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Computational fluid dynamics modelling of microfluidic channel for dielectrophoretic BioMEMS application.

Low WS, Kadri NA, Abas WA - ScientificWorldJournal (2014)

Bottom Line: We propose a strategy for optimizing distribution of flow in a typical benchtop microfluidic chamber for dielectrophoretic application.It is aimed at encouraging uniform flow velocity along the whole analysis chamber in order to ensure DEP force is evenly applied to biological particle.The design was validated by hydrodynamic flow simulation using COMSOL Multiphysics v4.2a software.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
We propose a strategy for optimizing distribution of flow in a typical benchtop microfluidic chamber for dielectrophoretic application. It is aimed at encouraging uniform flow velocity along the whole analysis chamber in order to ensure DEP force is evenly applied to biological particle. Via the study, we have come up with a constructive strategy in improving the design of microfluidic channel which will greatly facilitate the use of DEP system in laboratory and primarily focus on the relationship between architecture and cell distribution, by resorting to the tubular structure of blood vessels. The design was validated by hydrodynamic flow simulation using COMSOL Multiphysics v4.2a software. Simulations show that the presence of 2-level bifurcation has developed portioning of volumetric flow which produced uniform flow across the channel. However, further bifurcation will reduce the volumetric flow rate, thus causing undesirable deposition of cell suspension around the chamber. Finally, an improvement of microfluidic design with rounded corner is proposed to encourage a uniform cell adhesion within the channel.

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Related in: MedlinePlus

Graph of velocity versus arc length in the middle of microfluidic chamber (subchannel length ratio 1 : 2 & 2 : 1).
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Related In: Results  -  Collection


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fig6: Graph of velocity versus arc length in the middle of microfluidic chamber (subchannel length ratio 1 : 2 & 2 : 1).

Mentions: In general, the surface velocity plot and arrow plot for both architectures indicate a uniform distribution across the channel. However, in the process of further validating the result, an analysis about velocity fluctuation in the middle of both microfluidic channels is implemented. As COMSOL Multiphysics software merely provides individual design analysis, the velocity magnitude data are exported from COMSOL in order to compare the velocity flow within both architectures. The data are then plotted by using MATLAB R2010a software. The graph of average velocity field in the middle of the analysis area for subchannel with length ratio of 1 : 2 and 2 : 1 is delivered in Figure 6. To simplify the explanation, microfluidic with subchannel's length ratio of 1 : 2 is represented with Model A while microfluidic with subchannel's lengh ratio of 2 : 1 is Model B.


Computational fluid dynamics modelling of microfluidic channel for dielectrophoretic BioMEMS application.

Low WS, Kadri NA, Abas WA - ScientificWorldJournal (2014)

Graph of velocity versus arc length in the middle of microfluidic chamber (subchannel length ratio 1 : 2 & 2 : 1).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4129156&req=5

fig6: Graph of velocity versus arc length in the middle of microfluidic chamber (subchannel length ratio 1 : 2 & 2 : 1).
Mentions: In general, the surface velocity plot and arrow plot for both architectures indicate a uniform distribution across the channel. However, in the process of further validating the result, an analysis about velocity fluctuation in the middle of both microfluidic channels is implemented. As COMSOL Multiphysics software merely provides individual design analysis, the velocity magnitude data are exported from COMSOL in order to compare the velocity flow within both architectures. The data are then plotted by using MATLAB R2010a software. The graph of average velocity field in the middle of the analysis area for subchannel with length ratio of 1 : 2 and 2 : 1 is delivered in Figure 6. To simplify the explanation, microfluidic with subchannel's length ratio of 1 : 2 is represented with Model A while microfluidic with subchannel's lengh ratio of 2 : 1 is Model B.

Bottom Line: We propose a strategy for optimizing distribution of flow in a typical benchtop microfluidic chamber for dielectrophoretic application.It is aimed at encouraging uniform flow velocity along the whole analysis chamber in order to ensure DEP force is evenly applied to biological particle.The design was validated by hydrodynamic flow simulation using COMSOL Multiphysics v4.2a software.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
We propose a strategy for optimizing distribution of flow in a typical benchtop microfluidic chamber for dielectrophoretic application. It is aimed at encouraging uniform flow velocity along the whole analysis chamber in order to ensure DEP force is evenly applied to biological particle. Via the study, we have come up with a constructive strategy in improving the design of microfluidic channel which will greatly facilitate the use of DEP system in laboratory and primarily focus on the relationship between architecture and cell distribution, by resorting to the tubular structure of blood vessels. The design was validated by hydrodynamic flow simulation using COMSOL Multiphysics v4.2a software. Simulations show that the presence of 2-level bifurcation has developed portioning of volumetric flow which produced uniform flow across the channel. However, further bifurcation will reduce the volumetric flow rate, thus causing undesirable deposition of cell suspension around the chamber. Finally, an improvement of microfluidic design with rounded corner is proposed to encourage a uniform cell adhesion within the channel.

Show MeSH
Related in: MedlinePlus