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Pleiotropic functions for transcription factor zscan10.

Kraus P, V S, Yu HB, Xing X, Lim SL, Adler T, Pimentel JA, Becker L, Bohla A, Garrett L, Hans W, Hölter SM, Janas E, Moreth K, Prehn C, Puk O, Rathkolb B, Rozman J, Adamski J, Bekeredjian R, Busch DH, Graw J, Klingenspor M, Klopstock T, Neff F, Ollert M, Stoeger T, Yildrim AÖ, Eickelberg O, Wolf E, Wurst W, Fuchs H, Gailus-Durner V, de Angelis MH, Lufkin T, Stanton LW - PLoS ONE (2014)

Bottom Line: Here we suggest a potential role of Zscan10 in controlling progenitor cell populations in vivo.Mice homozygous for a Zscan10 mutation exhibit reduced weight, mild hypoplasia in the spleen, heart and long bones and phenocopy an eye malformation previously described for Sox2 hypomorphs.Phenotypic abnormalities are supported by the nature of Zscan10 expression in midgestation embryos and adults suggesting a role for Zscan10 in either maintaining progenitor cell subpopulation or impacting on fate choice decisions thereof.

View Article: PubMed Central - PubMed

Affiliation: Stem Cell and Developmental Biology, Genome Institute of Singapore, Singapore, Singapore; Department of Biology, Clarkson University, Potsdam, New York, United States of America.

ABSTRACT
The transcription factor Zscan10 had been attributed a role as a pluripotency factor in embryonic stem cells based on its interaction with Oct4 and Sox2 in in vitro assays. Here we suggest a potential role of Zscan10 in controlling progenitor cell populations in vivo. Mice homozygous for a Zscan10 mutation exhibit reduced weight, mild hypoplasia in the spleen, heart and long bones and phenocopy an eye malformation previously described for Sox2 hypomorphs. Phenotypic abnormalities are supported by the nature of Zscan10 expression in midgestation embryos and adults suggesting a role for Zscan10 in either maintaining progenitor cell subpopulation or impacting on fate choice decisions thereof.

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Related in: MedlinePlus

Organ weight determined in pathology screen.(A) Normalized liver weight is significantly increased in male mutant mice (P = 0.008). (B) Absolute spleen weight is significantly reduced in mutant mice of both genders. (C) Normalized spleen weight is significantly reduced in female mutant mice (P = 0.002). (D) Absolute heart weight is significantly reduced in female mutant mice (p = 0.003). (E) Normalized heart weight is significantly reduced in female mutant mice (p = 0.016). All results demonstrated as boxplot with strip chart, split by sex and genotype. For tibia length refer to Figure 1F. Points are individual animals; circles represent females (f); diamonds represent males (m) of Zscan10 homozygous mutants (mut) and wild type littermates (con). Line within the boxplot represents median. Box represents the 25% and 75% quantile. Asterix represents the mean. Circled points: Values outside the upper whisker (min(max(x), 75% quantile +1.5*IQR) and outside the lower whisker (max(min(x), 25% quantile+1.5*IQR)  =  outliers. IQR  =  interquartile range (75% quantile–25% quantile).
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pone-0104568-g005: Organ weight determined in pathology screen.(A) Normalized liver weight is significantly increased in male mutant mice (P = 0.008). (B) Absolute spleen weight is significantly reduced in mutant mice of both genders. (C) Normalized spleen weight is significantly reduced in female mutant mice (P = 0.002). (D) Absolute heart weight is significantly reduced in female mutant mice (p = 0.003). (E) Normalized heart weight is significantly reduced in female mutant mice (p = 0.016). All results demonstrated as boxplot with strip chart, split by sex and genotype. For tibia length refer to Figure 1F. Points are individual animals; circles represent females (f); diamonds represent males (m) of Zscan10 homozygous mutants (mut) and wild type littermates (con). Line within the boxplot represents median. Box represents the 25% and 75% quantile. Asterix represents the mean. Circled points: Values outside the upper whisker (min(max(x), 75% quantile +1.5*IQR) and outside the lower whisker (max(min(x), 25% quantile+1.5*IQR)  =  outliers. IQR  =  interquartile range (75% quantile–25% quantile).

Mentions: In homozygous male mutant mice an increase in liver weight normalized to body weight was determined (see Figure 5A). A slightly reduced absolute and normalized spleen and heart weight in particular in female mutants was observed (see Figure 5B-E, and Figure S4), yet no morphological changes of internal organs were detected during histological analysis.


