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Evaluation of the ecotoxicity of sediments from Yangtze river estuary and contribution of priority PAHs to ah receptor--mediated activities.

Liu L, Chen L, Shao Y, Zhang L, Floehr T, Xiao H, Yan Y, Eichbaum K, Hollert H, Wu L - PLoS ONE (2014)

Bottom Line: The cytotoxicity and AhR-mediated activity of sediments from the Yangtze River estuary ranged from low level to moderate level compared with the ecotoxicity of sediments from other river systems.However, Yangtze River releases approximately 14 times greater water discharge compared with Rhine, a major river in Europe.Based on the results of mass balance calculation of chemical toxic equivalent concentrations (TEQs), our conclusion is that priority polycyclic aromatic hydrocarbons indicated a low portion of bio-TEQs ranging from 1% to 10% of crude extracts.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Yangtze Water environment, Ministry of Education, Tongji University, Shanghai, China.

ABSTRACT
In this study, in vitro bioassays were performed to assess the ecotoxicological potential of sediments from Yangtze River estuary. The cytotoxicity and aryl hydrocarbon receptor (AhR)-mediated toxicity of sediment extracts with rainbow trout (Oncorhynchus mykiss) liver cells were determined by neutral red retention and 7-ethoxyresorufin-O-deethylase assays. The cytotoxicity and AhR-mediated activity of sediments from the Yangtze River estuary ranged from low level to moderate level compared with the ecotoxicity of sediments from other river systems. However, Yangtze River releases approximately 14 times greater water discharge compared with Rhine, a major river in Europe. Thus, the absolute pollution mass transfer of Yangtze River may be detrimental to the environmental quality of estuary and East China Sea. Effect-directed analysis was applied to identify substances causing high dioxin-like activities. To identify unknown substances contributing to dioxin-like potencies of whole extracts, we fractionated crude extracts by open column chromatography. Non-polar paraffinic components (F1), weakly and moderately polar components (F2), and highly polar substances (F3) were separated from each crude extract of sediments. F2 showed the highest dioxin-like activities. Based on the results of mass balance calculation of chemical toxic equivalent concentrations (TEQs), our conclusion is that priority polycyclic aromatic hydrocarbons indicated a low portion of bio-TEQs ranging from 1% to 10% of crude extracts. Further studies should be conducted to identify unknown pollutants.

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Related in: MedlinePlus

AhR-mediated activities of crude and multilayer fractions of sediment extracts from the Yangtze River estuary.The results are based on EROD inductions with RTL-W1 cells and presented as bio-TEQs. F1 contains non-polar paraffinic components. F2 contains weakly and moderately polar components. F3 contains highly polar components. The numbers in percentage were calculated as (F1+F2+F3–CE)/CE×100%. The difference between the combined F1 to F3 and the induced crude extracts induction is given. Bio-TEQs are given as means of n = 3 independent experiments. CE, crude extracts.
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pone-0104748-g004: AhR-mediated activities of crude and multilayer fractions of sediment extracts from the Yangtze River estuary.The results are based on EROD inductions with RTL-W1 cells and presented as bio-TEQs. F1 contains non-polar paraffinic components. F2 contains weakly and moderately polar components. F3 contains highly polar components. The numbers in percentage were calculated as (F1+F2+F3–CE)/CE×100%. The difference between the combined F1 to F3 and the induced crude extracts induction is given. Bio-TEQs are given as means of n = 3 independent experiments. CE, crude extracts.

Mentions: Based on EROD induction assay results of the crude extracts, the sediment samples from sites Y2, Y4, Y5, Y7, Y8, and Y9, which had the highest bio-TEQ values, were selected for further identification of AhR-mediated activities using multilayer fractionation. As shown in Figure 4, AhR-mediated activities caused by multilayer fractions (F1 to F3) were compared with crude sediment extract inductions.


Evaluation of the ecotoxicity of sediments from Yangtze river estuary and contribution of priority PAHs to ah receptor--mediated activities.

Liu L, Chen L, Shao Y, Zhang L, Floehr T, Xiao H, Yan Y, Eichbaum K, Hollert H, Wu L - PLoS ONE (2014)

AhR-mediated activities of crude and multilayer fractions of sediment extracts from the Yangtze River estuary.The results are based on EROD inductions with RTL-W1 cells and presented as bio-TEQs. F1 contains non-polar paraffinic components. F2 contains weakly and moderately polar components. F3 contains highly polar components. The numbers in percentage were calculated as (F1+F2+F3–CE)/CE×100%. The difference between the combined F1 to F3 and the induced crude extracts induction is given. Bio-TEQs are given as means of n = 3 independent experiments. CE, crude extracts.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4128779&req=5

pone-0104748-g004: AhR-mediated activities of crude and multilayer fractions of sediment extracts from the Yangtze River estuary.The results are based on EROD inductions with RTL-W1 cells and presented as bio-TEQs. F1 contains non-polar paraffinic components. F2 contains weakly and moderately polar components. F3 contains highly polar components. The numbers in percentage were calculated as (F1+F2+F3–CE)/CE×100%. The difference between the combined F1 to F3 and the induced crude extracts induction is given. Bio-TEQs are given as means of n = 3 independent experiments. CE, crude extracts.
Mentions: Based on EROD induction assay results of the crude extracts, the sediment samples from sites Y2, Y4, Y5, Y7, Y8, and Y9, which had the highest bio-TEQ values, were selected for further identification of AhR-mediated activities using multilayer fractionation. As shown in Figure 4, AhR-mediated activities caused by multilayer fractions (F1 to F3) were compared with crude sediment extract inductions.

Bottom Line: The cytotoxicity and AhR-mediated activity of sediments from the Yangtze River estuary ranged from low level to moderate level compared with the ecotoxicity of sediments from other river systems.However, Yangtze River releases approximately 14 times greater water discharge compared with Rhine, a major river in Europe.Based on the results of mass balance calculation of chemical toxic equivalent concentrations (TEQs), our conclusion is that priority polycyclic aromatic hydrocarbons indicated a low portion of bio-TEQs ranging from 1% to 10% of crude extracts.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Yangtze Water environment, Ministry of Education, Tongji University, Shanghai, China.

ABSTRACT
In this study, in vitro bioassays were performed to assess the ecotoxicological potential of sediments from Yangtze River estuary. The cytotoxicity and aryl hydrocarbon receptor (AhR)-mediated toxicity of sediment extracts with rainbow trout (Oncorhynchus mykiss) liver cells were determined by neutral red retention and 7-ethoxyresorufin-O-deethylase assays. The cytotoxicity and AhR-mediated activity of sediments from the Yangtze River estuary ranged from low level to moderate level compared with the ecotoxicity of sediments from other river systems. However, Yangtze River releases approximately 14 times greater water discharge compared with Rhine, a major river in Europe. Thus, the absolute pollution mass transfer of Yangtze River may be detrimental to the environmental quality of estuary and East China Sea. Effect-directed analysis was applied to identify substances causing high dioxin-like activities. To identify unknown substances contributing to dioxin-like potencies of whole extracts, we fractionated crude extracts by open column chromatography. Non-polar paraffinic components (F1), weakly and moderately polar components (F2), and highly polar substances (F3) were separated from each crude extract of sediments. F2 showed the highest dioxin-like activities. Based on the results of mass balance calculation of chemical toxic equivalent concentrations (TEQs), our conclusion is that priority polycyclic aromatic hydrocarbons indicated a low portion of bio-TEQs ranging from 1% to 10% of crude extracts. Further studies should be conducted to identify unknown pollutants.

Show MeSH
Related in: MedlinePlus