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Evaluation of the ecotoxicity of sediments from Yangtze river estuary and contribution of priority PAHs to ah receptor--mediated activities.

Liu L, Chen L, Shao Y, Zhang L, Floehr T, Xiao H, Yan Y, Eichbaum K, Hollert H, Wu L - PLoS ONE (2014)

Bottom Line: The cytotoxicity and AhR-mediated activity of sediments from the Yangtze River estuary ranged from low level to moderate level compared with the ecotoxicity of sediments from other river systems.However, Yangtze River releases approximately 14 times greater water discharge compared with Rhine, a major river in Europe.Based on the results of mass balance calculation of chemical toxic equivalent concentrations (TEQs), our conclusion is that priority polycyclic aromatic hydrocarbons indicated a low portion of bio-TEQs ranging from 1% to 10% of crude extracts.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Yangtze Water environment, Ministry of Education, Tongji University, Shanghai, China.

ABSTRACT
In this study, in vitro bioassays were performed to assess the ecotoxicological potential of sediments from Yangtze River estuary. The cytotoxicity and aryl hydrocarbon receptor (AhR)-mediated toxicity of sediment extracts with rainbow trout (Oncorhynchus mykiss) liver cells were determined by neutral red retention and 7-ethoxyresorufin-O-deethylase assays. The cytotoxicity and AhR-mediated activity of sediments from the Yangtze River estuary ranged from low level to moderate level compared with the ecotoxicity of sediments from other river systems. However, Yangtze River releases approximately 14 times greater water discharge compared with Rhine, a major river in Europe. Thus, the absolute pollution mass transfer of Yangtze River may be detrimental to the environmental quality of estuary and East China Sea. Effect-directed analysis was applied to identify substances causing high dioxin-like activities. To identify unknown substances contributing to dioxin-like potencies of whole extracts, we fractionated crude extracts by open column chromatography. Non-polar paraffinic components (F1), weakly and moderately polar components (F2), and highly polar substances (F3) were separated from each crude extract of sediments. F2 showed the highest dioxin-like activities. Based on the results of mass balance calculation of chemical toxic equivalent concentrations (TEQs), our conclusion is that priority polycyclic aromatic hydrocarbons indicated a low portion of bio-TEQs ranging from 1% to 10% of crude extracts. Further studies should be conducted to identify unknown pollutants.

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Related in: MedlinePlus

Map of the sampling locations in the Yangtze River estuary.Nine samples Y1–Y9 were collected along the estuary in March 2012.
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pone-0104748-g001: Map of the sampling locations in the Yangtze River estuary.Nine samples Y1–Y9 were collected along the estuary in March 2012.

Mentions: No specific permissions were required for the completion of this study as the field measurements did not involve endangered or protected species nor were conducted in a specified protected area. The surface layer of sediment samples (0 cm to 5 cm) were collected from nine locations in the Yangtze River estuary in March 2012. For each sampling locations, four samples was collected and then mixed to form one sample. The sampling locations are shown in Figure 1. Samples were collected along the salinity gradient and location information is presented in Table S1. Samples Y1 to Y3 were fresh water dominated sediments. Sites Y4 and Y5 were located in the turbidity maximum zone and the samples were brackish water dominated sediments. Sites Y6 to Y9 were located in the river plume zone and the samples were marine sediments. Sediments were collected from each location by using a stainless steel grab sampler. Samples were shock-frozen at –20°C immediately until further processing. Afterward, these samples were freeze-dried at –50°C and sieved using a 100-mesh sieve. The samples were then stored in combusted glass with Teflon lined lids at –20°C in the dark until extraction.


Evaluation of the ecotoxicity of sediments from Yangtze river estuary and contribution of priority PAHs to ah receptor--mediated activities.

Liu L, Chen L, Shao Y, Zhang L, Floehr T, Xiao H, Yan Y, Eichbaum K, Hollert H, Wu L - PLoS ONE (2014)

Map of the sampling locations in the Yangtze River estuary.Nine samples Y1–Y9 were collected along the estuary in March 2012.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4128779&req=5

pone-0104748-g001: Map of the sampling locations in the Yangtze River estuary.Nine samples Y1–Y9 were collected along the estuary in March 2012.
Mentions: No specific permissions were required for the completion of this study as the field measurements did not involve endangered or protected species nor were conducted in a specified protected area. The surface layer of sediment samples (0 cm to 5 cm) were collected from nine locations in the Yangtze River estuary in March 2012. For each sampling locations, four samples was collected and then mixed to form one sample. The sampling locations are shown in Figure 1. Samples were collected along the salinity gradient and location information is presented in Table S1. Samples Y1 to Y3 were fresh water dominated sediments. Sites Y4 and Y5 were located in the turbidity maximum zone and the samples were brackish water dominated sediments. Sites Y6 to Y9 were located in the river plume zone and the samples were marine sediments. Sediments were collected from each location by using a stainless steel grab sampler. Samples were shock-frozen at –20°C immediately until further processing. Afterward, these samples were freeze-dried at –50°C and sieved using a 100-mesh sieve. The samples were then stored in combusted glass with Teflon lined lids at –20°C in the dark until extraction.

Bottom Line: The cytotoxicity and AhR-mediated activity of sediments from the Yangtze River estuary ranged from low level to moderate level compared with the ecotoxicity of sediments from other river systems.However, Yangtze River releases approximately 14 times greater water discharge compared with Rhine, a major river in Europe.Based on the results of mass balance calculation of chemical toxic equivalent concentrations (TEQs), our conclusion is that priority polycyclic aromatic hydrocarbons indicated a low portion of bio-TEQs ranging from 1% to 10% of crude extracts.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Yangtze Water environment, Ministry of Education, Tongji University, Shanghai, China.

ABSTRACT
In this study, in vitro bioassays were performed to assess the ecotoxicological potential of sediments from Yangtze River estuary. The cytotoxicity and aryl hydrocarbon receptor (AhR)-mediated toxicity of sediment extracts with rainbow trout (Oncorhynchus mykiss) liver cells were determined by neutral red retention and 7-ethoxyresorufin-O-deethylase assays. The cytotoxicity and AhR-mediated activity of sediments from the Yangtze River estuary ranged from low level to moderate level compared with the ecotoxicity of sediments from other river systems. However, Yangtze River releases approximately 14 times greater water discharge compared with Rhine, a major river in Europe. Thus, the absolute pollution mass transfer of Yangtze River may be detrimental to the environmental quality of estuary and East China Sea. Effect-directed analysis was applied to identify substances causing high dioxin-like activities. To identify unknown substances contributing to dioxin-like potencies of whole extracts, we fractionated crude extracts by open column chromatography. Non-polar paraffinic components (F1), weakly and moderately polar components (F2), and highly polar substances (F3) were separated from each crude extract of sediments. F2 showed the highest dioxin-like activities. Based on the results of mass balance calculation of chemical toxic equivalent concentrations (TEQs), our conclusion is that priority polycyclic aromatic hydrocarbons indicated a low portion of bio-TEQs ranging from 1% to 10% of crude extracts. Further studies should be conducted to identify unknown pollutants.

Show MeSH
Related in: MedlinePlus