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Computational fluid dynamics modelling of microfluidic channel for dielectrophoretic BioMEMS application.

Low WS, Kadri NA, Abas WA - ScientificWorldJournal (2014)

Bottom Line: We propose a strategy for optimizing distribution of flow in a typical benchtop microfluidic chamber for dielectrophoretic application.It is aimed at encouraging uniform flow velocity along the whole analysis chamber in order to ensure DEP force is evenly applied to biological particle.The design was validated by hydrodynamic flow simulation using COMSOL Multiphysics v4.2a software.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
We propose a strategy for optimizing distribution of flow in a typical benchtop microfluidic chamber for dielectrophoretic application. It is aimed at encouraging uniform flow velocity along the whole analysis chamber in order to ensure DEP force is evenly applied to biological particle. Via the study, we have come up with a constructive strategy in improving the design of microfluidic channel which will greatly facilitate the use of DEP system in laboratory and primarily focus on the relationship between architecture and cell distribution, by resorting to the tubular structure of blood vessels. The design was validated by hydrodynamic flow simulation using COMSOL Multiphysics v4.2a software. Simulations show that the presence of 2-level bifurcation has developed portioning of volumetric flow which produced uniform flow across the channel. However, further bifurcation will reduce the volumetric flow rate, thus causing undesirable deposition of cell suspension around the chamber. Finally, an improvement of microfluidic design with rounded corner is proposed to encourage a uniform cell adhesion within the channel.

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Related in: MedlinePlus

Schematic of (a) microfluidic channel with rounded corner and its (b) surface velocity plot.
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Related In: Results  -  Collection


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fig9: Schematic of (a) microfluidic channel with rounded corner and its (b) surface velocity plot.

Mentions: By resorting to the study done by Feng et al. [30] and Green et al. [34], a round-shaped turn is identified to generate uniform velocity profiles and cell adhesion within the channel. Thus, the sequence of round-shaped turn is used to replace the sharp corner of our original proposed microfluidic channel architecture. The calculated compactness is 0.51 and its geometrical design is presented in Figure 9(a).


Computational fluid dynamics modelling of microfluidic channel for dielectrophoretic BioMEMS application.

Low WS, Kadri NA, Abas WA - ScientificWorldJournal (2014)

Schematic of (a) microfluidic channel with rounded corner and its (b) surface velocity plot.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4129156&req=5

fig9: Schematic of (a) microfluidic channel with rounded corner and its (b) surface velocity plot.
Mentions: By resorting to the study done by Feng et al. [30] and Green et al. [34], a round-shaped turn is identified to generate uniform velocity profiles and cell adhesion within the channel. Thus, the sequence of round-shaped turn is used to replace the sharp corner of our original proposed microfluidic channel architecture. The calculated compactness is 0.51 and its geometrical design is presented in Figure 9(a).

Bottom Line: We propose a strategy for optimizing distribution of flow in a typical benchtop microfluidic chamber for dielectrophoretic application.It is aimed at encouraging uniform flow velocity along the whole analysis chamber in order to ensure DEP force is evenly applied to biological particle.The design was validated by hydrodynamic flow simulation using COMSOL Multiphysics v4.2a software.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
We propose a strategy for optimizing distribution of flow in a typical benchtop microfluidic chamber for dielectrophoretic application. It is aimed at encouraging uniform flow velocity along the whole analysis chamber in order to ensure DEP force is evenly applied to biological particle. Via the study, we have come up with a constructive strategy in improving the design of microfluidic channel which will greatly facilitate the use of DEP system in laboratory and primarily focus on the relationship between architecture and cell distribution, by resorting to the tubular structure of blood vessels. The design was validated by hydrodynamic flow simulation using COMSOL Multiphysics v4.2a software. Simulations show that the presence of 2-level bifurcation has developed portioning of volumetric flow which produced uniform flow across the channel. However, further bifurcation will reduce the volumetric flow rate, thus causing undesirable deposition of cell suspension around the chamber. Finally, an improvement of microfluidic design with rounded corner is proposed to encourage a uniform cell adhesion within the channel.

Show MeSH
Related in: MedlinePlus