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Altered distribution of peripheral blood memory B cells in humans chronically infected with Trypanosoma cruzi.

Fernández ER, Olivera GC, Quebrada Palacio LP, González MN, Hernandez-Vasquez Y, Sirena NM, Morán ML, Ledesma Patiño OS, Postan M - PLoS ONE (2014)

Bottom Line: Infected individuals had decreased frequencies of CD19+CD27+ cells, which positively correlated with the frequencies of CD4+CD27+ cells.Elevated levels of serum soluble CD27 were observed in infected individuals with Chagas cardiomyopathy, indicating its potentiality as an immunological marker for disease progression in endemic areas.In conclusion, our results demonstrate that chronic T. cruzi infection alters the distribution of various peripheral blood B cell subsets, probably related to the CD4+ T cell deregulation process provoked by the parasite in humans.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Investigación, Instituto Nacional de Parasitología "Dr. Mario Fatala Chabén", Ciudad Autónoma de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.

ABSTRACT
Numerous abnormalities of the peripheral blood T cell compartment have been reported in human chronic Trypanosoma cruzi infection and related to prolonged antigenic stimulation by persisting parasites. Herein, we measured circulating lymphocytes of various phenotypes based on the differential expression of CD19, CD4, CD27, CD10, IgD, IgM, IgG and CD138 in a total of 48 T. cruzi-infected individuals and 24 healthy controls. Infected individuals had decreased frequencies of CD19+CD27+ cells, which positively correlated with the frequencies of CD4+CD27+ cells. The contraction of CD19+CD27+ cells was comprised of IgG+IgD-, IgM+IgD- and isotype switched IgM-IgD- memory B cells, CD19+CD10+CD27+ B cell precursors and terminally differentiated CD19+CD27+CD138+ plasma cells. Conversely, infected individuals had increased proportions of CD19+IgG+CD27-IgD- memory and CD19+IgM+CD27-IgD+ transitional/naïve B cells. These observations prompted us to assess soluble CD27, a molecule generated by the cleavage of membrane-bound CD27 and used to monitor systemic immune activation. Elevated levels of serum soluble CD27 were observed in infected individuals with Chagas cardiomyopathy, indicating its potentiality as an immunological marker for disease progression in endemic areas. In conclusion, our results demonstrate that chronic T. cruzi infection alters the distribution of various peripheral blood B cell subsets, probably related to the CD4+ T cell deregulation process provoked by the parasite in humans.

No MeSH data available.


Related in: MedlinePlus

Comparison of serum sCD27 levels in individuals chronically infected with Trypanosoma cruzi.The levels of sCD27 were measured by ELISA in individuals with different degrees of cardiac dysfunction and controls. Bars represent the mean and SD for each group. Statistical significance was determined with One-way ANOVA followed by Bonferroni’s post-test. *p<0.05. ƒ N, normal and A, abnormal.
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pone-0104951-g004: Comparison of serum sCD27 levels in individuals chronically infected with Trypanosoma cruzi.The levels of sCD27 were measured by ELISA in individuals with different degrees of cardiac dysfunction and controls. Bars represent the mean and SD for each group. Statistical significance was determined with One-way ANOVA followed by Bonferroni’s post-test. *p<0.05. ƒ N, normal and A, abnormal.

Mentions: sCD27 has been related to membrane CD27 expression on activated lymphocytes, and used to monitor systemic immune activation in a variety of immunological disorders such as chronic viral infections, autoimmune diseases and B cell malignancies [22], [23], [42], [43]. Here we measured the levels of serum sCD27 in T. cruzi-infected individuals with different degrees of cardiac dysfunction. Individuals with Chagas cardiomyopathy had increased levels of sCD27 as compared to the other groups of infected subjects and controls (P = 0.0034; Fig. 4). No correlation between the levels of sCD27 and CD19+CD27+ or CD4+CD27+ cells was found. The observation of elevated levels of sCD27 in association with Chagas cardiomyopathy is of great interest for monitoring disease progression in endemic areas of Chagas disease, as the ELISA technique used to determine the levels of sCD27 is much simpler to perform than flow cytometry.


