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Characteristic of c-Kit+ progenitor cells in explanted human hearts.

Matuszczak S, Czapla J, Jarosz-Biej M, Wiśniewska E, Cichoń T, Smolarczyk R, Kobusińska M, Gajda K, Wilczek P, Sliwka J, Zembala M, Zembala M, Szala S - Clin Res Cardiol (2014)

Bottom Line: In contrast to published data we do not observed statistically significant differences in the number of c-Kit(+) cells between disease-specific groups, parts of the heart or sexes.Nevertheless, c-Kit(+) cells were present in significant numbers (11-24%) in samples derived from three explanted pediatric hearts. c-Kit(+) cells were also positive for CD105 and a majority of them was positive for CD31 and CD34 (83.7 ± 8.6 and 75.7 ± 11.4%, respectively).Isolated c-Kit(+) cells display mesenchymal stem cell features and are thought to differentiate into endothelial cells.

View Article: PubMed Central - PubMed

Affiliation: Center for Translational Research and Molecular Biology of Cancer, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeże Armii Krajowej Street 15, 44-101, Gliwice, Poland.

ABSTRACT
According to literature data, self-renewing, multipotent, and clonogenic cardiac c-Kit(+) progenitor cells occur within human myocardium. The aim of this study was to isolate and characterize c-Kit(+) progenitor cells from explanted human hearts. Experimental material was obtained from 19 adult and 7 pediatric patients. Successful isolation and culture was achieved for 95 samples (84.1%) derived from five different regions of the heart: right and left ventricles, atrium, intraventricular septum, and apex. The average percentage of c-Kit(+) cells, as assessed by FACS, ranged between 0.7 and 0.9%. In contrast to published data we do not observed statistically significant differences in the number of c-Kit(+) cells between disease-specific groups, parts of the heart or sexes. Nevertheless, c-Kit(+) cells were present in significant numbers (11-24%) in samples derived from three explanted pediatric hearts. c-Kit(+) cells were also positive for CD105 and a majority of them was positive for CD31 and CD34 (83.7 ± 8.6 and 75.7 ± 11.4%, respectively). Immunohistochemical analysis of the heart tissue revealed that most cells possessing the c-Kit antigen were also positive for tryptase, a specific mast cell marker. However, flow cytometry analysis has shown cultured c-Kit(+) cells to be negative for hematopoietic marker CD45 and mast cell marker CD33. Isolated c-Kit(+) cells display mesenchymal stem cell features and are thought to differentiate into endothelial cells.

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Identification of c-Kit+ cells in human heart. a The arrow indicates c-Kit+ (green fluorescence) progenitor cardiac cells, b the arrowhead indicates c-Kit+ (green fluorescence) tryptase+ (white fluorescence) mast cells. A few c-Kit+ tryptase− cells were observed in the human cardiac tissue sections
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Fig2: Identification of c-Kit+ cells in human heart. a The arrow indicates c-Kit+ (green fluorescence) progenitor cardiac cells, b the arrowhead indicates c-Kit+ (green fluorescence) tryptase+ (white fluorescence) mast cells. A few c-Kit+ tryptase− cells were observed in the human cardiac tissue sections

Mentions: A few c-Kit+tryptase− cells were observed in the human cardiac tissue sections (Fig. 2a). Most of the c-Kit+ cells contained tryptase––an enzyme specific to mast cells (Fig. 2b). There were no differences in amount of c-Kit+ tryptase− cells in the material derived from adults and children.Fig. 2


Characteristic of c-Kit+ progenitor cells in explanted human hearts.

Matuszczak S, Czapla J, Jarosz-Biej M, Wiśniewska E, Cichoń T, Smolarczyk R, Kobusińska M, Gajda K, Wilczek P, Sliwka J, Zembala M, Zembala M, Szala S - Clin Res Cardiol (2014)

Identification of c-Kit+ cells in human heart. a The arrow indicates c-Kit+ (green fluorescence) progenitor cardiac cells, b the arrowhead indicates c-Kit+ (green fluorescence) tryptase+ (white fluorescence) mast cells. A few c-Kit+ tryptase− cells were observed in the human cardiac tissue sections
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4129222&req=5

Fig2: Identification of c-Kit+ cells in human heart. a The arrow indicates c-Kit+ (green fluorescence) progenitor cardiac cells, b the arrowhead indicates c-Kit+ (green fluorescence) tryptase+ (white fluorescence) mast cells. A few c-Kit+ tryptase− cells were observed in the human cardiac tissue sections
Mentions: A few c-Kit+tryptase− cells were observed in the human cardiac tissue sections (Fig. 2a). Most of the c-Kit+ cells contained tryptase––an enzyme specific to mast cells (Fig. 2b). There were no differences in amount of c-Kit+ tryptase− cells in the material derived from adults and children.Fig. 2

Bottom Line: In contrast to published data we do not observed statistically significant differences in the number of c-Kit(+) cells between disease-specific groups, parts of the heart or sexes.Nevertheless, c-Kit(+) cells were present in significant numbers (11-24%) in samples derived from three explanted pediatric hearts. c-Kit(+) cells were also positive for CD105 and a majority of them was positive for CD31 and CD34 (83.7 ± 8.6 and 75.7 ± 11.4%, respectively).Isolated c-Kit(+) cells display mesenchymal stem cell features and are thought to differentiate into endothelial cells.

View Article: PubMed Central - PubMed

Affiliation: Center for Translational Research and Molecular Biology of Cancer, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeże Armii Krajowej Street 15, 44-101, Gliwice, Poland.

ABSTRACT
According to literature data, self-renewing, multipotent, and clonogenic cardiac c-Kit(+) progenitor cells occur within human myocardium. The aim of this study was to isolate and characterize c-Kit(+) progenitor cells from explanted human hearts. Experimental material was obtained from 19 adult and 7 pediatric patients. Successful isolation and culture was achieved for 95 samples (84.1%) derived from five different regions of the heart: right and left ventricles, atrium, intraventricular septum, and apex. The average percentage of c-Kit(+) cells, as assessed by FACS, ranged between 0.7 and 0.9%. In contrast to published data we do not observed statistically significant differences in the number of c-Kit(+) cells between disease-specific groups, parts of the heart or sexes. Nevertheless, c-Kit(+) cells were present in significant numbers (11-24%) in samples derived from three explanted pediatric hearts. c-Kit(+) cells were also positive for CD105 and a majority of them was positive for CD31 and CD34 (83.7 ± 8.6 and 75.7 ± 11.4%, respectively). Immunohistochemical analysis of the heart tissue revealed that most cells possessing the c-Kit antigen were also positive for tryptase, a specific mast cell marker. However, flow cytometry analysis has shown cultured c-Kit(+) cells to be negative for hematopoietic marker CD45 and mast cell marker CD33. Isolated c-Kit(+) cells display mesenchymal stem cell features and are thought to differentiate into endothelial cells.

Show MeSH
Related in: MedlinePlus