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Characterization of newly isolated lytic bacteriophages active against Acinetobacter baumannii.

Merabishvili M, Vandenheuvel D, Kropinski AM, Mast J, De Vos D, Verbeken G, Noben JP, Lavigne R, Vaneechoutte M, Pirnay JP - PLoS ONE (2014)

Bottom Line: The complete genomes of the two phages were analyzed.Genomic analysis showed that while Acibel004 represents a novel bacteriophage with resemblance to some unclassified Pseudomonas aeruginosa phages, Acibel007 belongs to the well-characterized genus of the Phikmvlikevirus.The newly isolated phages can serve as potential candidates for phage cocktails to control A. baumannii infections.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Molecular and Cellular Technology (LabMCT), Queen Astrid Military Hospital, Brussels, Belgium; Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia; Laboratory for Bacteriology Research (LBR), Faculty Medicine & Health Sciences, Ghent University, Ghent, Belgium.

ABSTRACT
Based on genotyping and host range, two newly isolated lytic bacteriophages, myovirus vB_AbaM_Acibel004 and podovirus vB_AbaP_Acibel007, active against Acinetobacter baumannii clinical strains, were selected from a new phage library for further characterization. The complete genomes of the two phages were analyzed. Both phages are characterized by broad host range and essential features of potential therapeutic phages, such as short latent period (27 and 21 min, respectively), high burst size (125 and 145, respectively), stability of activity in liquid culture and low frequency of occurrence of phage-resistant mutant bacterial cells. Genomic analysis showed that while Acibel004 represents a novel bacteriophage with resemblance to some unclassified Pseudomonas aeruginosa phages, Acibel007 belongs to the well-characterized genus of the Phikmvlikevirus. The newly isolated phages can serve as potential candidates for phage cocktails to control A. baumannii infections.

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Infection parameters of phages.A) Adsorption curve of Acibel004; B) Adsorption curve of Acibel007; C) Single-step growth curve of Acibel004; D) Single-step growth curve of Acibel007. P-number of non-adsorbed phages, P0- initial number of phages, LP-latent period, BS-burst size. The results are the mean values of three independent tests. Standard deviations are indicated.
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pone-0104853-g003: Infection parameters of phages.A) Adsorption curve of Acibel004; B) Adsorption curve of Acibel007; C) Single-step growth curve of Acibel004; D) Single-step growth curve of Acibel007. P-number of non-adsorbed phages, P0- initial number of phages, LP-latent period, BS-burst size. The results are the mean values of three independent tests. Standard deviations are indicated.

Mentions: The phage adsorption and single-step growth processes were studied to obtain an overall impression of the phage infection cycle. Acibel004 phage achieves a maximal adsorption of 85% within 15 min (Fig. 3A). A maximum of 95% of the Acibel007 phage particles absorb on the host strain within 10 min (Fig. 3B). Adsorption velocity constants for Acibel004 and Acibel007 equal to 1.2×10−9 and 1.7×10−9 ml/min, respectively.


Characterization of newly isolated lytic bacteriophages active against Acinetobacter baumannii.

Merabishvili M, Vandenheuvel D, Kropinski AM, Mast J, De Vos D, Verbeken G, Noben JP, Lavigne R, Vaneechoutte M, Pirnay JP - PLoS ONE (2014)

Infection parameters of phages.A) Adsorption curve of Acibel004; B) Adsorption curve of Acibel007; C) Single-step growth curve of Acibel004; D) Single-step growth curve of Acibel007. P-number of non-adsorbed phages, P0- initial number of phages, LP-latent period, BS-burst size. The results are the mean values of three independent tests. Standard deviations are indicated.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4128745&req=5

pone-0104853-g003: Infection parameters of phages.A) Adsorption curve of Acibel004; B) Adsorption curve of Acibel007; C) Single-step growth curve of Acibel004; D) Single-step growth curve of Acibel007. P-number of non-adsorbed phages, P0- initial number of phages, LP-latent period, BS-burst size. The results are the mean values of three independent tests. Standard deviations are indicated.
Mentions: The phage adsorption and single-step growth processes were studied to obtain an overall impression of the phage infection cycle. Acibel004 phage achieves a maximal adsorption of 85% within 15 min (Fig. 3A). A maximum of 95% of the Acibel007 phage particles absorb on the host strain within 10 min (Fig. 3B). Adsorption velocity constants for Acibel004 and Acibel007 equal to 1.2×10−9 and 1.7×10−9 ml/min, respectively.

Bottom Line: The complete genomes of the two phages were analyzed.Genomic analysis showed that while Acibel004 represents a novel bacteriophage with resemblance to some unclassified Pseudomonas aeruginosa phages, Acibel007 belongs to the well-characterized genus of the Phikmvlikevirus.The newly isolated phages can serve as potential candidates for phage cocktails to control A. baumannii infections.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Molecular and Cellular Technology (LabMCT), Queen Astrid Military Hospital, Brussels, Belgium; Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia; Laboratory for Bacteriology Research (LBR), Faculty Medicine & Health Sciences, Ghent University, Ghent, Belgium.

ABSTRACT
Based on genotyping and host range, two newly isolated lytic bacteriophages, myovirus vB_AbaM_Acibel004 and podovirus vB_AbaP_Acibel007, active against Acinetobacter baumannii clinical strains, were selected from a new phage library for further characterization. The complete genomes of the two phages were analyzed. Both phages are characterized by broad host range and essential features of potential therapeutic phages, such as short latent period (27 and 21 min, respectively), high burst size (125 and 145, respectively), stability of activity in liquid culture and low frequency of occurrence of phage-resistant mutant bacterial cells. Genomic analysis showed that while Acibel004 represents a novel bacteriophage with resemblance to some unclassified Pseudomonas aeruginosa phages, Acibel007 belongs to the well-characterized genus of the Phikmvlikevirus. The newly isolated phages can serve as potential candidates for phage cocktails to control A. baumannii infections.

Show MeSH
Related in: MedlinePlus