Limits...
Computational fluid dynamics modelling of microfluidic channel for dielectrophoretic BioMEMS application.

Low WS, Kadri NA, Abas WA - ScientificWorldJournal (2014)

Bottom Line: We propose a strategy for optimizing distribution of flow in a typical benchtop microfluidic chamber for dielectrophoretic application.It is aimed at encouraging uniform flow velocity along the whole analysis chamber in order to ensure DEP force is evenly applied to biological particle.The design was validated by hydrodynamic flow simulation using COMSOL Multiphysics v4.2a software.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
We propose a strategy for optimizing distribution of flow in a typical benchtop microfluidic chamber for dielectrophoretic application. It is aimed at encouraging uniform flow velocity along the whole analysis chamber in order to ensure DEP force is evenly applied to biological particle. Via the study, we have come up with a constructive strategy in improving the design of microfluidic channel which will greatly facilitate the use of DEP system in laboratory and primarily focus on the relationship between architecture and cell distribution, by resorting to the tubular structure of blood vessels. The design was validated by hydrodynamic flow simulation using COMSOL Multiphysics v4.2a software. Simulations show that the presence of 2-level bifurcation has developed portioning of volumetric flow which produced uniform flow across the channel. However, further bifurcation will reduce the volumetric flow rate, thus causing undesirable deposition of cell suspension around the chamber. Finally, an improvement of microfluidic design with rounded corner is proposed to encourage a uniform cell adhesion within the channel.

Show MeSH

Related in: MedlinePlus

Comparison of average velocity across the width of microfluidic chamber for rounded corner and sharp corner.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4129156&req=5

fig10: Comparison of average velocity across the width of microfluidic chamber for rounded corner and sharp corner.

Mentions: In contrast to previous design in this study, the surface plot indicates a uniform velocity profile throughout the channel in the first bifurcation, which is represented with evenly cyan color segment (Figure 9(b)). Such a profile also implies that the shear stress within the channel is uniform, thereby predicting homogenous cell adhesion within it. Also, when velocity profiles for both original design (labeled with sharp corner) and improved design (labeled with rounded corner) are plotted with MATLAB, rounded corner provides the most uniform cell distribution across the width in the middle of microfluidic as shown in Figure 10.


Computational fluid dynamics modelling of microfluidic channel for dielectrophoretic BioMEMS application.

Low WS, Kadri NA, Abas WA - ScientificWorldJournal (2014)

Comparison of average velocity across the width of microfluidic chamber for rounded corner and sharp corner.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4129156&req=5

fig10: Comparison of average velocity across the width of microfluidic chamber for rounded corner and sharp corner.
Mentions: In contrast to previous design in this study, the surface plot indicates a uniform velocity profile throughout the channel in the first bifurcation, which is represented with evenly cyan color segment (Figure 9(b)). Such a profile also implies that the shear stress within the channel is uniform, thereby predicting homogenous cell adhesion within it. Also, when velocity profiles for both original design (labeled with sharp corner) and improved design (labeled with rounded corner) are plotted with MATLAB, rounded corner provides the most uniform cell distribution across the width in the middle of microfluidic as shown in Figure 10.

Bottom Line: We propose a strategy for optimizing distribution of flow in a typical benchtop microfluidic chamber for dielectrophoretic application.It is aimed at encouraging uniform flow velocity along the whole analysis chamber in order to ensure DEP force is evenly applied to biological particle.The design was validated by hydrodynamic flow simulation using COMSOL Multiphysics v4.2a software.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
We propose a strategy for optimizing distribution of flow in a typical benchtop microfluidic chamber for dielectrophoretic application. It is aimed at encouraging uniform flow velocity along the whole analysis chamber in order to ensure DEP force is evenly applied to biological particle. Via the study, we have come up with a constructive strategy in improving the design of microfluidic channel which will greatly facilitate the use of DEP system in laboratory and primarily focus on the relationship between architecture and cell distribution, by resorting to the tubular structure of blood vessels. The design was validated by hydrodynamic flow simulation using COMSOL Multiphysics v4.2a software. Simulations show that the presence of 2-level bifurcation has developed portioning of volumetric flow which produced uniform flow across the channel. However, further bifurcation will reduce the volumetric flow rate, thus causing undesirable deposition of cell suspension around the chamber. Finally, an improvement of microfluidic design with rounded corner is proposed to encourage a uniform cell adhesion within the channel.

Show MeSH
Related in: MedlinePlus