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Investigation of hepatic blood perfusion by laser speckle imaging and changes of hepatic vasoactive substances in mice after electroacupuncture.

Song XJ, Zhang D, Wang SY, Li SY - Evid Based Complement Alternat Med (2014)

Bottom Line: The study was conducted to observe the effect of electroacupuncture (EA) on hepatic blood perfusion (HBP) and vascular regulation.HBP gradually decreased during 30 min in control group.It is concluded that EA at ST36 could increase HBP possibly by increasing the blood flow velocity (BFV), changing vascular activity, increasing the level of NO, and inhibiting the level of ET-1 in liver tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, 16 Nanxiaojie, Dongzhimen, Beijing 100700, China.

ABSTRACT
The study was conducted to observe the effect of electroacupuncture (EA) on hepatic blood perfusion (HBP) and vascular regulation. We investigated 60 male anesthetized mice under the following 3 conditions: without EA stimulation (control group); EA stimulation at Zusanli (ST36 group); EA stimulation at nonacupoint (NA group) during 30 min. The HBP was measured using the laser speckle perfusion imaging (LSPI). The level of nitric oxide (NO), endothelin-1 (ET-1), and noradrenaline (NE) in liver tissue was detected by biochemical methods. Results were as follows. At each time point, HBP increase in ST36 group was higher than that in the NA group in anesthetized mice. HBP gradually decreased during 30 min in control group. The level of NO in ST36 group was higher than that in NA group. The level of both ET-1 and NE was the highest in control group, followed by NA group and ST36 group. It is concluded that EA at ST36 could increase HBP possibly by increasing the blood flow velocity (BFV), changing vascular activity, increasing the level of NO, and inhibiting the level of ET-1 in liver tissue.

No MeSH data available.


Related in: MedlinePlus

Change of the mean differences of HBP in the three groups. In ST36 or nonpoint group, 0 min was the time before EA; 5–30 min was EA. In control group, 0–30 min was monitoring continually without EA. Data are presented as mean ± SD, n = 20 animals for each group.
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fig4: Change of the mean differences of HBP in the three groups. In ST36 or nonpoint group, 0 min was the time before EA; 5–30 min was EA. In control group, 0–30 min was monitoring continually without EA. Data are presented as mean ± SD, n = 20 animals for each group.

Mentions: HBP in each EA group was significantly increased at every time point as compared with 0 min. After 5 min, the increase of HBP was 295.54 ± 62.77 (PU), increased by 7.65 ± 1.89%. The highest increase was found at 25 min, which was 527.52 ± 75.96 PU, increased by 14.87 ± 2.35% in ST36 group. After 5 min, the increase of HBP was 88.07 ± 33.36 PU, increased by 2.26 ± 0.92%. The highest increase was found at 25 min, which was 154.04 ± 42.33 PU, increased by 4.14 ± 1.1% in NA group. In control group, HBP was decreased slowly during 30 min (Figure 4). It could be seen from Table 1 that at all time points of EA the increasing rate of HBP in NA group and control group was significantly lower than that of ST36 group (P < 0.05).


Investigation of hepatic blood perfusion by laser speckle imaging and changes of hepatic vasoactive substances in mice after electroacupuncture.

Song XJ, Zhang D, Wang SY, Li SY - Evid Based Complement Alternat Med (2014)

Change of the mean differences of HBP in the three groups. In ST36 or nonpoint group, 0 min was the time before EA; 5–30 min was EA. In control group, 0–30 min was monitoring continually without EA. Data are presented as mean ± SD, n = 20 animals for each group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4129169&req=5

fig4: Change of the mean differences of HBP in the three groups. In ST36 or nonpoint group, 0 min was the time before EA; 5–30 min was EA. In control group, 0–30 min was monitoring continually without EA. Data are presented as mean ± SD, n = 20 animals for each group.
Mentions: HBP in each EA group was significantly increased at every time point as compared with 0 min. After 5 min, the increase of HBP was 295.54 ± 62.77 (PU), increased by 7.65 ± 1.89%. The highest increase was found at 25 min, which was 527.52 ± 75.96 PU, increased by 14.87 ± 2.35% in ST36 group. After 5 min, the increase of HBP was 88.07 ± 33.36 PU, increased by 2.26 ± 0.92%. The highest increase was found at 25 min, which was 154.04 ± 42.33 PU, increased by 4.14 ± 1.1% in NA group. In control group, HBP was decreased slowly during 30 min (Figure 4). It could be seen from Table 1 that at all time points of EA the increasing rate of HBP in NA group and control group was significantly lower than that of ST36 group (P < 0.05).

Bottom Line: The study was conducted to observe the effect of electroacupuncture (EA) on hepatic blood perfusion (HBP) and vascular regulation.HBP gradually decreased during 30 min in control group.It is concluded that EA at ST36 could increase HBP possibly by increasing the blood flow velocity (BFV), changing vascular activity, increasing the level of NO, and inhibiting the level of ET-1 in liver tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, 16 Nanxiaojie, Dongzhimen, Beijing 100700, China.

ABSTRACT
The study was conducted to observe the effect of electroacupuncture (EA) on hepatic blood perfusion (HBP) and vascular regulation. We investigated 60 male anesthetized mice under the following 3 conditions: without EA stimulation (control group); EA stimulation at Zusanli (ST36 group); EA stimulation at nonacupoint (NA group) during 30 min. The HBP was measured using the laser speckle perfusion imaging (LSPI). The level of nitric oxide (NO), endothelin-1 (ET-1), and noradrenaline (NE) in liver tissue was detected by biochemical methods. Results were as follows. At each time point, HBP increase in ST36 group was higher than that in the NA group in anesthetized mice. HBP gradually decreased during 30 min in control group. The level of NO in ST36 group was higher than that in NA group. The level of both ET-1 and NE was the highest in control group, followed by NA group and ST36 group. It is concluded that EA at ST36 could increase HBP possibly by increasing the blood flow velocity (BFV), changing vascular activity, increasing the level of NO, and inhibiting the level of ET-1 in liver tissue.

No MeSH data available.


Related in: MedlinePlus