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Prevalence of HPV 16 and HPV 18 lineages in Galicia, Spain.

Pérez S, Cid A, Iñarrea A, Pato M, Lamas MJ, Couso B, Gil M, Alvarez MJ, Rey S, López-Miragaya I, Melón S, Oña Md - PLoS ONE (2014)

Bottom Line: In conclusion, lineages A of HPV16/18 were predominant in Spain.Lineage D of HPV16 was associated with increased risk for CIN3+, glandular high-grade lesions, and invasive lesions compared with lineage A.Lineage B of HPV18 may be associated with increased risk for CIN3+ compared with lineage A, but the association was not significant.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University Hospital of Vigo, Vigo, Spain.

ABSTRACT
Genetic variants of human papillomavirus types 16 and 18 (HPV16/18) could differ in their cancer risk. We studied the prevalence and association with high-grade cervical lesions of different HPV16/18 variant lineages in a case-control study including 217 cases (cervical intraepithelial neoplasia grade 2 or grade 3 or worse: CIN2 or CIN3+) and 116 controls (no CIN2 or CIN3+ in two-year follow-up). HPV lineages were determined by sequencing the long control region (LCR) and the E6 gene. Phylogenetic analysis of HPV16 confirmed that isolates clustered into previously described lineages: A (260, 87.5%), B (4, 1.3%), C (8, 2.7%), and D (25, 8.4%). Lineage D/lineage A strains were, respectively, detected in 4/82 control patients, 19/126 CIN3+ cases (OR = 3.1, 95%CI: 1.0-12.9, p = 0.04), 6/1 glandular high-grade lesions (OR = 123, 95%CI: 9.7-5713.6, p<0.0001), and 4/5 invasive lesions (OR = 16.4, 95%CI: 2.2-113.7, p = 0.002). HPV18 clustered in lineages A (32, 88.9%) and B (4, 11.1%). Lineage B/lineage A strains were respectively detected in 1/23 control patients and 2/5 CIN3+ cases (OR = 9.2, 95%CI: 0.4-565.4, p = 0.12). In conclusion, lineages A of HPV16/18 were predominant in Spain. Lineage D of HPV16 was associated with increased risk for CIN3+, glandular high-grade lesions, and invasive lesions compared with lineage A. Lineage B of HPV18 may be associated with increased risk for CIN3+ compared with lineage A, but the association was not significant. Large well-designed studies are needed before the application of HPV lineage detection in clinical settings.

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Nucleotide sequence variations of L1 among HPV16 isolates.Position number refers to the HPV 16 prototype sequence previously described. Asterisk indicates amino acid substitution.
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pone-0104678-g006: Nucleotide sequence variations of L1 among HPV16 isolates.Position number refers to the HPV 16 prototype sequence previously described. Asterisk indicates amino acid substitution.

Mentions: Sequencing data of the capsid region were obtained for a total of 250 women infected by HPV16 included in the study, and with a valid follow-up. Nucleotide sequence variations of L1 among HPV16 isolates are shown in figure 6. Genetic variability analysis of L1 region revealed 21 silent nucleotide substitutions and eight nucleotide variations that lead to amino acid changes. The two more frequent non-synonymous SNPs were 6695C (27/250, 10.8%) and 6803T (17/250, 6.8%). The SNP 6803T was exclusively detected in strains previously characterized as lineage D by the LCR/E6 region analysis. The 6803A and 6803T strains were respectively found in 3/88 control patients, 5/1 glandular high-grade lesions (OR = 146.7, 95%CI: 9.9–6996.7, p<0.0001), and 4/4 invasive lesions (OR = 29.3, 95%CI: 3.4–254.6, p<0.001).


Prevalence of HPV 16 and HPV 18 lineages in Galicia, Spain.

Pérez S, Cid A, Iñarrea A, Pato M, Lamas MJ, Couso B, Gil M, Alvarez MJ, Rey S, López-Miragaya I, Melón S, Oña Md - PLoS ONE (2014)

Nucleotide sequence variations of L1 among HPV16 isolates.Position number refers to the HPV 16 prototype sequence previously described. Asterisk indicates amino acid substitution.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4128731&req=5

pone-0104678-g006: Nucleotide sequence variations of L1 among HPV16 isolates.Position number refers to the HPV 16 prototype sequence previously described. Asterisk indicates amino acid substitution.
Mentions: Sequencing data of the capsid region were obtained for a total of 250 women infected by HPV16 included in the study, and with a valid follow-up. Nucleotide sequence variations of L1 among HPV16 isolates are shown in figure 6. Genetic variability analysis of L1 region revealed 21 silent nucleotide substitutions and eight nucleotide variations that lead to amino acid changes. The two more frequent non-synonymous SNPs were 6695C (27/250, 10.8%) and 6803T (17/250, 6.8%). The SNP 6803T was exclusively detected in strains previously characterized as lineage D by the LCR/E6 region analysis. The 6803A and 6803T strains were respectively found in 3/88 control patients, 5/1 glandular high-grade lesions (OR = 146.7, 95%CI: 9.9–6996.7, p<0.0001), and 4/4 invasive lesions (OR = 29.3, 95%CI: 3.4–254.6, p<0.001).

Bottom Line: In conclusion, lineages A of HPV16/18 were predominant in Spain.Lineage D of HPV16 was associated with increased risk for CIN3+, glandular high-grade lesions, and invasive lesions compared with lineage A.Lineage B of HPV18 may be associated with increased risk for CIN3+ compared with lineage A, but the association was not significant.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University Hospital of Vigo, Vigo, Spain.

ABSTRACT
Genetic variants of human papillomavirus types 16 and 18 (HPV16/18) could differ in their cancer risk. We studied the prevalence and association with high-grade cervical lesions of different HPV16/18 variant lineages in a case-control study including 217 cases (cervical intraepithelial neoplasia grade 2 or grade 3 or worse: CIN2 or CIN3+) and 116 controls (no CIN2 or CIN3+ in two-year follow-up). HPV lineages were determined by sequencing the long control region (LCR) and the E6 gene. Phylogenetic analysis of HPV16 confirmed that isolates clustered into previously described lineages: A (260, 87.5%), B (4, 1.3%), C (8, 2.7%), and D (25, 8.4%). Lineage D/lineage A strains were, respectively, detected in 4/82 control patients, 19/126 CIN3+ cases (OR = 3.1, 95%CI: 1.0-12.9, p = 0.04), 6/1 glandular high-grade lesions (OR = 123, 95%CI: 9.7-5713.6, p<0.0001), and 4/5 invasive lesions (OR = 16.4, 95%CI: 2.2-113.7, p = 0.002). HPV18 clustered in lineages A (32, 88.9%) and B (4, 11.1%). Lineage B/lineage A strains were respectively detected in 1/23 control patients and 2/5 CIN3+ cases (OR = 9.2, 95%CI: 0.4-565.4, p = 0.12). In conclusion, lineages A of HPV16/18 were predominant in Spain. Lineage D of HPV16 was associated with increased risk for CIN3+, glandular high-grade lesions, and invasive lesions compared with lineage A. Lineage B of HPV18 may be associated with increased risk for CIN3+ compared with lineage A, but the association was not significant. Large well-designed studies are needed before the application of HPV lineage detection in clinical settings.

Show MeSH
Related in: MedlinePlus