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Evaluation of the ecotoxicity of sediments from Yangtze river estuary and contribution of priority PAHs to ah receptor--mediated activities.

Liu L, Chen L, Shao Y, Zhang L, Floehr T, Xiao H, Yan Y, Eichbaum K, Hollert H, Wu L - PLoS ONE (2014)

Bottom Line: The cytotoxicity and AhR-mediated activity of sediments from the Yangtze River estuary ranged from low level to moderate level compared with the ecotoxicity of sediments from other river systems.However, Yangtze River releases approximately 14 times greater water discharge compared with Rhine, a major river in Europe.Based on the results of mass balance calculation of chemical toxic equivalent concentrations (TEQs), our conclusion is that priority polycyclic aromatic hydrocarbons indicated a low portion of bio-TEQs ranging from 1% to 10% of crude extracts.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Yangtze Water environment, Ministry of Education, Tongji University, Shanghai, China.

ABSTRACT
In this study, in vitro bioassays were performed to assess the ecotoxicological potential of sediments from Yangtze River estuary. The cytotoxicity and aryl hydrocarbon receptor (AhR)-mediated toxicity of sediment extracts with rainbow trout (Oncorhynchus mykiss) liver cells were determined by neutral red retention and 7-ethoxyresorufin-O-deethylase assays. The cytotoxicity and AhR-mediated activity of sediments from the Yangtze River estuary ranged from low level to moderate level compared with the ecotoxicity of sediments from other river systems. However, Yangtze River releases approximately 14 times greater water discharge compared with Rhine, a major river in Europe. Thus, the absolute pollution mass transfer of Yangtze River may be detrimental to the environmental quality of estuary and East China Sea. Effect-directed analysis was applied to identify substances causing high dioxin-like activities. To identify unknown substances contributing to dioxin-like potencies of whole extracts, we fractionated crude extracts by open column chromatography. Non-polar paraffinic components (F1), weakly and moderately polar components (F2), and highly polar substances (F3) were separated from each crude extract of sediments. F2 showed the highest dioxin-like activities. Based on the results of mass balance calculation of chemical toxic equivalent concentrations (TEQs), our conclusion is that priority polycyclic aromatic hydrocarbons indicated a low portion of bio-TEQs ranging from 1% to 10% of crude extracts. Further studies should be conducted to identify unknown pollutants.

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Cytotoxicity of sediment extracts from the Yangtze River estuary.n.d. = not detectable, indicating that no toxic effect of sediment extracts at a concentration of 100 mg/mL with RTL-W1 cells. NR50 values for sediment extracts are given in mg sediment equivalent per mL medium (mg/mL). All the values are expressed as means ± SD.
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pone-0104748-g002: Cytotoxicity of sediment extracts from the Yangtze River estuary.n.d. = not detectable, indicating that no toxic effect of sediment extracts at a concentration of 100 mg/mL with RTL-W1 cells. NR50 values for sediment extracts are given in mg sediment equivalent per mL medium (mg/mL). All the values are expressed as means ± SD.

Mentions: The results of the NR assay on the crude extracts are shown in Figure 2. The sediment extracts from the study sites Y1, Y2, and Y3 showed very low potency and failed to elicit cytotoxic effects at a concentration of 100 mg/mL. By contrast, extracts from the six other sites revealed evident cytotoxicity to RTL-W1 cells with NR50 ranging from 4.1 mg/mL to 43.4 mg/mL (Table S2). The extracts from sites Y5, Y8, and Y9 revealed relatively greater cytotoxicity than those from other sites. The lowest cytotoxic potency was obtained from extracts collected from sites Y4, Y6, and Y7. The cytotoxicity of the sediment extracts from downstream regions generally showed higher activities than those from upstream regions.


Evaluation of the ecotoxicity of sediments from Yangtze river estuary and contribution of priority PAHs to ah receptor--mediated activities.

Liu L, Chen L, Shao Y, Zhang L, Floehr T, Xiao H, Yan Y, Eichbaum K, Hollert H, Wu L - PLoS ONE (2014)

Cytotoxicity of sediment extracts from the Yangtze River estuary.n.d. = not detectable, indicating that no toxic effect of sediment extracts at a concentration of 100 mg/mL with RTL-W1 cells. NR50 values for sediment extracts are given in mg sediment equivalent per mL medium (mg/mL). All the values are expressed as means ± SD.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4128779&req=5

pone-0104748-g002: Cytotoxicity of sediment extracts from the Yangtze River estuary.n.d. = not detectable, indicating that no toxic effect of sediment extracts at a concentration of 100 mg/mL with RTL-W1 cells. NR50 values for sediment extracts are given in mg sediment equivalent per mL medium (mg/mL). All the values are expressed as means ± SD.
Mentions: The results of the NR assay on the crude extracts are shown in Figure 2. The sediment extracts from the study sites Y1, Y2, and Y3 showed very low potency and failed to elicit cytotoxic effects at a concentration of 100 mg/mL. By contrast, extracts from the six other sites revealed evident cytotoxicity to RTL-W1 cells with NR50 ranging from 4.1 mg/mL to 43.4 mg/mL (Table S2). The extracts from sites Y5, Y8, and Y9 revealed relatively greater cytotoxicity than those from other sites. The lowest cytotoxic potency was obtained from extracts collected from sites Y4, Y6, and Y7. The cytotoxicity of the sediment extracts from downstream regions generally showed higher activities than those from upstream regions.

Bottom Line: The cytotoxicity and AhR-mediated activity of sediments from the Yangtze River estuary ranged from low level to moderate level compared with the ecotoxicity of sediments from other river systems.However, Yangtze River releases approximately 14 times greater water discharge compared with Rhine, a major river in Europe.Based on the results of mass balance calculation of chemical toxic equivalent concentrations (TEQs), our conclusion is that priority polycyclic aromatic hydrocarbons indicated a low portion of bio-TEQs ranging from 1% to 10% of crude extracts.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Yangtze Water environment, Ministry of Education, Tongji University, Shanghai, China.

ABSTRACT
In this study, in vitro bioassays were performed to assess the ecotoxicological potential of sediments from Yangtze River estuary. The cytotoxicity and aryl hydrocarbon receptor (AhR)-mediated toxicity of sediment extracts with rainbow trout (Oncorhynchus mykiss) liver cells were determined by neutral red retention and 7-ethoxyresorufin-O-deethylase assays. The cytotoxicity and AhR-mediated activity of sediments from the Yangtze River estuary ranged from low level to moderate level compared with the ecotoxicity of sediments from other river systems. However, Yangtze River releases approximately 14 times greater water discharge compared with Rhine, a major river in Europe. Thus, the absolute pollution mass transfer of Yangtze River may be detrimental to the environmental quality of estuary and East China Sea. Effect-directed analysis was applied to identify substances causing high dioxin-like activities. To identify unknown substances contributing to dioxin-like potencies of whole extracts, we fractionated crude extracts by open column chromatography. Non-polar paraffinic components (F1), weakly and moderately polar components (F2), and highly polar substances (F3) were separated from each crude extract of sediments. F2 showed the highest dioxin-like activities. Based on the results of mass balance calculation of chemical toxic equivalent concentrations (TEQs), our conclusion is that priority polycyclic aromatic hydrocarbons indicated a low portion of bio-TEQs ranging from 1% to 10% of crude extracts. Further studies should be conducted to identify unknown pollutants.

Show MeSH
Related in: MedlinePlus