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HPLC-fingerprints and antioxidant constituents of Phyla nodiflora.

Lin FJ, Yen FL, Chen PC, Wang MC, Lin CN, Lee CW, Ko HH - ScientificWorldJournal (2014)

Bottom Line: In the present study, ten compounds, namely, 3,7,4',5'-tetrahydroxy-3'-methoxyflavone (1), nodifloretin (2), 4'-hydroxywogonin (3), onopordin (4), cirsiliol (5), 5,7,8,4'-tetrahydroxy-3'-methoxyflavone (6), eupafolin (7), hispidulin (8), larycitrin (9), and β-sitosterol were isolated from the methanolic extract of the aerial part of P. nodiflora (PNM) and their structures were identified by 1D-NMR comparing their spectra with the literature.The antioxidant activities of these compounds were evaluated by free radical scavenging activity and tyrosinase inhibitory effect in cell-free systems.Compounds 4, 5, and 7 showed strong antioxidant activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan.

ABSTRACT
Phyla nodiflora is a creeping perennial herb, widely distributed in the most tropical and subtropical regions. It has been used as a folk medicine, herbal beverage, or folk cosmetic. For these usages, the development of a chemical quality control method of this plant is necessary. In the present study, ten compounds, namely, 3,7,4',5'-tetrahydroxy-3'-methoxyflavone (1), nodifloretin (2), 4'-hydroxywogonin (3), onopordin (4), cirsiliol (5), 5,7,8,4'-tetrahydroxy-3'-methoxyflavone (6), eupafolin (7), hispidulin (8), larycitrin (9), and β-sitosterol were isolated from the methanolic extract of the aerial part of P. nodiflora (PNM) and their structures were identified by 1D-NMR comparing their spectra with the literature. The antioxidant activities of these compounds were evaluated by free radical scavenging activity and tyrosinase inhibitory effect in cell-free systems. Compounds 4, 5, and 7 showed strong antioxidant activity. To control the quality of P. nodiflora, a simple and reliable method of high-performance liquid chromatography combined with ultraviolet detector (HPLC-UV) was established for both the fingerprint analysis and the quantitative determination of two selected active compounds, onopordin (4) and eupafolin (7). Statistical analysis of the obtained data demonstrated that our method achieved the desired linearity, precision, and accuracy. The results indicated that the developed method can be used as a quality evaluation method for PNM.

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The HPLC-fingerprints of three extracts of PNM from different manufactures. (A) 2007-02-PNM; (B) 2009-06-PNM; (C) 2010-06-PNM.
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fig4: The HPLC-fingerprints of three extracts of PNM from different manufactures. (A) 2007-02-PNM; (B) 2009-06-PNM; (C) 2010-06-PNM.

Mentions: The established analytical method was applied for the quantitative analysis of two active components, 4 and 7, in three different batches of the aerial part of P. nodiflora collected at the same location in different years and seasons (Figure 4). Each sample was analyzed in triplicate to determine the mean content, and the peaks in chromatograms were identified by comparing the retention times and the online UV spectra with those of the standards. The contents of compounds 4 and 7 were 0.048 and 0.072 mg/g in 2007-02-PNM, 0.296 and 0.474 mg/g in 2009-06-PNM, and 0.545 and 0.381 mg/g in 2010-06-PNM, respectively. The quantitative analysis results showed that the crude extract of PNM, even in the different seasons (spring and summer), generally contained the two selected active constituents. From the results obtained, the contents of active compounds were higher in the summer than in the spring. It is known that the different growing place, climate, and harvest processing may affect the level of these compounds. Although PNM in the autumn and in the winter was not collected and detected, the results showed PNM in blossom season (May to August, summer), yielding more active compounds, might serve the good quality and quantity of PNM when used as a cosmetic or herbal medicine additive.


HPLC-fingerprints and antioxidant constituents of Phyla nodiflora.

Lin FJ, Yen FL, Chen PC, Wang MC, Lin CN, Lee CW, Ko HH - ScientificWorldJournal (2014)

The HPLC-fingerprints of three extracts of PNM from different manufactures. (A) 2007-02-PNM; (B) 2009-06-PNM; (C) 2010-06-PNM.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4129175&req=5

fig4: The HPLC-fingerprints of three extracts of PNM from different manufactures. (A) 2007-02-PNM; (B) 2009-06-PNM; (C) 2010-06-PNM.
Mentions: The established analytical method was applied for the quantitative analysis of two active components, 4 and 7, in three different batches of the aerial part of P. nodiflora collected at the same location in different years and seasons (Figure 4). Each sample was analyzed in triplicate to determine the mean content, and the peaks in chromatograms were identified by comparing the retention times and the online UV spectra with those of the standards. The contents of compounds 4 and 7 were 0.048 and 0.072 mg/g in 2007-02-PNM, 0.296 and 0.474 mg/g in 2009-06-PNM, and 0.545 and 0.381 mg/g in 2010-06-PNM, respectively. The quantitative analysis results showed that the crude extract of PNM, even in the different seasons (spring and summer), generally contained the two selected active constituents. From the results obtained, the contents of active compounds were higher in the summer than in the spring. It is known that the different growing place, climate, and harvest processing may affect the level of these compounds. Although PNM in the autumn and in the winter was not collected and detected, the results showed PNM in blossom season (May to August, summer), yielding more active compounds, might serve the good quality and quantity of PNM when used as a cosmetic or herbal medicine additive.

Bottom Line: In the present study, ten compounds, namely, 3,7,4',5'-tetrahydroxy-3'-methoxyflavone (1), nodifloretin (2), 4'-hydroxywogonin (3), onopordin (4), cirsiliol (5), 5,7,8,4'-tetrahydroxy-3'-methoxyflavone (6), eupafolin (7), hispidulin (8), larycitrin (9), and β-sitosterol were isolated from the methanolic extract of the aerial part of P. nodiflora (PNM) and their structures were identified by 1D-NMR comparing their spectra with the literature.The antioxidant activities of these compounds were evaluated by free radical scavenging activity and tyrosinase inhibitory effect in cell-free systems.Compounds 4, 5, and 7 showed strong antioxidant activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan.

ABSTRACT
Phyla nodiflora is a creeping perennial herb, widely distributed in the most tropical and subtropical regions. It has been used as a folk medicine, herbal beverage, or folk cosmetic. For these usages, the development of a chemical quality control method of this plant is necessary. In the present study, ten compounds, namely, 3,7,4',5'-tetrahydroxy-3'-methoxyflavone (1), nodifloretin (2), 4'-hydroxywogonin (3), onopordin (4), cirsiliol (5), 5,7,8,4'-tetrahydroxy-3'-methoxyflavone (6), eupafolin (7), hispidulin (8), larycitrin (9), and β-sitosterol were isolated from the methanolic extract of the aerial part of P. nodiflora (PNM) and their structures were identified by 1D-NMR comparing their spectra with the literature. The antioxidant activities of these compounds were evaluated by free radical scavenging activity and tyrosinase inhibitory effect in cell-free systems. Compounds 4, 5, and 7 showed strong antioxidant activity. To control the quality of P. nodiflora, a simple and reliable method of high-performance liquid chromatography combined with ultraviolet detector (HPLC-UV) was established for both the fingerprint analysis and the quantitative determination of two selected active compounds, onopordin (4) and eupafolin (7). Statistical analysis of the obtained data demonstrated that our method achieved the desired linearity, precision, and accuracy. The results indicated that the developed method can be used as a quality evaluation method for PNM.

Show MeSH