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Assessment of the effect of Schistosoma haematobium co infection on malaria parasites and immune responses in rural populations in Gabon: study protocol.

Ateba Ngoa U, Zinsou JF, Kassa RF, Ngoune Feugap E, Honkpehedji YJ, Massinga-Loembe M, Kenguele Moundounga H, Nkoma Mouima AM, Mbenkep LH, Wammes LJ, Mbow M, Kruize Y, Mombo-Ngoma G, Bouyoukou Hounkpatin AL, Dejon Agobe JC, Saadou I, Lell B, Smits H, Kremsner PG, Yazdanbakhsh M, Adegnika AA - Springerplus (2014)

Bottom Line: Participants, 5 to 50 years old, will be enrolled and grouped according to their infection status.The level of antibody specific to Plasmodium falciparum blood stage and gametocyte antigens will be measured using ELISA.PBMC will be isolated for phenotyping of different T cell subsets ex vivo by flow cytometry and for culture and cytokine response assessment.

View Article: PubMed Central - PubMed

Affiliation: Centre de Recherches Médicales de Lambaréné, BP: 118, Lambaréné, Gabon ; Institut für Tropenmedizin, Universität Tübingen, Wilhelmstraβe 27, D-72074 Tübingen, Germany ; Department of Parasitology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.

ABSTRACT

Background: Malaria and helminth co infection are common in tropical and subtropical areas where they affect the life of millions of people. While both helminth and malaria parasites have immunomodulatory activities, little is known about the consequence of co-infections on malaria antigen specific immune responses.

Method/design: This study will be conducted in two rural areas of the Moyen Ogooué province in Gabon, endemic for both Plasmodium falciparum and Schistosoma haematobium infections. Participants, 5 to 50 years old, will be enrolled and grouped according to their infection status. S. haematobium and malaria parasites will be detected, demographic and clinical data will be recorded and blood will be collected for hematological as well as for immunological assays. The level of antibody specific to Plasmodium falciparum blood stage and gametocyte antigens will be measured using ELISA. PBMC will be isolated for phenotyping of different T cell subsets ex vivo by flow cytometry and for culture and cytokine response assessment.

Discussion: We will provide a comprehensive picture of the interaction between schistosomes and malaria parasites which co-localize in peripheral blood. We will test the hypothesis that schistosome infection has an impact on specific humoral as well as on cellular immune responses to malaria antigens.

No MeSH data available.


Related in: MedlinePlus

Kernel density estimation ofSchistosoma haemtobiuminfection per age in the the Pk15 area and the Bindo village. This estimation is based on results of a random sample of around 10% of the population of both area.
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Fig4: Kernel density estimation ofSchistosoma haemtobiuminfection per age in the the Pk15 area and the Bindo village. This estimation is based on results of a random sample of around 10% of the population of both area.

Mentions: Pilot studies have been conducted in the PK15 area and the Bindo village to characterize the study population and to set up the study procedure. All houses in both areas have been identified and their GPS coordinates recorded (Figure 2). To obtain demographic data and to establish the epidemiological feature of S. haematobium infection in the study area a random selection of houses has been screened to represent around 10% of the population. Typical to low income countries the age pyramid curve presented a broad base as shown in Figure 3. In both areas the age pyramids show that only few adults from 20 to 49 are living in the village. This could be due to the migration of young adults to big cities for work or for study purposes. In Bindo this is less apparent as inhabitants are employed by the palm oil company. Regarding the S. haematobium infection we found an overall prevalence of 43% in the PK15 area and 15% in Bindo village. This difference between the two areas could be explained by the fact that in the Pk15 area streams represent the first source of water compared to the Bindo village where public water pumps are available. As represented in the age prevalence curve, children were the most infected by S. haematobium and showed the highest infection intensity (Figures 4 and 5).Figure 2


Assessment of the effect of Schistosoma haematobium co infection on malaria parasites and immune responses in rural populations in Gabon: study protocol.

Ateba Ngoa U, Zinsou JF, Kassa RF, Ngoune Feugap E, Honkpehedji YJ, Massinga-Loembe M, Kenguele Moundounga H, Nkoma Mouima AM, Mbenkep LH, Wammes LJ, Mbow M, Kruize Y, Mombo-Ngoma G, Bouyoukou Hounkpatin AL, Dejon Agobe JC, Saadou I, Lell B, Smits H, Kremsner PG, Yazdanbakhsh M, Adegnika AA - Springerplus (2014)

Kernel density estimation ofSchistosoma haemtobiuminfection per age in the the Pk15 area and the Bindo village. This estimation is based on results of a random sample of around 10% of the population of both area.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4128953&req=5

Fig4: Kernel density estimation ofSchistosoma haemtobiuminfection per age in the the Pk15 area and the Bindo village. This estimation is based on results of a random sample of around 10% of the population of both area.
Mentions: Pilot studies have been conducted in the PK15 area and the Bindo village to characterize the study population and to set up the study procedure. All houses in both areas have been identified and their GPS coordinates recorded (Figure 2). To obtain demographic data and to establish the epidemiological feature of S. haematobium infection in the study area a random selection of houses has been screened to represent around 10% of the population. Typical to low income countries the age pyramid curve presented a broad base as shown in Figure 3. In both areas the age pyramids show that only few adults from 20 to 49 are living in the village. This could be due to the migration of young adults to big cities for work or for study purposes. In Bindo this is less apparent as inhabitants are employed by the palm oil company. Regarding the S. haematobium infection we found an overall prevalence of 43% in the PK15 area and 15% in Bindo village. This difference between the two areas could be explained by the fact that in the Pk15 area streams represent the first source of water compared to the Bindo village where public water pumps are available. As represented in the age prevalence curve, children were the most infected by S. haematobium and showed the highest infection intensity (Figures 4 and 5).Figure 2

Bottom Line: Participants, 5 to 50 years old, will be enrolled and grouped according to their infection status.The level of antibody specific to Plasmodium falciparum blood stage and gametocyte antigens will be measured using ELISA.PBMC will be isolated for phenotyping of different T cell subsets ex vivo by flow cytometry and for culture and cytokine response assessment.

View Article: PubMed Central - PubMed

Affiliation: Centre de Recherches Médicales de Lambaréné, BP: 118, Lambaréné, Gabon ; Institut für Tropenmedizin, Universität Tübingen, Wilhelmstraβe 27, D-72074 Tübingen, Germany ; Department of Parasitology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.

ABSTRACT

Background: Malaria and helminth co infection are common in tropical and subtropical areas where they affect the life of millions of people. While both helminth and malaria parasites have immunomodulatory activities, little is known about the consequence of co-infections on malaria antigen specific immune responses.

Method/design: This study will be conducted in two rural areas of the Moyen Ogooué province in Gabon, endemic for both Plasmodium falciparum and Schistosoma haematobium infections. Participants, 5 to 50 years old, will be enrolled and grouped according to their infection status. S. haematobium and malaria parasites will be detected, demographic and clinical data will be recorded and blood will be collected for hematological as well as for immunological assays. The level of antibody specific to Plasmodium falciparum blood stage and gametocyte antigens will be measured using ELISA. PBMC will be isolated for phenotyping of different T cell subsets ex vivo by flow cytometry and for culture and cytokine response assessment.

Discussion: We will provide a comprehensive picture of the interaction between schistosomes and malaria parasites which co-localize in peripheral blood. We will test the hypothesis that schistosome infection has an impact on specific humoral as well as on cellular immune responses to malaria antigens.

No MeSH data available.


Related in: MedlinePlus