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IL-17 genetic and immunophenotypic evaluation in chronic graft-versus-host disease.

Resende RG, Correia-Silva Jde F, Silva TA, Salomão UE, Marques-Silva L, Vieira ÉL, Dutra WO, Gomez RS - Mediators Inflamm. (2014)

Bottom Line: Although interleukin-17 (IL-17) is a recently discovered cytokine associated with several autoimmune diseases, its role in the pathogenesis of chronic graft-versus-host disease (cGVHD) was not established yet.Lower IL-17A levels in the blood were associated with AA genotype.In flow cytometry analysis, decreased expression of IL-17A was observed in patients with cGVHD after stimulation.

View Article: PubMed Central - PubMed

Affiliation: Serviço de Estomatologia, Hospital Municipal Odilon Behrens, Rua Formiga 50, 31110-430 Belo Horizonte, MG, Brazil.

ABSTRACT
Although interleukin-17 (IL-17) is a recently discovered cytokine associated with several autoimmune diseases, its role in the pathogenesis of chronic graft-versus-host disease (cGVHD) was not established yet. The objective of this study was to investigate the association of IL17A and IL17F genes polymorphisms and IL-17A and IL-17F levels with cGVHD. IL-17A expression was also investigated in CD4(+) T cells of patients with systemic cGVHD. For Part I of the study, fifty-eight allo-HSCT recipients and donors were prospectively studied. Blood samples were obtained to determine IL17A and IL17F genes polymorphisms. Cytokines levels in blood and saliva were assessed by ELISA at days +35 and +100 after HSCT. In Part II, for the immunophenotypic evaluation, eight patients with systemic cGVHD were selected and the expression of IL-17A was evaluated. We found association between recipient AA genotype with systemic cGVHD. No association was observed between IL-17A levels and cGVHD. Lower IL-17A levels in the blood were associated with AA genotype. In flow cytometry analysis, decreased expression of IL-17A was observed in patients with cGVHD after stimulation. In conclusion, IL-17A may have an important role in the development of systemic cGVHD.

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Related in: MedlinePlus

IL-17A expression by CD4+ T cells from HSCT patients with cGVHD following culture with SEB and αCD3αCD28. Whole blood from HSCT patients with cGVHD was maintained in culture without stimulus (media), as well as with SEB and αCD3αCD28. IL-17A (a) expression in CD4+ T cells. The * indicates a P value <0.05 between media and stimulus conditions using Wilcoxon's matched pairs test. The overlap histogram graphics represent IL-17 (b) expression in CD4+ T cells.
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fig2: IL-17A expression by CD4+ T cells from HSCT patients with cGVHD following culture with SEB and αCD3αCD28. Whole blood from HSCT patients with cGVHD was maintained in culture without stimulus (media), as well as with SEB and αCD3αCD28. IL-17A (a) expression in CD4+ T cells. The * indicates a P value <0.05 between media and stimulus conditions using Wilcoxon's matched pairs test. The overlap histogram graphics represent IL-17 (b) expression in CD4+ T cells.

Mentions: Decreased IL-17A expression was observed in total CD4 T cells of patients with cGVHD after stimulation with SEB (P = 0.008) and αCD3αCD28 (P = 0.008) stimulus, compared to media-stimulated control cells (Figure 2(a)). In patients without cGVHD or in healthy individuals, the expression of IL-17A in total CD4 T cells after stimulation with SEB or αCD3αCD2 was not statistically different than that seen in media-stimulated control cells (data not shown). Overlapping histograms for IL-17A fluorescence are shown in Figure 2(b).


IL-17 genetic and immunophenotypic evaluation in chronic graft-versus-host disease.

Resende RG, Correia-Silva Jde F, Silva TA, Salomão UE, Marques-Silva L, Vieira ÉL, Dutra WO, Gomez RS - Mediators Inflamm. (2014)

IL-17A expression by CD4+ T cells from HSCT patients with cGVHD following culture with SEB and αCD3αCD28. Whole blood from HSCT patients with cGVHD was maintained in culture without stimulus (media), as well as with SEB and αCD3αCD28. IL-17A (a) expression in CD4+ T cells. The * indicates a P value <0.05 between media and stimulus conditions using Wilcoxon's matched pairs test. The overlap histogram graphics represent IL-17 (b) expression in CD4+ T cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4129170&req=5

fig2: IL-17A expression by CD4+ T cells from HSCT patients with cGVHD following culture with SEB and αCD3αCD28. Whole blood from HSCT patients with cGVHD was maintained in culture without stimulus (media), as well as with SEB and αCD3αCD28. IL-17A (a) expression in CD4+ T cells. The * indicates a P value <0.05 between media and stimulus conditions using Wilcoxon's matched pairs test. The overlap histogram graphics represent IL-17 (b) expression in CD4+ T cells.
Mentions: Decreased IL-17A expression was observed in total CD4 T cells of patients with cGVHD after stimulation with SEB (P = 0.008) and αCD3αCD28 (P = 0.008) stimulus, compared to media-stimulated control cells (Figure 2(a)). In patients without cGVHD or in healthy individuals, the expression of IL-17A in total CD4 T cells after stimulation with SEB or αCD3αCD2 was not statistically different than that seen in media-stimulated control cells (data not shown). Overlapping histograms for IL-17A fluorescence are shown in Figure 2(b).

Bottom Line: Although interleukin-17 (IL-17) is a recently discovered cytokine associated with several autoimmune diseases, its role in the pathogenesis of chronic graft-versus-host disease (cGVHD) was not established yet.Lower IL-17A levels in the blood were associated with AA genotype.In flow cytometry analysis, decreased expression of IL-17A was observed in patients with cGVHD after stimulation.

View Article: PubMed Central - PubMed

Affiliation: Serviço de Estomatologia, Hospital Municipal Odilon Behrens, Rua Formiga 50, 31110-430 Belo Horizonte, MG, Brazil.

ABSTRACT
Although interleukin-17 (IL-17) is a recently discovered cytokine associated with several autoimmune diseases, its role in the pathogenesis of chronic graft-versus-host disease (cGVHD) was not established yet. The objective of this study was to investigate the association of IL17A and IL17F genes polymorphisms and IL-17A and IL-17F levels with cGVHD. IL-17A expression was also investigated in CD4(+) T cells of patients with systemic cGVHD. For Part I of the study, fifty-eight allo-HSCT recipients and donors were prospectively studied. Blood samples were obtained to determine IL17A and IL17F genes polymorphisms. Cytokines levels in blood and saliva were assessed by ELISA at days +35 and +100 after HSCT. In Part II, for the immunophenotypic evaluation, eight patients with systemic cGVHD were selected and the expression of IL-17A was evaluated. We found association between recipient AA genotype with systemic cGVHD. No association was observed between IL-17A levels and cGVHD. Lower IL-17A levels in the blood were associated with AA genotype. In flow cytometry analysis, decreased expression of IL-17A was observed in patients with cGVHD after stimulation. In conclusion, IL-17A may have an important role in the development of systemic cGVHD.

Show MeSH
Related in: MedlinePlus