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Investigation of hepatic blood perfusion by laser speckle imaging and changes of hepatic vasoactive substances in mice after electroacupuncture.

Song XJ, Zhang D, Wang SY, Li SY - Evid Based Complement Alternat Med (2014)

Bottom Line: The study was conducted to observe the effect of electroacupuncture (EA) on hepatic blood perfusion (HBP) and vascular regulation.HBP gradually decreased during 30 min in control group.It is concluded that EA at ST36 could increase HBP possibly by increasing the blood flow velocity (BFV), changing vascular activity, increasing the level of NO, and inhibiting the level of ET-1 in liver tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, 16 Nanxiaojie, Dongzhimen, Beijing 100700, China.

ABSTRACT
The study was conducted to observe the effect of electroacupuncture (EA) on hepatic blood perfusion (HBP) and vascular regulation. We investigated 60 male anesthetized mice under the following 3 conditions: without EA stimulation (control group); EA stimulation at Zusanli (ST36 group); EA stimulation at nonacupoint (NA group) during 30 min. The HBP was measured using the laser speckle perfusion imaging (LSPI). The level of nitric oxide (NO), endothelin-1 (ET-1), and noradrenaline (NE) in liver tissue was detected by biochemical methods. Results were as follows. At each time point, HBP increase in ST36 group was higher than that in the NA group in anesthetized mice. HBP gradually decreased during 30 min in control group. The level of NO in ST36 group was higher than that in NA group. The level of both ET-1 and NE was the highest in control group, followed by NA group and ST36 group. It is concluded that EA at ST36 could increase HBP possibly by increasing the blood flow velocity (BFV), changing vascular activity, increasing the level of NO, and inhibiting the level of ET-1 in liver tissue.

No MeSH data available.


Related in: MedlinePlus

Hepatic LSP images in control group without EA. (a) At 0 min; (b) at 5 min; (c) at 10 min; (d) at 15 min; (e) at 20 min; (f) at 25 min; (g) at 30 min; (h) digital coded brightness image of mouse liver. At beginning of observation, LSP image was displayed as light green, yellow, and red on the image. Moreover the red areas gradually reduced and the yellow and greenish distribution gradually increased along with the observation time extended.
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fig3: Hepatic LSP images in control group without EA. (a) At 0 min; (b) at 5 min; (c) at 10 min; (d) at 15 min; (e) at 20 min; (f) at 25 min; (g) at 30 min; (h) digital coded brightness image of mouse liver. At beginning of observation, LSP image was displayed as light green, yellow, and red on the image. Moreover the red areas gradually reduced and the yellow and greenish distribution gradually increased along with the observation time extended.

Mentions: From the LSP image at the starting of investigation (Figure 3(a)), it could be seen that the HBP was rich. The HBP was high in the fan-shaped area close to hepatic portal was displayed dark red on the images, and the HBP was decreased from fan-shaped area to the liver anterior edge region were displayed yellow and light green. As investigation time passed by, the light green and yellow regions were extended, and the red areas were decreased gradually, while HBP reduced slowly (Figure 3).


Investigation of hepatic blood perfusion by laser speckle imaging and changes of hepatic vasoactive substances in mice after electroacupuncture.

Song XJ, Zhang D, Wang SY, Li SY - Evid Based Complement Alternat Med (2014)

Hepatic LSP images in control group without EA. (a) At 0 min; (b) at 5 min; (c) at 10 min; (d) at 15 min; (e) at 20 min; (f) at 25 min; (g) at 30 min; (h) digital coded brightness image of mouse liver. At beginning of observation, LSP image was displayed as light green, yellow, and red on the image. Moreover the red areas gradually reduced and the yellow and greenish distribution gradually increased along with the observation time extended.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4129169&req=5

fig3: Hepatic LSP images in control group without EA. (a) At 0 min; (b) at 5 min; (c) at 10 min; (d) at 15 min; (e) at 20 min; (f) at 25 min; (g) at 30 min; (h) digital coded brightness image of mouse liver. At beginning of observation, LSP image was displayed as light green, yellow, and red on the image. Moreover the red areas gradually reduced and the yellow and greenish distribution gradually increased along with the observation time extended.
Mentions: From the LSP image at the starting of investigation (Figure 3(a)), it could be seen that the HBP was rich. The HBP was high in the fan-shaped area close to hepatic portal was displayed dark red on the images, and the HBP was decreased from fan-shaped area to the liver anterior edge region were displayed yellow and light green. As investigation time passed by, the light green and yellow regions were extended, and the red areas were decreased gradually, while HBP reduced slowly (Figure 3).

Bottom Line: The study was conducted to observe the effect of electroacupuncture (EA) on hepatic blood perfusion (HBP) and vascular regulation.HBP gradually decreased during 30 min in control group.It is concluded that EA at ST36 could increase HBP possibly by increasing the blood flow velocity (BFV), changing vascular activity, increasing the level of NO, and inhibiting the level of ET-1 in liver tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, 16 Nanxiaojie, Dongzhimen, Beijing 100700, China.

ABSTRACT
The study was conducted to observe the effect of electroacupuncture (EA) on hepatic blood perfusion (HBP) and vascular regulation. We investigated 60 male anesthetized mice under the following 3 conditions: without EA stimulation (control group); EA stimulation at Zusanli (ST36 group); EA stimulation at nonacupoint (NA group) during 30 min. The HBP was measured using the laser speckle perfusion imaging (LSPI). The level of nitric oxide (NO), endothelin-1 (ET-1), and noradrenaline (NE) in liver tissue was detected by biochemical methods. Results were as follows. At each time point, HBP increase in ST36 group was higher than that in the NA group in anesthetized mice. HBP gradually decreased during 30 min in control group. The level of NO in ST36 group was higher than that in NA group. The level of both ET-1 and NE was the highest in control group, followed by NA group and ST36 group. It is concluded that EA at ST36 could increase HBP possibly by increasing the blood flow velocity (BFV), changing vascular activity, increasing the level of NO, and inhibiting the level of ET-1 in liver tissue.

No MeSH data available.


Related in: MedlinePlus