Altered distribution of peripheral blood memory B cells in humans chronically infected with Trypanosoma cruzi.

Fernández ER, Olivera GC, Quebrada Palacio LP, González MN, Hernandez-Vasquez Y, Sirena NM, Morán ML, Ledesma Patiño OS, Postan M - PLoS ONE (2014)

Comparison of serum sCD27 levels in individuals chronically infected with Trypanosoma cruzi.The levels of sCD27 were measured by ELISA in individuals with different degrees of cardiac dysfunction and controls. Bars represent the mean and SD for each group. Statistical significance was determined with One-way ANOVA followed by Bonferroni’s post-test. *p<0.05. ƒ N, normal and A, abnormal.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4128741&req=5

pone-0104951-g004: Comparison of serum sCD27 levels in individuals chronically infected with Trypanosoma cruzi.The levels of sCD27 were measured by ELISA in individuals with different degrees of cardiac dysfunction and controls. Bars represent the mean and SD for each group. Statistical significance was determined with One-way ANOVA followed by Bonferroni’s post-test. *p<0.05. ƒ N, normal and A, abnormal.
Mentions: sCD27 has been related to membrane CD27 expression on activated lymphocytes, and used to monitor systemic immune activation in a variety of immunological disorders such as chronic viral infections, autoimmune diseases and B cell malignancies [22], [23], [42], [43]. Here we measured the levels of serum sCD27 in T. cruzi-infected individuals with different degrees of cardiac dysfunction. Individuals with Chagas cardiomyopathy had increased levels of sCD27 as compared to the other groups of infected subjects and controls (P = 0.0034; Fig. 4). No correlation between the levels of sCD27 and CD19+CD27+ or CD4+CD27+ cells was found. The observation of elevated levels of sCD27 in association with Chagas cardiomyopathy is of great interest for monitoring disease progression in endemic areas of Chagas disease, as the ELISA technique used to determine the levels of sCD27 is much simpler to perform than flow cytometry.

Bottom Line: Infected individuals had decreased frequencies of CD19+CD27+ cells, which positively correlated with the frequencies of CD4+CD27+ cells.Elevated levels of serum soluble CD27 were observed in infected individuals with Chagas cardiomyopathy, indicating its potentiality as an immunological marker for disease progression in endemic areas.In conclusion, our results demonstrate that chronic T. cruzi infection alters the distribution of various peripheral blood B cell subsets, probably related to the CD4+ T cell deregulation process provoked by the parasite in humans.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Investigación, Instituto Nacional de Parasitología "Dr. Mario Fatala Chabén", Ciudad Autónoma de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.

ABSTRACT
Numerous abnormalities of the peripheral blood T cell compartment have been reported in human chronic Trypanosoma cruzi infection and related to prolonged antigenic stimulation by persisting parasites. Herein, we measured circulating lymphocytes of various phenotypes based on the differential expression of CD19, CD4, CD27, CD10, IgD, IgM, IgG and CD138 in a total of 48 T. cruzi-infected individuals and 24 healthy controls. Infected individuals had decreased frequencies of CD19+CD27+ cells, which positively correlated with the frequencies of CD4+CD27+ cells. The contraction of CD19+CD27+ cells was comprised of IgG+IgD-, IgM+IgD- and isotype switched IgM-IgD- memory B cells, CD19+CD10+CD27+ B cell precursors and terminally differentiated CD19+CD27+CD138+ plasma cells. Conversely, infected individuals had increased proportions of CD19+IgG+CD27-IgD- memory and CD19+IgM+CD27-IgD+ transitional/naïve B cells. These observations prompted us to assess soluble CD27, a molecule generated by the cleavage of membrane-bound CD27 and used to monitor systemic immune activation. Elevated levels of serum soluble CD27 were observed in infected individuals with Chagas cardiomyopathy, indicating its potentiality as an immunological marker for disease progression in endemic areas. In conclusion, our results demonstrate that chronic T. cruzi infection alters the distribution of various peripheral blood B cell subsets, probably related to the CD4+ T cell deregulation process provoked by the parasite in humans.

No MeSH data available.


Related in: MedlinePlus