VP1 region also contained an EV-B81 strain (N-428/IND/2008) isolated from India, for which the entire VP1 sequence was available in the GenBank database (Fig. 2). In the VP1 and P1 capsid regions, the two Tibetan EV-B81 strains clustered together with the EV-B81 prototype strain and the Indian EV-B81 strain, confirming the preliminary molecular typing results. In the VP1 region, the nucleotide identity between the Tibetan strains 99279 and 99298c and the Indian strain was 88.8% and 89.1%, respectively. However, in the non-capsid regions, the phylogenetic trees differed greatly from those in the capsid regions. In the P2 and P3 regions, the two Tibetan EV-B81 strains shared the highest similarity with the prototype strains of EV-B111 and EV-B86, respectively. This surprised us because EV-B111 is a recently reported type of enterovirus15. The phylogenetic analysis indicated that recombinations between Chinese EV-B81 strains and other EV-B serotypes might have occurred.

Bottom Line: The sequence comparison and phylogenetic dendrogram analysis revealed high variability among the global EV-B81 strains and frequent intertypic recombination in the non-structural protein region of EV-B serotypes, suggesting high genetic diversity of EV-B81.Hence, the extent of transmission and the exposure of the population to this EV type are very limited.Although little is known about the biological and pathogenic properties of EV-B81 because of few research in this field owing to the limited number of isolates, our study provides basic information for further studies of EV-B81.

View Article: PubMed Central - PubMed

Affiliation: WHO WPRO Regional Polio Reference Laboratory and Ministry of Health Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.

ABSTRACT
Enterovirus B81 (EV-B81) is a newly identified serotype within the species enterovirus B (EV-B). To date, only eight nucleotide sequences of EV-B81 have been published and only one full-length genome sequence (the prototype strain) has been made available in the GenBank database. Here, we report the full-length genome sequences of two EV-B81 strains isolated in the Tibet Autonomous Region of China during acute flaccid paralysis surveillance activities, and we also conducted an antibody seroprevalence study in two prefectures of Tibet. The sequence comparison and phylogenetic dendrogram analysis revealed high variability among the global EV-B81 strains and frequent intertypic recombination in the non-structural protein region of EV-B serotypes, suggesting high genetic diversity of EV-B81. However, low positive rates and low titers of neutralizing antibodies against EV-B81 were detected. Nearly 68% of children under the age of five had no neutralizing antibodies against EV-B81. Hence, the extent of transmission and the exposure of the population to this EV type are very limited. Although little is known about the biological and pathogenic properties of EV-B81 because of few research in this field owing to the limited number of isolates, our study provides basic information for further studies of EV-B81.

Show MeSH
Related in: MedlinePlus