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Computational fluid dynamics modelling of microfluidic channel for dielectrophoretic BioMEMS application.

Low WS, Kadri NA, Abas WA - ScientificWorldJournal (2014)

Bottom Line: We propose a strategy for optimizing distribution of flow in a typical benchtop microfluidic chamber for dielectrophoretic application.It is aimed at encouraging uniform flow velocity along the whole analysis chamber in order to ensure DEP force is evenly applied to biological particle.The design was validated by hydrodynamic flow simulation using COMSOL Multiphysics v4.2a software.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
We propose a strategy for optimizing distribution of flow in a typical benchtop microfluidic chamber for dielectrophoretic application. It is aimed at encouraging uniform flow velocity along the whole analysis chamber in order to ensure DEP force is evenly applied to biological particle. Via the study, we have come up with a constructive strategy in improving the design of microfluidic channel which will greatly facilitate the use of DEP system in laboratory and primarily focus on the relationship between architecture and cell distribution, by resorting to the tubular structure of blood vessels. The design was validated by hydrodynamic flow simulation using COMSOL Multiphysics v4.2a software. Simulations show that the presence of 2-level bifurcation has developed portioning of volumetric flow which produced uniform flow across the channel. However, further bifurcation will reduce the volumetric flow rate, thus causing undesirable deposition of cell suspension around the chamber. Finally, an improvement of microfluidic design with rounded corner is proposed to encourage a uniform cell adhesion within the channel.

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Schematic presentation of velocity flow at sharp corner of microfluidic channel.
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fig8: Schematic presentation of velocity flow at sharp corner of microfluidic channel.

Mentions: Generally, the basic proposed microfluidic structure with 2-level of bifurcation and 1 : 1 subchannel length ratio appears to be the best performance to process a uniform velocity flow across the channel. In spite of the fact that a rectangular cross-sectional microfluidic channel is easy to fabricate, multiple zero velocity areas are normally produced in the corners. It is further evidenced through COMSOL simulation as presented in Figure 8, where the dark blue colour segment around the sharp corner represents a zero velocity profile. This situation can cause the large number of cells to be collected [34]. The lack of uniform adhesion in this region compared to others indicates that the channels with sharp turns are not optimal for DEP cell separation application.


Computational fluid dynamics modelling of microfluidic channel for dielectrophoretic BioMEMS application.

Low WS, Kadri NA, Abas WA - ScientificWorldJournal (2014)

Schematic presentation of velocity flow at sharp corner of microfluidic channel.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4129156&req=5

fig8: Schematic presentation of velocity flow at sharp corner of microfluidic channel.
Mentions: Generally, the basic proposed microfluidic structure with 2-level of bifurcation and 1 : 1 subchannel length ratio appears to be the best performance to process a uniform velocity flow across the channel. In spite of the fact that a rectangular cross-sectional microfluidic channel is easy to fabricate, multiple zero velocity areas are normally produced in the corners. It is further evidenced through COMSOL simulation as presented in Figure 8, where the dark blue colour segment around the sharp corner represents a zero velocity profile. This situation can cause the large number of cells to be collected [34]. The lack of uniform adhesion in this region compared to others indicates that the channels with sharp turns are not optimal for DEP cell separation application.

Bottom Line: We propose a strategy for optimizing distribution of flow in a typical benchtop microfluidic chamber for dielectrophoretic application.It is aimed at encouraging uniform flow velocity along the whole analysis chamber in order to ensure DEP force is evenly applied to biological particle.The design was validated by hydrodynamic flow simulation using COMSOL Multiphysics v4.2a software.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
We propose a strategy for optimizing distribution of flow in a typical benchtop microfluidic chamber for dielectrophoretic application. It is aimed at encouraging uniform flow velocity along the whole analysis chamber in order to ensure DEP force is evenly applied to biological particle. Via the study, we have come up with a constructive strategy in improving the design of microfluidic channel which will greatly facilitate the use of DEP system in laboratory and primarily focus on the relationship between architecture and cell distribution, by resorting to the tubular structure of blood vessels. The design was validated by hydrodynamic flow simulation using COMSOL Multiphysics v4.2a software. Simulations show that the presence of 2-level bifurcation has developed portioning of volumetric flow which produced uniform flow across the channel. However, further bifurcation will reduce the volumetric flow rate, thus causing undesirable deposition of cell suspension around the chamber. Finally, an improvement of microfluidic design with rounded corner is proposed to encourage a uniform cell adhesion within the channel.

Show MeSH
Related in: MedlinePlus