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Characterization of newly isolated lytic bacteriophages active against Acinetobacter baumannii.

Merabishvili M, Vandenheuvel D, Kropinski AM, Mast J, De Vos D, Verbeken G, Noben JP, Lavigne R, Vaneechoutte M, Pirnay JP - PLoS ONE (2014)

Bottom Line: The complete genomes of the two phages were analyzed.Genomic analysis showed that while Acibel004 represents a novel bacteriophage with resemblance to some unclassified Pseudomonas aeruginosa phages, Acibel007 belongs to the well-characterized genus of the Phikmvlikevirus.The newly isolated phages can serve as potential candidates for phage cocktails to control A. baumannii infections.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Molecular and Cellular Technology (LabMCT), Queen Astrid Military Hospital, Brussels, Belgium; Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia; Laboratory for Bacteriology Research (LBR), Faculty Medicine & Health Sciences, Ghent University, Ghent, Belgium.

ABSTRACT
Based on genotyping and host range, two newly isolated lytic bacteriophages, myovirus vB_AbaM_Acibel004 and podovirus vB_AbaP_Acibel007, active against Acinetobacter baumannii clinical strains, were selected from a new phage library for further characterization. The complete genomes of the two phages were analyzed. Both phages are characterized by broad host range and essential features of potential therapeutic phages, such as short latent period (27 and 21 min, respectively), high burst size (125 and 145, respectively), stability of activity in liquid culture and low frequency of occurrence of phage-resistant mutant bacterial cells. Genomic analysis showed that while Acibel004 represents a novel bacteriophage with resemblance to some unclassified Pseudomonas aeruginosa phages, Acibel007 belongs to the well-characterized genus of the Phikmvlikevirus. The newly isolated phages can serve as potential candidates for phage cocktails to control A. baumannii infections.

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Related in: MedlinePlus

Transmission electron micrographs of phages.A) Acibel004, member of the Myoviridae family; B) Acibel007, member of the Podoviridae family.
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pone-0104853-g002: Transmission electron micrographs of phages.A) Acibel004, member of the Myoviridae family; B) Acibel007, member of the Podoviridae family.

Mentions: Acibel004 and Acibel007 phages form clear plaques on the host strain, differing in size. Acibel004 phage forms plaques with a diameter of 1–2 mm, compared to 3–5 mm for Acibel007. Phage particle morphology examined by TEM revealed that Acibel004 and Acibel007 can be assigned to different families of the order Caudovirales (Fig. 2). Acibel004, with a 70 nm icosahedral head and a 105 nm long contractile tail, is classified as morphotype A1 of the Myoviridae, while Acibel007, with a 60 nm head and a 10 nm long noncontractile tail, belongs to morphotype C1 of the Podoviridae.


Characterization of newly isolated lytic bacteriophages active against Acinetobacter baumannii.

Merabishvili M, Vandenheuvel D, Kropinski AM, Mast J, De Vos D, Verbeken G, Noben JP, Lavigne R, Vaneechoutte M, Pirnay JP - PLoS ONE (2014)

Transmission electron micrographs of phages.A) Acibel004, member of the Myoviridae family; B) Acibel007, member of the Podoviridae family.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4128745&req=5

pone-0104853-g002: Transmission electron micrographs of phages.A) Acibel004, member of the Myoviridae family; B) Acibel007, member of the Podoviridae family.
Mentions: Acibel004 and Acibel007 phages form clear plaques on the host strain, differing in size. Acibel004 phage forms plaques with a diameter of 1–2 mm, compared to 3–5 mm for Acibel007. Phage particle morphology examined by TEM revealed that Acibel004 and Acibel007 can be assigned to different families of the order Caudovirales (Fig. 2). Acibel004, with a 70 nm icosahedral head and a 105 nm long contractile tail, is classified as morphotype A1 of the Myoviridae, while Acibel007, with a 60 nm head and a 10 nm long noncontractile tail, belongs to morphotype C1 of the Podoviridae.

Bottom Line: The complete genomes of the two phages were analyzed.Genomic analysis showed that while Acibel004 represents a novel bacteriophage with resemblance to some unclassified Pseudomonas aeruginosa phages, Acibel007 belongs to the well-characterized genus of the Phikmvlikevirus.The newly isolated phages can serve as potential candidates for phage cocktails to control A. baumannii infections.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Molecular and Cellular Technology (LabMCT), Queen Astrid Military Hospital, Brussels, Belgium; Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia; Laboratory for Bacteriology Research (LBR), Faculty Medicine & Health Sciences, Ghent University, Ghent, Belgium.

ABSTRACT
Based on genotyping and host range, two newly isolated lytic bacteriophages, myovirus vB_AbaM_Acibel004 and podovirus vB_AbaP_Acibel007, active against Acinetobacter baumannii clinical strains, were selected from a new phage library for further characterization. The complete genomes of the two phages were analyzed. Both phages are characterized by broad host range and essential features of potential therapeutic phages, such as short latent period (27 and 21 min, respectively), high burst size (125 and 145, respectively), stability of activity in liquid culture and low frequency of occurrence of phage-resistant mutant bacterial cells. Genomic analysis showed that while Acibel004 represents a novel bacteriophage with resemblance to some unclassified Pseudomonas aeruginosa phages, Acibel007 belongs to the well-characterized genus of the Phikmvlikevirus. The newly isolated phages can serve as potential candidates for phage cocktails to control A. baumannii infections.

Show MeSH
Related in: MedlinePlus