Pleiotropic functions for transcription factor zscan10.

Kraus P, V S, Yu HB, Xing X, Lim SL, Adler T, Pimentel JA, Becker L, Bohla A, Garrett L, Hans W, Hölter SM, Janas E, Moreth K, Prehn C, Puk O, Rathkolb B, Rozman J, Adamski J, Bekeredjian R, Busch DH, Graw J, Klingenspor M, Klopstock T, Neff F, Ollert M, Stoeger T, Yildrim AÖ, Eickelberg O, Wolf E, Wurst W, Fuchs H, Gailus-Durner V, de Angelis MH, Lufkin T, Stanton LW - PLoS ONE (2014)

Organ weight determined in pathology screen.(A) Normalized liver weight is significantly increased in male mutant mice (P = 0.008). (B) Absolute spleen weight is significantly reduced in mutant mice of both genders. (C) Normalized spleen weight is significantly reduced in female mutant mice (P = 0.002). (D) Absolute heart weight is significantly reduced in female mutant mice (p = 0.003). (E) Normalized heart weight is significantly reduced in female mutant mice (p = 0.016). All results demonstrated as boxplot with strip chart, split by sex and genotype. For tibia length refer to Figure 1F. Points are individual animals; circles represent females (f); diamonds represent males (m) of Zscan10 homozygous mutants (mut) and wild type littermates (con). Line within the boxplot represents median. Box represents the 25% and 75% quantile. Asterix represents the mean. Circled points: Values outside the upper whisker (min(max(x), 75% quantile +1.5*IQR) and outside the lower whisker (max(min(x), 25% quantile+1.5*IQR)  =  outliers. IQR  =  interquartile range (75% quantile–25% quantile).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4128777&req=5

pone-0104568-g005: Organ weight determined in pathology screen.(A) Normalized liver weight is significantly increased in male mutant mice (P = 0.008). (B) Absolute spleen weight is significantly reduced in mutant mice of both genders. (C) Normalized spleen weight is significantly reduced in female mutant mice (P = 0.002). (D) Absolute heart weight is significantly reduced in female mutant mice (p = 0.003). (E) Normalized heart weight is significantly reduced in female mutant mice (p = 0.016). All results demonstrated as boxplot with strip chart, split by sex and genotype. For tibia length refer to Figure 1F. Points are individual animals; circles represent females (f); diamonds represent males (m) of Zscan10 homozygous mutants (mut) and wild type littermates (con). Line within the boxplot represents median. Box represents the 25% and 75% quantile. Asterix represents the mean. Circled points: Values outside the upper whisker (min(max(x), 75% quantile +1.5*IQR) and outside the lower whisker (max(min(x), 25% quantile+1.5*IQR)  =  outliers. IQR  =  interquartile range (75% quantile–25% quantile).
Mentions: In homozygous male mutant mice an increase in liver weight normalized to body weight was determined (see Figure 5A). A slightly reduced absolute and normalized spleen and heart weight in particular in female mutants was observed (see Figure 5B-E, and Figure S4), yet no morphological changes of internal organs were detected during histological analysis.

Bottom Line: Here we suggest a potential role of Zscan10 in controlling progenitor cell populations in vivo.Mice homozygous for a Zscan10 mutation exhibit reduced weight, mild hypoplasia in the spleen, heart and long bones and phenocopy an eye malformation previously described for Sox2 hypomorphs.Phenotypic abnormalities are supported by the nature of Zscan10 expression in midgestation embryos and adults suggesting a role for Zscan10 in either maintaining progenitor cell subpopulation or impacting on fate choice decisions thereof.

View Article: PubMed Central - PubMed

Affiliation: Stem Cell and Developmental Biology, Genome Institute of Singapore, Singapore, Singapore; Department of Biology, Clarkson University, Potsdam, New York, United States of America.

ABSTRACT
The transcription factor Zscan10 had been attributed a role as a pluripotency factor in embryonic stem cells based on its interaction with Oct4 and Sox2 in in vitro assays. Here we suggest a potential role of Zscan10 in controlling progenitor cell populations in vivo. Mice homozygous for a Zscan10 mutation exhibit reduced weight, mild hypoplasia in the spleen, heart and long bones and phenocopy an eye malformation previously described for Sox2 hypomorphs. Phenotypic abnormalities are supported by the nature of Zscan10 expression in midgestation embryos and adults suggesting a role for Zscan10 in either maintaining progenitor cell subpopulation or impacting on fate choice decisions thereof.

Show MeSH
Related in: MedlinePlus